Genetic diversity analysis of the Uruguayan Creole cattle breed using microsatellites and mtDNA markers

Abstract

ABSTRACT. The Uruguayan Creole cattle population (N = 600) is located in a native habitat in south-east Uruguay. We analyzed its genetic diversity and compared it to other populations of American Creole cattle. A random sample of 64 animals was genotyped for a set of 17 microsatellite loci, and the D-loop hyper-variable region of mtDNA was sequenced for 28 calves of the same generation. We identified an average of 5.59 alleles per locus, with expected heterozygosities between 0.466 and 0.850 and an expected mean heterozygosity of 0.664. The polymorphic information content ranged from 0.360 to 0.820, and the global F IS index was 0.037. The D-loop analysis revealed three haplotypes (UY1, UY2 and UY3), belonging to the European matriline group, with a haplotype diversity of 0.532. The history of the population, changes in the effective population size, bottlenecks, and genetic drift are possible causes of the genetic variability patterns that we detected.

DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis

“…The cattle introduced at the beginning of the 17th century multiplied immediately: thousands first, multitudes ("millions"?) later, becoming semi-wild and roaming over the territory known later as the Banda Oriental of the River Uruguay [ 98 ]. Thus, "gaucho" horsemen culture and pastoral life became the way of life for the European colonizers that would later populate the region.…”

The complete mitochondrial genome of Uruguayan native cattle (Bos taurus)

“…At the same time, identification of beef types becomes important for importing countries and consumers. Based on gene sequence, random amplified polymorphic DNA (RAPD) and microsatellite (MS) markers have been used to evaluate poolspecific products which are quite valuable (Rinc on et al 2000;Armstrong et al 2013). However, no available genetic sequence data of the cattle breed in Uruguay restricts the development of detection methods.…”

A new high-frequency allele of the BM2113 locus in the Yunnan mithun population

“…Liao et al defined it as specific-allele 120 bp (63.2%) in mithun from Yunnan Province (Liao et al, 2008;Zhang et al, 2008); however, there were distinct discrepancies between this report and that from Tian et al (2011), who used silver staining. Allele 121 bp was absent in European cattle breeds (MacHugh et al, 1997), Brazilian Gir (Bicalho et al, 2006), Vietnamese cattle and wild gaur (Nguyen et al, 2007), Indian Kangayam cattle (Karthickeyan et al, 2009), Colombia Brahman cattle (Novoa and Usaquén, 2010), Uruguayan Creole cattle (Armstrong et al, 2013), Mongolian and Russian yak (Xuebin et al, 2005), Swiss yak (Nguyen et al, 2005), and Chinese yak (Liao et al, 2008;Zhang et al, 2008). No detailed data were shown for other bovid species in the previous studies on the BM2113 locus (Kantanen et al, 2000;Ritz et al, 2000;Kim et al, 2002;Zhang et al, 2007aZhang et al, ,b, 2011Mao et al, 2008;Wang et al, 2008;Rivière-Dobigny et al, 2009;Li and Kantanen, 2010;Azam et al, 2012).…”