Genetic diversity and antigenicity variation of  Babesia bovis  merozoite surface antigen-1 (MSA-1) in Thailand

Tattiyapong, Muncharee; Sivakumar, Thillaiampalam; Takemae, Hitoshi; Simking, P.; Jittapalapong, Sathaporn; Igarashi, Ikuo; Yokoyama, Naoki

doi:10.1016/j.meegid.2016.04.021

Cited by 13 publications

(12 citation statements)

References 35 publications

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“…Cattle, as well as buffaloes, have been screened with highly sensitive PCR assays to detect persistently infected animals in order to genetically characterize the Merozoite Surface Antigen (MSA-1) diversity in Babesia bovis populations present in cattle premises from Mexico [20], Thailand, Brazil and Ghana [21], Vietnam [22], Thailand [23], Sri Lanka, Mongolia and Vietnam [24].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Tools for the Identification of Babesia sp. in Persistently Infected Cattle

2019

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Bovine babesiosis is a tick-borne disease of cattle caused by the protozoan parasites of the genus Babesia. Babesia bovis, Babesia bigemina and Babesia divergens are considered by International health authorities (OIE) as the principal species of Babesia that cause bovine babesiosis. Animals that recover from a babesial primo infection may remain as persistent carriers with no clinical signs of disease and can be the source of infection for ticks that are able to acquire Babesia parasites from infected cattle and to transmit Babesia parasites to susceptible cattle. Several procedures that have been developed for parasite detection and diagnosis of this infectious carrier state constitute the basis for this review: A brief description of the direct microscopic detection of Babesia-infected erytrocytes; PCR-based diagnostic assays, which are very sensitive particularly in detecting Babesia in carrier cattle; in-vitro culture methods, used to demonstrate presence of carrier infections of Babesia sp.; animal inoculation, particularly for B. divergens isolation are discussed. Alternatively, persistently infected animals can be tested for specific antibabesial antibodies by using indirect serological assays. Serological procedures are not necessarily consistent in identifying persistently infected animals and have the disadvantage of presenting with cross reactions between antibodies to Babesia sp.

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Section: Introductionmentioning

confidence: 99%

Diagnostic Tools for the Identification of Babesia sp. in Persistently Infected Cattle

2019

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“…Antigenic polymorphism has been applied as a strategy by most protozoan parasites to escape the immune response of mammalian hosts, and it is a major obstacle preventing the development of effective vaccines against these parasites [7]. In our present study, analysis of the genetic relationships of B. bovis MSA-1, MSA-2b and MSA-2c of Chinese isolates, with respect to almost all available related sequences deposited in the GenBank database (including Mongolia, Vietnam, Philippines, Thailand, Israel, Vietnam, Sri Lanka, Mexico, Argentina, Brazil, Japan and USA) was conducted [7,12,[18][19][20][21][22][23][24]26,30,31]. Phylogenetic analysis showed that the MSA-1 amino acid sequences had a wide distribution in four clades.…”

Section: Discussionmentioning

confidence: 99%

“…Phylogenetic analyses of B. bovis MSAs have been performed in several countries, including Mongolia, Vietnam, the Philippines, Thailand, Israel, Vietnam, Sri Lanka, Mexico, Argentina and Brazil [7,12,[18][19][20][21][22][23][24][25][26]. In China, molecular epidemiological investigations of B. bovis infection have been performed in several provinces [4,5].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Babesia bovis MSA-1, MSA-2b and MSA-2c in China

et al. 2020

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The apicomplexan parasite Babesia bovis is a tick-borne intracellular hemoprotozoan parasite that is widespread across China. Genetic diversity is an important strategy used by parasites to escape the immune responses of their hosts. In our present study, 575 blood samples, collected from cattle in 10 provinces, were initially screened using a nested PCR (polymerase chain reaction) for detection of B. bovis infection. To perform genetic diversity analyses, positive samples were further amplified to obtain sequences of three B. bovis merozoite surface antigen genes (MSA-1, MSA-2b, MSA-2c). The results of the nested PCR approach showed that an average of 8.9% (51/575) of cattle were positive for B. bovis infection. Phylogenetic analyses of the predicted amino acid sequences revealed that unique antigen variants were formed only by Chinese isolates. Our findings provide vital information for understanding the genetic diversity of B. bovis in China.

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“…Among those families mentioned above, the rap1 , msa1 , sbp2 , and 6-cys multigene families and their encoded proteins fall into the variable category. The diversity present in variable genes coupled with their relative stability make them good markers for use in epidemiologic studies at the isolate level, and advantage has been taken of rap1 and msa1 sequences repeatedly for this purpose (for example, see [21,22,23,24,25,26,27,28,29,30]). In contrast, members of the ves multigene family are highly variant in sequence and the expressed member(s) further vary rapidly during the course of infection in an individual host [14].…”

Section: Major Variable and Variant Multigene Protein Familiesmentioning

confidence: 99%

Variable and Variant Protein Multigene Families in Babesia bovis Persistence

Allred

2019

Pathogens

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Cattle infected with Babesia bovis face a bifurcated fate: Either die of the severe acute infection, or survive and carry for many years a highly persistent but generally asymptomatic infection. In this review, the author describes known and potential contributions of three variable or highly variant multigene-encoded families of proteins to persistence in the bovine host, and the mechanisms by which variability arises among these families. Ramifications arising from this variability are discussed.

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Genetic diversity and antigenicity variation of Babesia bovis merozoite surface antigen-1 (MSA-1) in Thailand

Cited by 13 publications

References 35 publications

Diagnostic Tools for the Identification of Babesia sp. in Persistently Infected Cattle

Diagnostic Tools for the Identification of Babesia sp. in Persistently Infected Cattle

Genetic Diversity of Babesia bovis MSA-1, MSA-2b and MSA-2c in China

Variable and Variant Protein Multigene Families in Babesia bovis Persistence

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