Genetic diversity analysis of Croton antisyphiliticus Mart. using AFLP molecular markers

Oliveira, Tayná; Pereira, Adriana; Coppede, Juliana S.; França, Suzelei de Castro; Ming, Lin Chau; Bertoni, Bianca Waléria

doi:10.4238/gmr.15017461

Cited by 10 publications

(7 citation statements)

References 21 publications

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“…Despite the great medicinal importance, genetic studies that are essential for the development of conservation and management strategies are still scarce in the genus with few species having been analyzed, such as C. antisyphiliticus Mart. (Oliveira et al 2016), C. cajucara Benth. (Angelo et al 2005), C. floribundus (Silvestrini et al 2013(Silvestrini et al , 2015, C. heliotropiifolius Kunth ( de Oliveira Rocha et al 2016), C. linearifolius Mull.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of Croton urucurana Baill. (Euphorbiaceae) in Central Brazil by ISSR markers

et al. 2020

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Studies on the genetic diversity of species with pharmacological potential are essential for the conservation of genetic resources. The genetic characterization of a species can guide efforts to collect and conserve germplasm and, in the future, genetic improvement. Domestic cultivation of medicinal plants is an important strategy for protecting these species from over harvesting. Genetic studies of medicinal species by molecular markers could allow for assessment and management of these species. Croton urucurana Baill. is widely used in Brazilian traditional medicine, but there are practically no data about its genetic patterns. We used 12 ISSR markers to evaluate the genetic diversity levels of 30 trees distributed in three populations of C. urucurana, two of which were located in Brazilian savanna (Cerrado) and one on semi-deciduous tropical forest areas. From a total of 82 bands recorded, 89% were polymorphic and presented a Shannon's diversity index of 0.26. The populations formed two distinct groups: Two populations were divided each into a different genetic group, while the Aquidauana population, which is in a transition area between the Cerrado and the Pantanal, presented a mixture of the two genetic groups. Croton urucurana has G ST values (0.200) similar to those found in species with a mixed mating system, but the lack of detailed studies on the reproductive system of this species makes it difficult to correctly interpret the origin of the population structure observed. Moreover, even using a simpler marker we were able to identify regions that deserve further studies, especially from a chemical point of view, to see whether there are any differences between the populations that could help in defining future conservation strategies, management and genetic improvement plans of the species.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of Croton urucurana Baill. (Euphorbiaceae) in Central Brazil by ISSR markers

et al. 2020

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“…Concerning literature, there is greater difficulty in obtaining intact DNA sampling from tropical species, due to the presence of secondary compounds, especially of the phenol class (Rath et al, 1998; Deshmukh et al, 2007). During genetic material extraction in Croton antisyphiliticus, Oliveira et al (2016) observed viscosity and oxidation in the samples. Scaldaferri et al (2013), evaluating different extraction protocols of Croton linearifolius, observed the presence of polysaccharides in samples and found that methods using CTAB were the most efficient.…”

Section: Resultsmentioning

confidence: 97%

“…Because of this great medicinal importance, genetic studies are essential to develop conservation strategies and also products (Alves et al, 2012). For Croton genus there are some research related to genetic diversity (Angelo et al, 2005;Silvestrini et al, 2015;Rinthong et al, 2011;Scaldaferri, 2013Scaldaferri, , 2014Oliveira et al, 2016;Almeida-Pereira et al, 2017;Silva et al, 2018), however, molecular information are still scarce; for species C. urucurana they are almost non-existent and it is necessary to advance in the genetic diversity characterization of this species.…”

Section: Introductionmentioning

confidence: 99%

Transferability of microsatellite loci from Croton floribundus Spreng. to Croton urucurana Baill. (Euphorbiaceae)

Motta¹,

Skowronski²,

Velasques³

et al. 2019

Afr. J. Biotechnol.

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Croton urucurana, known as "sangra-d'água", is a tree species with timber and beekeeping potential, and also with medicinal potential. The use of microsatellite markers, simple sequence repeats (SSRs) is efficient in population genetic studies, with high transferability rate between correlated species. The present study aimed to optimize the polymerase chain reaction (PCR) protocol and to test the transferability of seven primers developed for Croton floribundus to C. urucurana. The amplification of 113 accessions from different Brazilian biomes (Cerrado, Pantanal and Mata Atlântica) was tested. For optimization, the concentration and label of the MgCl 2 reagent was varied in addition to annealing temperature. Four tested primers showed amplification products, guaranteeing 57% of transferability. This result confirms the efficiency of C. floribundus microsatellite loci for future studies of genetic variability in C. urucurana.

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“…A técnica AFLP foi empregada para avaliar a variabilidade genética em populações naturais de C. antisyphiliticus, espécie utilizada no tratamento de infecções do aparelho urogenital, presentes nos estados de Minas Gerais, São Paulo e Goiás (OLIVEIRA et al, 2016). Além das características inerentes aos marcadores dominantes, os marcadores AFLP apresentam como desvantagens a exigência de uma maior quantidade de DNA, várias etapas e reagentes que aumentam o custo da técnica (TURCHETTO-ZOLET et al, 2017), sendo estes fatores potenciais/prováveis motivos para o reduzido número de estudos de Croton com estes marcadores, se comparado com os RAPD e ISSR (quadro 1).…”

Section: Resultsunclassified

CONTRIBUIÇÕES DA BIOLOGIA MOLECULAR PARA CARACTERIZAÇÃO, CONSERVAÇÃO E USO DO GÊNERO Croton L. (Euphorbiaceae)

Silva¹,

Freitas²,

Santos³

et al. 2019

Enci Bio

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The genetic-molecular knowledge of the species that present relevance as a natural resource is fundamental for the elaboration of strategies of conservation and sustainable use of biodiversity. Molecular markers are important tools for obtaining relative genetic data from population estimates to identification of genomic regions associated with phenotypic characteristics. The aim of this study was to present an overview of the available knowledge in the literature on the application of molecular markers for the study of species of the genus Croton, indicating both the main gaps and perspectives regarding the available molecular tools and its application in the understanding of the genus, aiming at the conservation and use of these species. For that, searches were made on databases (indexers) of scientific publications. In summary, genetic studies with the application of molecular markers were restricted to seven species of Croton spp., corresponding to less than 1% of the species of the genus, and most dominant molecular markers were used, a fact that limits the possibility of authors to broaden their discussions and conclusions. The data presented indicate the lack of knowledge regarding the genetic characteristics and the population genetic structure for Croton spp.. Consequently, this reality testifies to a set of opportunities for conducting new research that contributes to the characterization and use of this biodiversity.

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Genetic diversity analysis of Croton antisyphiliticus Mart. using AFLP molecular markers

Cited by 10 publications

References 21 publications

Genetic diversity and population structure of Croton urucurana Baill. (Euphorbiaceae) in Central Brazil by ISSR markers

Genetic diversity and population structure of Croton urucurana Baill. (Euphorbiaceae) in Central Brazil by ISSR markers

Transferability of microsatellite loci from Croton floribundus Spreng. to Croton urucurana Baill. (Euphorbiaceae)

CONTRIBUIÇÕES DA BIOLOGIA MOLECULAR PARA CARACTERIZAÇÃO, CONSERVAÇÃO E USO DO GÊNERO Croton L. (Euphorbiaceae)

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