Genetic diversity analysis of Blastocystis subtypes and their distribution among the domestic animals and pigeons in northwest of Iran

Rostami, Mehrdad; Harandi, Majid Fasihi; Salehi, Reza; Aspatwar, Ashok; Derakhshan, Fatemeh Khadivi; Raeghi, Saber

doi:10.1016/j.meegid.2020.104591

Cited by 21 publications

(6 citation statements)

References 29 publications

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“…This high rate of Blastocystis sp. colonization in France was roughly similar to those observed using molecular methods in bovid from other countries, including Lebanon (63.4%) [22], Japan (54.1%) [23], Thailand (50.0%) [24], Colombia (80.0%) [25], Libya (41.7%) [11], Malaysia (34.5%) [26], Iran (33.3 and 35%) [27,28], and Spain (32.1%) [13]. On the other hand, it was significantly higher than the ones reported in Korea (6.7%) [29], China (9.5%, 10.3% and 27.9%) [30][31][32], Iran (9.6%) [33], Turkey (11.25%) [34], Brazil (21.4%) [35], the UK (22.6%) [11], United Arab Emirates (22.7%) [36], the United States (19.1% and 2.9%) [37,38], and Malaysia (25% and 25.4%) [39,40].…”

Section: Resultssupporting

confidence: 77%

Animal, Herd and Feed Characteristics Associated with Blastocystis Prevalence and Molecular Diversity in Dairy Cattle from the North of France

et al. 2022

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Despite the major impact of Blastocystis sp. in terms of prevalence in human and animal populations and the risk of zoonotic transmission, no epidemiological survey has yet been conducted in cattle herds in France. The aim of this study was thus to assess the prevalence and molecular diversity of Blastocystis sp. and associated factors in dairy cattle from the north of France. A total of 1581 fecal samples were collected from 1246 animals reared in 20 farms. Molecular detection of the protozoan was performed by real-time PCR and indicated an overall prevalence of Blastocystis sp. Reaching 54.8% in the study population. Important inter-herd variation (from 22.2% to 76.5%) of Blastocystis sp. Prevalence was also reported. Sequence analysis of 159 positive samples highlighted a very large predominance of ST10 (36/159) and ST14 (64/159), and ST2 was only found in 2 samples. Mixed subtype infections were common, representing 35.8% of sequenced samples (57/159). A putative correlation between Blastocystis sp. colonization and various animal and herd characteristics or feed intake was subsequently investigated. The protozoan was less prevalent in cows that have recently calved but Blastocystis sp. carriage was not significantly related to age. Blastocystis sp. colonization also decreased with high beet pulp and pasture grass consumption and increased with corn silage intake. Finally, the only significant association between Blastocystis sp. STs and animal and herd characteristics was the number of lactations of cows, with a predominance of ST14 in cows that calved once only.

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Section: Resultssupporting

confidence: 77%

Animal, Herd and Feed Characteristics Associated with Blastocystis Prevalence and Molecular Diversity in Dairy Cattle from the North of France

et al. 2022

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“…The overall infection rate of Blastocystis in Tibetan antelope was 4.8% (30/627) in Tibet, which was lower than the prevalence of 5.5% (6/109) and 6.0% (50/832) identified in sheep ( Wang et al., 2018a ; Li et al., 2018 ) in China and 19.3% (9/150) and 32.0% (32/100) in sheep in Iran ( Rostami et al., 2020 ; Salehi et al., 2021 ). The difference in the prevalence of Blastocystis may be related to the living environment and geographical factors of different countries ( Tan, 2008 ).…”

Section: Discussionmentioning

confidence: 72%

The Presence of Blastocystis in Tibetan Antelope (Pantholops hodgsonii)

Geng

Sun

Jiang

et al. 2021

Front. Cell. Infect. Microbiol.

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Blastocystis is a protozoan that parasitizes the intestines. A number of hosts of Blastocystis have been found, including human and animals. However, there has been no research on the prevalence of Blastocystis in Tibetan antelope. Here, a molecular test was performed using 627 Tibetan antelope fecal samples collected on Tibet in China from 2019 to 2020. The result showed that 30 (4.8%) samples were Blastocystis positive. The highest prevalence of Blastocystis was in Shuanghu County (25/209, 12.0%), followed by Shenza County (2/103, 1.9%), Nyima County (3/182, 1.6%), and Baigoin County (0/133, 0.0%). In addition, logistic regression analysis showed that the gender, sampling year, and area of Tibetan antelope were risk factors for Blastocystis prevalence. Three subtypes (ST10, ST13, and ST14) of Blastocystis were found in Tibetan antelope through a subtype sequence analysis, and ST13 was identified to be the dominant subtype. This is the first investigation for the infection of Blastocystis in Tibetan antelope. Collectively, the data in this study have expanded the host range of Blastocystis and provided basic information for the distribution of Blastocystis subtypes, which could support the prevention of Blastocystis infection in wild animals.

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“…In the present study, the Blastocystis prevalence in sheep and pigs in Shanxi province were 16.26% (80/492) and 14.09% (51/362), respectively. The Blastocystis prevalence in sheep in Shanxi province was lower than that in Turkey (38.2%, 84/220) [ 31 ], the United Arab Emirates (63.6%, 7/11) [ 32 ], Italy (81.8%, 9/11) [ 33 ], Brazil (33.3%, 1/3) [ 34 ], Iran (20.9%, 14/67) [ 35 ], and some provinces of China, such as Jiangsu province (24.00%, 18/75) and Shandong province (16.67%, 10/60) [ 36 ]. However, the 16.26% Blastocystis prevalence in sheep in Shanxi province was higher than that in Anhui province (6.4%, 22/697) and the Qinghai-Tibetan Plateau (8.55%, 53/620) [ 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Genetic Characterization of Blastocystis in Sheep and Pigs in Shanxi Province, North China: From a Public Health Perspective

Wei,

Qin,

Zhang

et al. 2023

Animals

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Blastocystis is a common zoonotic intestinal protozoan and causes a series of gastrointestinal symptoms in humans and animals via the fecal–oral route, causing economic losses and posing public health problems. At present, the prevalence and genetic structure of Blastocystis in sheep and pigs in Shanxi province remains unknown. Thus, the present study collected 492 sheep fecal samples and 362 pig fecal samples from three representative counties in northern, central and southern Shanxi province for the detection of Blastocystis based on its SSU rRNA gene. The results showed that the overall prevalence of Blastocystis in the examined sheep and pigs were 16.26% and 14.09%, respectively. Sequences analyses showed that four known subtypes (ST5, ST10, ST14 and ST30) in sheep and two subtypes (ST1 and ST5) in pigs were detected in this study, with ST5 being the predominate subtype among the study areas. Phylogenetic analysis showed that the same subtypes were clustered into the same branch. This study reveals that sheep and pigs in Shanxi province are hosts for multiple Blastocystis subtypes, including the zoonotic subtypes (ST1 and ST5), posing a risk to public health. Baseline epidemiological data are provided that help in improving our understanding of the role of zoonotic subtypes in Blastocystis transmission.

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Genetic diversity analysis of Blastocystis subtypes and their distribution among the domestic animals and pigeons in northwest of Iran

Cited by 21 publications

References 29 publications

Animal, Herd and Feed Characteristics Associated with Blastocystis Prevalence and Molecular Diversity in Dairy Cattle from the North of France

Animal, Herd and Feed Characteristics Associated with Blastocystis Prevalence and Molecular Diversity in Dairy Cattle from the North of France

The Presence of Blastocystis in Tibetan Antelope (Pantholops hodgsonii)

Prevalence and Genetic Characterization of Blastocystis in Sheep and Pigs in Shanxi Province, North China: From a Public Health Perspective

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