Genetic diversity analysis of Linseed (Linum usitatissimum L.) accessions using RAPD Markers

Abstract

It is important to analyse the degree of genetic variation existing within the genome to extend the genetic base of linseed/flaxseed accessions in order to preserve, evaluate and use genetic resources accurately and successfully. The main aim of the current investigation was to evaluate the scope and spread of genomic variation across different linseed accessions by employing molecular markers (RAPD). The genomic DNA of 12 linseed accessions was amplified with 16 decamer RAPD primers that generated 81 total bands, among which 75 bands were polymorphic and 6 bands were monomorphic. Polymorphic band numbers varied from least 2 (OPS-11) to highest 10 (OPS-07). The magnitude of polymorphism ranged from 75% to 100% among all accessions with a mean of 93.15 % across all the accessions. The value of Polymorphic Information Content (PIC) varied from 0.133% to 0.708% with a mean of 0.45% for each primer. The maximum PIC value (0.708) was found with the primer OPS-07 and (0.702) with OPM-13. The primer OPS-03 showed the minimum PIC value (0.133). Two main different clusters -I and -II were seen in the cluster analysis depending on RAPD data. Cluster-II comprises one accession (IC 564585) that was the highly varied accession, whereas Cluster-I comprises of some sub clusters with all the remaining accessions. The Jaccard's similarity coefficient varied from 8.2 to 96.3%. The accessions BHU-A and BHU-B had the highest genetic similarity (96.3%), followed by BHU-B and IC 564605 (96.2%). More divergent accessions were discovered to be IC 564585, IC 564616, IC 564631, IC 564622, and IC 564630. The current investigation provides innovative knowledge to breeders on the germplasm of linseed that would be employed in subsequent research to improve linseed genotypes.

Використання Rapd-Аналізу Для Вивчення Генетичного Поліморфізму Сортів Льону Української Та Зарубіжної Селекції

“…З метою дослідження генетичної варіабельності зародкової плазми насіння льону, було використано 12 зразків індійського походження та 16 декамерних RAPD-праймерів (Nagabhushanam et al, 2021). У результаті досліджень було встановлено надзвичайно високий рівень генетичного поліморфізму -від 75 до 100% і виявлено доволі тісну спорідненість між зразками.…”

Comparative analysis of physico-chemical properties and fatty acid composition of linseed (Linum usitatissimum L.) oils of Indian accessions

“…Out of 12 linseed accessions, five accessions BHU-A, BHU-B, IC-564585, IC 564616, and IC 564660 were selected based on their high content of ALA and highest genetic diversity [27] to carry out physicochemical parameters such as SV, Iodine value, peroxide value, acid value, refractive index, and viscosity.…”

Molecular genetic diversity of flax cultivars (<i>Linum usitatissimum</i> l.) represented in the State Register for Selection Achievements of the Russian Federation

“…Оставшаяся часть -костра, являющаяся побочным продуктом -применяется для получения энергии в строи тельной и других отраслях промышленности. Масло, получаемое из семян масличного льна, в зависимости от жирнокислотного состава, применяется в пищевой, фармацевтической и химической промышленности (Poro kho vinova et al, 2019;Nagabhushanam et al, 2021;Saroha et al, 2021).…”