Genetic diversity among wild and cultivated populations of Hevea brasiliensis assessed by nuclear RFLP analysis

Abstract

Restriction fragment length polymorphism was assessed in wild and cultivated populations of Hevea brasiliensis using random probes from an Hevea nuclear library. One-hundred-and-sixty-four individuals were surveyed, and the results discussed in the light of previous work performed on isozyme variation. Both studies show that germplasm collections have led to an effective enrichment of the genetic resources available for Hevea breeding, and that cultivated clones have conserved a relatively high level of polymorphism, despite their narrow genetic base and their high level of inbreeding. An equivalent level of polymorphism is revealed by random nuclear probes and isozymes. However, the genetic structuring of the diversity appears more striking using RFLP markers. Wild accessions can be divided into three genetic groups according to their geographical origin. The present results are an essential guide to the incorporation of wild material in breeding schemes.

Genetic diversity and relationship in American and African oil palm as revealed by RFLP and AFLP molecular markers

“…Therefore, markers provide an efficient method for genetic resources characterization, through which genetic diversity and organization at different levels can be assessed (Karp & Edwards, 1997;Lanaud & Lebot, 1997). RFLP has been broadly and successfully used to characterize genetic resources including some tropical perennial crops (Besse et al, 1994;N'goran et al, 1994;Jack et al, 1995;Lebrun et al, 1995;Luo & Boutry, 1995;Lerceteau et al, 1997). However, this technique requires a relatively large quantity of good quality DNA and the best results are obtained using radioisotopes, which makes RFLP technically and ecologically sensitive.…”

Analysis of Genetic Diversity and SSR Allelic Variation in Rubber Tree (Hevea brasilensis)

“…Therefore, the reasonable utilization of wild germplasm resources may be an effective way to breeding improvement and widen genetic basis (Benong, 2002;Aidi-Daslin, 2002;Clement-Demange, 2002;Varghese, 2002). In our study, the genetic diversity of wild accessions was higher than the cultivars, which consisted with Besse et al (1993Besse et al ( , 1994. Similarity coefficient between cultivars ranged from 0.51 to 0.95, with an average of 0.73, and the variations of the descendants from those crosses with high similarity coefficient would be limited.…”

Hevea Germplasm in Vietnam: Conservation, Characterization, Evaluation and Utilization

“…The largest average genetic distance among accessions within the districts was detected in the Jiparana district of Rondonia and the smallest was found in the Assis-Brasil district of Acre, of which the average genetic distance values were 0.469 and 0.139, respectively (Table 3). According to previous studies, high genetic diversity is usual in IRRDB'81 accessions (Chevallier, 1988;Besse et al, 1994;Lekawipat et al, 2003). All the accessions had unique RAPD genotypes.…”