Genetic diversity analysis of Leuconostoc mesenteroides from Korean vegetables and food products by multilocus sequence typing

Abstract

In the present study, 35 Leuconostoc mesenteroides strains isolated from vegetables and food products from South Korea were studied by multilocus sequence typing (MLST) of seven housekeeping genes (atpA, groEL, gyrB, pheS, pyrG, rpoA, and uvrC). The fragment sizes of the seven amplified housekeeping genes ranged in length from 366 to 1414 bp. Sequence analysis indicated 27 different sequence types (STs) with 25 of them being represented by a single strain indicating high genetic diversity, whereas the remaining 2 were characterized by five strains each. In total, 220 polymorphic nucleotide sites were detected among seven housekeeping genes. The phylogenetic analysis based on the STs of the seven loci indicated that the 35 strains belonged to two major groups, A (28 strains) and B (7 strains). Split decomposition analysis showed that intraspecies recombination played a role in generating diversity among strains. The minimum spanning tree showed that the evolution of the STs was not correlated with food source. This study signifies that the multilocus sequence typing is a valuable tool to access the genetic diversity among L. mesenteroides strains from South Korea and can be used further to monitor the evolutionary changes.

Tracking of Intentionally Inoculated Lactic Acid Bacteria Strains in Yogurt and Probiotic Powder

“…The same set of gene sequences were utilized for the comparative gene sequence analysis to identify the deliberately inoculated Leu. mesenteroides 11251 and L. brevis B151 in yogurt [45,46]. For the L. plantarum strains, the housekeeping genes were used from an earlier described MLST study [25].…”

Analysis of rpoB polymorphism and PCR-based approaches for the identification of Leuconostoc mesenteroides at the species and subspecies level

“…Over time, several molecular techniques, including RAPD-PCR, rep-PCR, species-specific PCR, multiplex-PCR, 16S PCR-RFLP, ARDRA, MLST and partial sequencing of housekeeping genes (Frantzen et al, 2017;Gu et al, 2012;Rahkila et al, 2014;Sharma et al, 2018), have been proposed to identify the Leuc. mesenteroides strains at species and/or subspecies level, with variable degree of success due to some practical limitations (poor reproducibility, low discriminative power).…”

Analysis of Leuconostoc citreum strains using multilocus sequence typing

“…Therefore, genotypic methods are playing a significant role in phylogenetic classification and identification of the bacterial species. Till date, many molecular methods have been used for the typing of Leuconostoc genus like restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD)-PCR, amplified fragment length polymorphism (AFLP), pulsed-field gel electrophoresis (PFGE), repetitive element palindromic PCR (rep-PCR) using different primers, and multilocus sequence typing (MLST) (Sharma et al, 2018). MLST is based on the principles of multilocus enzyme electrophoresis (MLEE) (Maiden et al, 1998).…”

“…Initially, the technique was described in 1998 for the identification of virulent strains of Neisseria meningitides (Maiden et al, 1998). Continuously, several MLST schemes have been created for bacterial species including the LAB (Sharma et al, 2018). Identification of industrially important bacterial strain is essential in both basic and applied research.…”