Genetic diversity analysis in Plantago ovata and some of its wild allies using RAPD markers

Kour, Balbir; Kotwal, Shivanjali; Dhar, Manoj K.; Kaul, Sanjana

doi:10.3103/s1068367416010055

Cited by 5 publications

(6 citation statements)

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“…Continued studies have extended our knowledge to distinguish and understand the genomic structure of and phylogenetic relationships among Plantago species 23,74,75 . Taxonomy and phylogeny of the Plantago genus within Lamiales have been widely examined at the genus level 20,22 . Previous evolutionary relationships among different Plantago species were estimated by analysing nuclear polymorphisms, random amplified polymorphic DNA (RAPD) profiles, chloroplast DNA restriction fragment-length polymorphisms (RFLP), SSR region, and ITS region as well as the sequences of 5 S rRNA genes, rps14 gene, and plastid trnL-F 20,22,75,76 .…”

Section: Discussionmentioning

confidence: 99%

“…Taxonomy and phylogeny of the Plantago genus within Lamiales have been widely examined at the genus level 20,22 . Previous evolutionary relationships among different Plantago species were estimated by analysing nuclear polymorphisms, random amplified polymorphic DNA (RAPD) profiles, chloroplast DNA restriction fragment-length polymorphisms (RFLP), SSR region, and ITS region as well as the sequences of 5 S rRNA genes, rps14 gene, and plastid trnL-F 20,22,75,76 . However, complete genome sequencing provides more detailed insight 31,77,78 .…”

Section: Discussionmentioning

confidence: 99%

“…The genetic diversity of Plantago species is important for breeding various cultivars, which are valuable additions that are resistant to different diseases 19 . The meiotic system and chromosomal structure of P. ovata have various features which account for its narrow genetic base such as a small 621-Mb genome size with 4 (2n = 2x = 8) chromosomes 20 .…”

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confidence: 99%

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Expanded inverted repeat region with large scale inversion in the first complete plastid genome sequence of Plantago ovata

Asaf

Khan

Lubna

et al. 2020

Sci Rep

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Plantago ovata (plantaginaceae) is an economically and medicinally important species, however, least is known about its genomics and evolution. Here, we report the first complete plastome genome of P. ovata and comparison with previously published genomes of related species from plantaginaceae. the results revealed that P. ovata plastome size was 162,116 bp and that it had typical quadripartite structure containing a large single copy region of 82,084 bp and small single copy region of 5,272 bp. The genome has a markedly higher inverted repeat (IR) size of 37.4 kb, suggesting large-scale inversion of 13.8 kb within the expanded IR regions. In addition, the P. ovata plastome contains 149 different genes, including 43 tRNA, 8 rRNA, and 98 protein-coding genes. The analysis revealed 139 microsatellites, of which 71 were in the non-coding regions. Approximately 32 forward, 34 tandem, and 17 palindromic repeats were detected. The complete genome sequences, 72 shared genes, matK gene, and rbcL gene from related species generated the same phylogenetic signals, and phylogenetic analysis revealed that P. ovata formed a single clade with P. maritima and P. media. the divergence time estimation as employed in BeASt revealed that P. ovata diverged from P. maritima and P. media about 11.0 million years ago (Mya; 95% highest posterior density, 10.06-12.25 Mya). In conclusion, P. ovata had significant variation in the IR region, suggesting a more stable P. ovata plastome genome than that of other plantaginaceae species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Expanded inverted repeat region with large scale inversion in the first complete plastid genome sequence of Plantago ovata

Asaf

Khan

Lubna

et al. 2020

Sci Rep

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“…Molecular markers, such as RAPDs, are powerful in analyzing genetic relation and diversity, and evaluating taxonomic identity of plants (Colling et al, 2010;Kocsis et al, 2015;Kour et al, 2016;Wu et al, 2005). The cluster analysis based on RAPD markers revealed a distant genetic relationship between the two sections of Narcissus, whereas a close relationship within individual section, sharing agreement with previous studies (Chen et al, 2002(Chen et al, , 2003Dong, 2012 (Lu et al, 2007) and suggest these ecotypes should be same on DNA level.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, Hodgins and Barrett (2007) investigated cpDNA sequence and nuclear microsatellite variation among populations of the wild daffodil (Narcissus triandrus). More recently, RAPD technique has gained its central position for genetic diversity studies due to its low expense and high efficiency (Ambade et al, 2015;Bradakci, 2001;Kocsis et al, 2015;Kour et al, 2016). In daffodils, RAPD was once applied to analyze their germplasm resources, variety identifications (Chen et al, 2002(Chen et al, , 2003Dong, 2012;Wu et al, 2005;Zhu, 2003), and population genetic structures (Colling et al, 2010).…”

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confidence: 99%

Genetic Diversity Analysis of Nine Narcissus Based on Morphological Characteristics and Random Amplified Polymorphic DNA Markers

Liu

Zhang

Shi

et al. 2017

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Genetic diversity of Narcissus was systematically studied on both morphological and molecular levels. Twenty-four characteristics of nine narcissi were observed and their differences evaluated by clustering method. The results showed that nine narcissi can be divided into two subclusters: one comprised by Narcissus pseudonarcissus, the other by Chinese Narcissus. The morphological diversity among five cultivars of N. pseudonarcissus is higher than that among four ecotypes of Chinese Narcissus (Narcissus tazetta var. chinensis). There are seven morphological characteristics in N. pseudonarcissus presenting obvious variations with coefficients from 33.33% to 91.67%. Only five morphological characteristics in Chinese Narcissus present certain variations with coefficients from 37.04% to 51.79%. On DNA level, two clusters are distantly related too. Based on the random amplified polymorphic DNA (RAPD) markers, 13 out of 40 random primers yielded scorable polymorphisms between samples. Wide variations in banding profiles between cultivars or between ecotypes were observed with nearly every primer tested. Among 95 band positions that were scored for all the 9 narcissi, 81 are polymorphic (85.26%). Cluster analysis of the calculated similarity matrix revealed that the genetic diversity between these individuals within the same section is low. However, the genetic diversity between two sections is obviously higher. Taken together, the methods combined morphological characteristics and RAPD technique allow a deep evaluation of the variation of Narcissus on both section level and cultivar/ecotype level.

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Recent Updates on Molecular Biotechnological Intervention in Isabgol

Jaglan

Yadav

Siwach

et al. 2017

Plant Biotechnology: Recent Advancements and Developments

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Genetic diversity analysis in Plantago ovata and some of its wild allies using RAPD markers

Cited by 5 publications

References 18 publications

Expanded inverted repeat region with large scale inversion in the first complete plastid genome sequence of Plantago ovata

Expanded inverted repeat region with large scale inversion in the first complete plastid genome sequence of Plantago ovata

Genetic Diversity Analysis of Nine Narcissus Based on Morphological Characteristics and Random Amplified Polymorphic DNA Markers

Recent Updates on Molecular Biotechnological Intervention in Isabgol

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