Genetic Diversity Analysis of the Gohilwari Breed of Indian Goat (Capra hircus) Using Microsatellite Markers

Kumar, Satish; Dixit, S. P.; Verma, Naresh K.; Singh, Dharmendra; Pande, Anjali; Chander, Ramesh; Singh, Lalji

doi:10.3844/ajavsp.2009.49.57

Cited by 38 publications

(23 citation statements)

References 35 publications

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“…The presence of long term natural selection for adaptation and the existence of interbreeding as result of the free movement of animals in the area are thought to contribute at large to the broad genetic diversity of the Syrian goats. The genetic diversity observed in Syrian goat breeds was comparable with the results reported on South-East Asian goats (Barker et al, 2001), Chegu goats (Behl et al, 2003), Indian goats (Kumar et al, 2009) and Ethiopian goats (Hassen et al, 2012). In addition, the PIC values across all loci ranged from 0.71 (for Jabali goats) to 0.83 (for Shami goats), with an average PIC value of 0.78 (Table 4), which suggested that all loci used in this study showed relatively high polymorphism for analyzing genetic diversity in goats.…”

Section: Genetic Diversity and Differentiationsupporting

confidence: 86%

Morphological and molecular genetic diversity of Syrian indigenous goat populations

Hassen¹,

Rischkowsky²,

Termanini³

et al. 2016

Afr. J. Biotechnol.

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Domestic goats in Syria may provide an interesting source of genetic variability due to its proximity to the centers of domestication. This study aimed to assess the morphological variation, genetic diversity and population substructure of the Syrian goat populations. Commonly, three goat genotypes are distinguished in Syria, namely Jabali or mountain goat, Baladi or local goat and Shami or Damascus (a well-known dairy goat). A pre-tested semi-structured questionnaire was used in recording both qualitative (coat color, eye color, horn length, horn orientation, nose profile) and quantitative (height at wither, chest girth, cannon length, body length, ear length and ear width) morphological data. Data from a total of 5,730 individual goats of the three goat populations reared in ten representative provinces of Syria were collected and analyzed using GenStat version 14 statistical packages. Results of the morphological analysis confirmed that there were clear morphological variations among the three goat populations. The three goat populations are mainly distinguished by their straight (Baladi, 71.1% and Jabali, 82.8%) and curved (Shami, 89.5%) nose profiles. Substantial phenotypic variability was found among and within the breeds suggesting that these goat breeds have not yet undergone an organized breeding program. The genetic variability and population substructures from 398 individual animals of the three breeds were genotyped using 12 DNA microsatellite markers from Food and Agricultural Organization (FAO) panel. All microsatellites typed were found to be polymorphic and a total of 41 distinct alleles were detected on Baladi, Jabali and Shami goat populations. The Syrian goat populations had observed and expected heterozygosity values that ranged from 0.50 to 0.62 and 0.74 to 0.85, respectively, and an average of 13.97 alleles per locus across breeds. For all loci, an average inbreeding values (F IS ) of low to moderate level was obtained across the three goat breeds, which ranged from 0.29 (Shami goats) to 0.34 (Baladi goats) indicating the absence of mating between close relatives within these populations. The observed positive F IS coefficients among the studied goat breeds also suggested heterozygote deficiencies. The analyses of the molecular data using STRUCURE program indicated there were two primary populations, which did support the results based on morphological data of the same goat populations that clustered these goat populations into two main groups and confirmed the admixture nature of the Baladi and Jabali goat populations, while the Shami goat breed was well differentiated and grouped into a separate cluster that suggests its evolutionary and genetic uniqueness. The analysis of molecular variance (AMOVA) results detected genetic variations within individuals in a population (96%). The high genetic variability within individuals in a population provides a good base for designing genetic improvement programs under the existing goat management systems.

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Section: Genetic Diversity and Differentiationsupporting

confidence: 86%

Morphological and molecular genetic diversity of Syrian indigenous goat populations

Hassen¹,

Rischkowsky²,

Termanini³

et al. 2016

Afr. J. Biotechnol.

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“…The different measures of genetic variation such as Na, Ne and PIC showed that all of the studied loci were highly informative, indicating high polymorphism across the loci, thus suggesting suitability of these markers for the study of genetic diversity in goats. The overall mean of gene diversity (0.73) was higher than the value reported in Gohilwadi (0.68) goats for the same panel of microsatellite markers (Kumar et al, 2009) and in Swiss goat breeds (0.58) for 20 microsatellite loci (Saitbekova et al, 1999). The F-statistics were applied to know the extent of inbreeding and outbreeding in the population (Table 1).…”

Section: Genetic Variabilitymentioning

confidence: 96%

“…Suitability of these markers was further supported by the fact that the number of alleles for each marker was higher than the minimum number of alleles (four) recommended for microsatellite markers to be used in the estimation of genetic distance in order to reduce the standard error. The Konkan Kanyal goat population exhibited higher mean observed heterozygosity than Gohilwadi (Kumar et al, 2009), Kutchi (Dixit et al, 2008) and many other Asian goats but lower than that of Kanniadu breed of goat (Thilagam et al, 2006). The Fis value provides the non-random union of gametes in the population, i.e.…”

Section: Genetic Variabilitymentioning

confidence: 99%

Genetic diversity and bottleneck analysis of Konkan Kanyal goats

Mishra

Ali²,

Aggarwal

et al. 2012

Anim. Genet. Resour.

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Konkan Kanyal goats are medium-sized animals reared for meat purpose. They are predominantly black in colour with white markings in a specific pattern and are found in the Kudal, Sawantwadi, Dodamarg, Malvan and Vengurle Talukas of Sindhudurg district of Konkan region of Maharashtra state. The mean body weight ranges from 30 kg in adult females to 35 kg in males. The age at first kidding, kidding interval and twinning of these goats is 546 days, 240 days and 35 percent respectively. The average daily milk yield and lactation yield were found to be 0.6 and 69 kg, respectively. Characterization of Konkan Kanyal goats was carried out using a panel of 25 microsatellite markers. The genomic DNA of 50 unrelated goats was isolated and PCR amplified under standardized PCR conditions. The amplified DNA was used for genotyping by the automated DNA Sequencer ABI 3730. The analysis of data revealed that the effective number of alleles ranged from 1.17 to 8.94 and the number of observed alleles ranged from 5 to 19. A total of 255 alleles were observed with a mean of 10.2 alleles/locus. The observed heterozygosity ranged from 0.13 to 0.95, while the expected heterozygosity ranged from 0.14 to 0.89, indicating the heterogeneous nature of the population distributed in the breeding tract. The mean polymorphism content was 0.79. The qualitative graphical method based on the allele frequency spectra detected no shift in the frequency distribution of alleles and a normal L-shaped curve was observed where the alleles with the lowest frequencies were found to be most abundant. Based on the phenotypic and genetic variability, Konkan Kanyal goats appear to be distinct from other goats of Maharashtra state. RésuméLes chèvres Konkan Kanyal, de taille moyenne, sont élevées pour leur viande. Elles sont principalement de couleur noire avec des taches blanches ayant un motif caractéristique et vivent dans les unités administratives de Kudal, Sawantwadi, Dodamarg, Malvan et Vengurle du district de Sindhudurg sur la côte de Konkan dans l'État du Maharashtra. La moyenne de leur poids corporel varie entre 29,80 kg pour les femelles adultes et 35,26 kg pour les mâles. L'âge au premier agnelage et l'intervalle entre les agnelages sont respectivement de 546 jours et de 240 jours. Trente-cinq pour cent des naissances de ces chèvres sont gémellaires. On a constaté que le rendement moyen de lait par jour et la quantité de lait donnée au cours d'une lactation sont respectivement de 0,6 kg et de 69 kg. La caractérisation des chèvres Konkan Kanyal a été réalisée en utilisant un panel de vingt-cinq marqueurs microsatellites. L'ADN génomique de 50 chèvres sans liens de parenté a été isolé et amplifié selon des méthodes PCR standardisées. L'ADN amplifié a été utilisé pour le génotypage par le biais du séquenceur automatisé d'ADN ABI 3730. L'analyse des données a indiqué que le nombre réel d'allèles varie entre 1,17 et 8,94 et le nombre d'allèles observés entre 5 et 19. On a constaté au total 255allèles avec une moyenne de 10,2 allèles par locus. L'hétérozygo...

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“…Genetic diversity is an essential component for population survival, evolution, genetic improvement and adaptation to changing environmental conditions (Kumar et al, 2009). The conservation of genetic variability has arisen in the last years because the existence of a large gene pool is important for the potential future breeding preservation and for sustainable animal production system development.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and distance of Iranian goat breeds (Markhoz, Mahabadi and Lori) compared to the Beetal breed using inter-simple sequence repeat (ISSR) markers

et al. 2016

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Abstract. The aim of this study was to investigate the genetic diversity and structure in three Iranian native goat breeds (Markhoz, Mahabadi and Lori) and the Beetal imported breed using inter-simple sequence repeat (ISSR) markers and also to investigate ISSR markers' potential in order to genetically separate single (S) and twin-birth (T) subpopulations. Blood samples were collected from 210 animals for this purpose. In total, 16 primers were used, and finally 5 primers were selected based on the number of clear bands and the level of polymorphisms. The result of this study showed that 76 of 86 observed fragments were polymorphic. Genetic diversity for each breed ranged from 0.23 in the Beetal breed to 0.26 in the Markhoz breed; this represents a relatively similar genetic diversity in these breeds. An unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on the Nei's standard genetic distance between the breeds studied showed that three Iranian goat breeds (Mahabadi, Lori and Markhoz) were clustered closer together, while the Beetal breed formed a separate cluster. In the constructed dendrogram of the subpopulations, the S and T subpopulations of each breed were clustered together. The constructed dendrogram of the Beetal breed and the S and T subpopulations of all breeds studied showed a separate cluster for the Beetal breed as an imported breed and another cluster for the S and T subpopulations as Iranian native breeds. The current study showed that the ISSR markers studied had no potential to genetically separate S and T subpopulations. On the other hand, these ISSR markers can be used for the clustering of distinct populations.

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Genetic Diversity Analysis of the Gohilwari Breed of Indian Goat (Capra hircus) Using Microsatellite Markers

Cited by 38 publications

References 35 publications

Morphological and molecular genetic diversity of Syrian indigenous goat populations

Morphological and molecular genetic diversity of Syrian indigenous goat populations

Genetic diversity and bottleneck analysis of Konkan Kanyal goats

Genetic diversity and distance of Iranian goat breeds (Markhoz, Mahabadi and Lori) compared to the Beetal breed using inter-simple sequence repeat (ISSR) markers

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