Genetic Diversity Analysis in Sunflower (Helianthus annuus L.)  parental Lines Using SSR and RAPD Markers

Suresha, P. G.; Kulkarni, Vikas; Supriya, S.M.; Darshan, S.; Patil, Chandrashekar B.

doi:10.20546/ijcmas.2017.607.244

Cited by 8 publications

(6 citation statements)

References 8 publications

(5 reference statements)

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“…Various previous researchers who used SSR markers for sunflower characterization at molecular data also found that sunflower lines had a clear tendency of grouping restorer and CMS lines into different clusters. For examining the genetic diversity in sunflower SSR, markers are a powerful as it reveals high level of polymorphism among sunflower lines (Lochner, 2011 ; Zia et al, 2014 ; Suresha et al, 2017 ; Ijaz et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular markers-based DNA fingerprinting coupled with morphological diversity analysis for prediction of heterotic grouping in sunflower (Helianthus annuus L.)

et al. 2022

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Cultivated sunflower holds a very narrow genetic base and the efficient utilization of available genetic diversity is very important for a successful breeding program. In the present study, 109 sunflower genotypes were assessed for diversity paneling through a combined approach of morphological and molecular markers analysis. Morphological parameters including days to flower initiation, days to flower completion, plant height, stem curvature, number of leaves per plant, leaf area, head diameter, hundred seed weight, and seed yield per plant were studied. Simple sequence repeats (40 DNA markers) were deployed for diversity profiling. Data were analyzed by both univariate and multivariate statistics. SD and coefficient of variation confirm the presence of significant amounts of genetic variation for all the morphological parameters. Cluster Analysis and Principal Component Analysis further confirm the presence of distinct grouping patterns in the studied material. Cluster analysis of both morphological and molecular analysis revealed that restorer lines tend to group separately from A, B, and open-pollinated lines. Further grouping, at the sub-cluster level, revealed six distinct sub-clusters in each of the two major clusters. In total, 12 genotypes, 6 CMS lines (CMS-HAP-12, CMS-HAP-54, CMS-HAP-56, CMS-HAP-99, CMS-HAP-111, and CMS-HAP-112) and 6 restorer lines (RHP-38, RHP-41, RHP-53, RHP-68, RHP-69, and RHP-71) could be used as potential parents for hybrid development. As genotypes of similar genetic backgrounds tend to group closer, it is deduced that one genotype with the highest seed yield per plant could be used for further hybrid breeding programs in sunflowers.

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Section: Discussionmentioning

confidence: 99%

Molecular markers-based DNA fingerprinting coupled with morphological diversity analysis for prediction of heterotic grouping in sunflower (Helianthus annuus L.)

et al. 2022

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“…DNA markers viz., RFLP, RAPD, SSR, ISSR etc., can be used to assess the diversity studies. Among them, SSR markers have great potential in genetic and breeding studies (Shoba et al, 2010;Ramanaiah and Kadirvel, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, molecular method of diversity research has been conducted in most of oilseed crops by SSR markers (Ramanaiah and Kadirvel, 2021;Yihan et al, 2022;Ahmed et al, 2022in sunflower, Teklu et al,2022 in sesame, Tomar et al, 2022 in groundnut andKumar et al, 2022 in soybean) due to its codominant and highly reproducible characteristics. The relationship between molecular diversity with hybrid performance and heterosis was studied in several crops to access the reliability of markers-based prediction in hybrid performance (Xiao et al, 1996;Sureja et al, 2006;Gupta et al, 2018 andSomashekhar et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Association between molecular diversity and hybrid performance in sunflower (Helianthus annuus L.)

2023

EJPB

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The present study was aimed to assess the molecular genetic diversity in 14 parental lines (10 maintainers and 4 restorers) of sunflower crop using 51 SSR markers. A total of 147 alleles were produced with the range of 1-6 alleles per locus. The polymorphic information content (PIC) values ranged from 0.12(ORS1198 in LG5) to 0.74(ORS384 in LG15) with an average PIC of 0.51. A phylogenetic tree was constructed by neighbour-joining method which grouped the parents into four clusters. Among the maintainer and restorer combinations, the dissimilarity index was maximum between CMS 519B and CSFI 99 (0.67) which indicateed high divergence between them. Lower dissimilarity value was observed between RCR CMS 38B and LTRR 341 (0.40) suggested less divergence between them. Mahalanobis D 2 analysis was also carried out among the parental lines. The D 2 analysis grouped the parental lines into six clusters. Among them genotype pairs CMS104B vs RHA 1-1 and CSFI 99 vs LTRR341 had highest and lowest D 2 values respectively. Dissimilarity and D 2 values for parental combinations, mean performance, mid and better parental heterosis of 40 hybrids for yield and its component traits were subjected to simple correlation analysis. The D 2 analysis did not show any association with performance of hybrids. However, the dissimilarity values showed association with hybrid performance and heterosis for plant height and volume weight. Hence, the present study indicated that the selection of parental genotypes with high molecular diversity could result in improved performance of plant height and volume weight in sunflower hybrids. However further study is required with multi environment data sets.

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“…In this study, randomly amplified polymorphic DNA (RAPD) were used because this analysis is commonly used for studying taxonomy of various organisms (Suresha, 2017). An RAPD assay is rapid and easy to perform and also requires only small amount of DNA.…”

Section: Introductionmentioning

confidence: 99%

Molecular Phylogeny of Sunflower Cultivars of Teddy Bear, Skyscraper, Lemon Queen and Common Sunflower Using RAPD Markers

Tanudharma

Sasongko²,

Yuniaty³

2020

bioe

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Helianthus or sunflower is a genus of plant comprising about 70 species. Common sunflower and other members of Helianthae are cultivated in temperate regions and some tropical regions as food crops for humans, cattle, poultry, and as ornamental plants. The common sunflower is valuable with respect of economic and ornamental point of view. There are many cultivars of sunflower including teddy bear, skyscraper, and lemon queen. Variation among these cultivars has been studied using molecular techniques and the result were used to develop the phylogeny among them. Random Amplified Polymorphic DNA (RAPD) is one of molecular techniques that were used for this purpose. The purpose of this study was to contruct the phylogeny of three sunflower cultivars and common sunflower based on RAPD markers. The RAPD primers used in this study were OPA-2, OPA-9, OPA-13, OPB-2, OPB-4, OPB5, OPB-7, and OPB-11. Data analysis based on molecular data showed that genetic relationship among Lemon Queen, Skyscraper, Teddy Bear and Common sunflower based on RAPD markers shows that the cultivars studied are grouped into three main groups, namely: Group I Lemon Queen and Skyscraper, Group II Teddy Bear, and Group III Common sunflower; the closest kinship is shown between Lemon Queen and Skyscraper.

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Genetic Diversity Analysis in Sunflower (Helianthus annuus L.) parental Lines Using SSR and RAPD Markers

Cited by 8 publications

References 8 publications

Molecular markers-based DNA fingerprinting coupled with morphological diversity analysis for prediction of heterotic grouping in sunflower (Helianthus annuus L.)

Molecular markers-based DNA fingerprinting coupled with morphological diversity analysis for prediction of heterotic grouping in sunflower (Helianthus annuus L.)

Association between molecular diversity and hybrid performance in sunflower (Helianthus annuus L.)

Molecular Phylogeny of Sunflower Cultivars of Teddy Bear, Skyscraper, Lemon Queen and Common Sunflower Using RAPD Markers

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