Genetic Diversity Analysis of Common Bean (Phaseolus Vulgaris L.) Genotypes Using Scot Polymorphism

Abstract

Common bean (Phaseolus vulgaris L.) is legume crop of worldwide importance and due to optimal content of proteins and other essential compounds it has a potential as a functional food. Genetic diversity studies are significant from the point of obtaining information important for breeding process. The goal of present work was to analyze genetic diversity among 34 genotypes of common bean from different countries using 5 SCoT (Start Codon Targeted) markers. Altogether 82 DNA fragments were amplified, out of which 66 fragments were polymorphic with an average of 11 polymorphic fragments per primer. The highest number of polymorphic fragments was detected by marker SCoT 59 (15). The percentage of polymorphism ranged from 57.17% (SCoT 2) to 78.57% (SCoT 19) with an average of 67.3%. PIC values varied from 0.719 (SCoT 19) to 0.886 (SCoT 3) and average PIC value was 0.802. The DI values varied between 0.722 (SCoT 19) and 0.888 (SCoT3), with an average of 0.804. The dendrogram of 34 genotypes of common bean, constructed based on hierarchical cluster analysis separated genotypes into two clusters (I and II). Cluster I was formed by two subclusters. Thirty-three genotypes were included in cluster I and genotype Albena (Slovak Republic), which significantly differed from other genotypes in seed size was included in cluster II. Obtained results support the effectiveness of SCoT markers in the analysis of common bean useful for genotypes differentiating and assessment of genetic diversity in the set of common bean germplasm.

Efficiency of RAPD and SCoT Markers in the Genetic Diversity Assessment of the Common Bean

“…Based on the results, they considered using SCoT markers as a suitable technique for the genetic identification of genetic material based on its geographic origin. The sufficiency of SCoT markers in the genetic studies is also supported by the results presented by [30], who used five SCoT makers for the differentiation and genetic diversity study of 34 bean genotypes.…”

“…The discovery of genetic relationships among plant genotypes based on DNA polymorphism may contribute to important knowledge leading not only to the simplification of the selection of germplasm for the breeding process but also to efficient conservation strategies [37,38] of genetic resources in gene banks. Many types of developed molecular markers have been used for the detection of genetic diversity in plants [30,31,[39][40][41][42][43][44][45].…”

“…In the different marker systems, numerous Polymerase Chain Reaction (PCR)-based markers were proven to be efficient for the investigation of genetic variability in collections of plant species [18], including the common bean. Many molecular markers, including RAPD [19][20][21], AFLP [22][23][24], ISSR [25,26], SSR [27][28][29], and SCoT [30,31], were successfully applied in the molecular variability analyses of the common bean. Random Amplified Polymorphic DNA (RAPD) markers are one of the types of arbitrarily amplified dominant markers.…”