Genetic diversity and antifungal susceptibility of Candida albicans isolated from Iranian patients

Abstract

A total of 105 independent Candida albicans strains isolated from patients in Iran were investigated. According to CLSI documents M27-A3 and M27-S4, the 24 h geometric mean MICs of caspofungin, itraconazole, and fluconazole were 0.27, 3.19, and 11.91 μg/ml, respectively. Microsatellites analysis of CEF3, CAIII, LOC4 Loci identified 93 different allelic genotypes clustered apart into six different clades. Antifungal susceptibility was not linked with the source of isolation and the corresponding genotype of C. albicans strains.

Genotyping and Drug Resistance Profile of Clinical Isolates of Candida albicans from Vulvovaginal Candidiasis in the Eastern China

“…Moreover, a total of 108 isolates were shown to represent unique molecular types which account for 44% of the isolates tested, indicating high genetic diversity within the isolates in this study (Supplementary table 1). Our results confirmed the high genetic diversity among C. albicans isolates reported in previous similar studies [ 10 , 11 ]. Sharifynia et al .…”

“…Moreover, a total of 108 isolates were shown to represent unique molecular types which account for 44% of the isolates tested, indicating high genetic diversity within the isolates in this study (Supplementary table 1). Our results confirmed the high genetic diversity among C. albicans isolates reported in previous similar studies [10,11]. Sharifynia et al [11] analyzed the different allelic combinations of 3 microsatellite loci (CAIII, CEF3, and LOC) in 105 independent C. albicans strains isolated from patients in Iran, and identified 93 unique microsatellite genotypes that clustered into six clades.…”

“…Our results confirmed the high genetic diversity among C. albicans isolates reported in previous similar studies [10,11]. Sharifynia et al [11] analyzed the different allelic combinations of 3 microsatellite loci (CAIII, CEF3, and LOC) in 105 independent C. albicans strains isolated from patients in Iran, and identified 93 unique microsatellite genotypes that clustered into six clades. Garcia-Hermoso et al [28] identified 38 different genotypes among 50 C. albicans strains in a surgical intensive care unit using the microsatellite analysis of EF3, CDC3 and HIS3 loci.…”

“…Microsatellite genotyping was performed with all 244 C. albicans isolates, based on a panel of three different short-nucleotide repeat fragments, using fluorescently labeled primers CAIII (5 0 -Tamra -TTGGAAT-CACTTCACCAGGA-3 0 , 5 0 -TTTCCGTGGCAT-CAGTATCA-3 0 ); CEF3 (5 0 -Hex-TTTCCTCTTCCT TTCATATAGAA-3 0 , 5 0 -GGATTCACTAGCAGCA-GACA-3 0 ); LOC4 (5 0 -FAM -GTAATGATTACGG-CAATGAC-3 0 , 5 0 -AGAACGACGTGTACTATTGG-3 0 ) [11]. A multiplex polymerase chain reaction (PCR) was performed in 10 ll reaction volumes containing 5 ll of Qiagen Multiplex PCR (2x, Lot 148031955), 0.25 ll of each primer (forward and reverse), 3 ll of ddH 2 O, and 1 ll of genomic DNA.…”

“…Microsatellite analysis is highly reproducible with strong discriminatory power and is widely applied in molecular typing of fungal pathogens [ 9 ]. In previous studies, microsatellite analysis has proved to be a powerful tool in investigating the relationship between genetic diversity and antifungal susceptibility of C. albicans isolates [ 10 , 11 ]. However, knowledge on the population genetic structure and antifungal susceptibility profile of isolates from VVC patients in China still remains limited.…”