Genetic diversity among Turkish local grape accessions (Vitis vinifera L.) using RAPD markers

Abstract

To estimate genetic relationships among 46 local grape cultivars, RAPD analysis was performed with 25 decamer primers selected from a total of 60 primers. Genetic relationships among these cultivars were determined by calculating similarity indexes, from which a dendogram was derived. There was high genetic variation among the cultivars, with values of genetic diversity ranging from 0.553 to 0.952 using the Jaccard coefficient. UPGMA analysis of a distance matrix produced a dendogram with six clusters. The relatively high genetic similarity ratios observed for the cultivars was also reflected in the dendogram. In general, no relationship was encountered between the genetic similarity ratios of the cultivars and the results of previous ampelographic analyses.

Genetic variability and relationships between and within grape cultivated varieties and wild species based on SRAP markers

“…The 19 SRAP polymorphic markers used were highly informative. The level of polymorphism obtained (78.63%) is higher than previous RAPD, ISSR, and AFLP studies (Karata and Ağaoğlu 2008;Argade et al 2009;Ergül et al 2004). Ninety combinations of primer pairs produced high number of polymorphic bands (228) among 76 genotypes, which indicates that a high level of genetic diversity exists within the germplasm of the wild species and cultivars of Vitis.…”

“…DNA-based molecular markers have been widely used for characterization and estimation of genetic diversity and cultivar characterization. Different molecular markers including: isozymes (Walker and Boursiquot 1992;Sabir et al 2009), RFLP (Bowers and Meredith 1996), RAPD (Vidal et al 1999;Karata and Ağaoğlu 2008), AFLPs (Ergül et al 2004), ISSR (Argade et al 2009), SNP (Dong et al 2010), retrotransposons-based markers (D'Onofrio et al 2010) and especially simple sequence repeat Pelsy et al 2010;Riahi et al 2010) have been used in grape genetic analysis. In grape, nuclear microsatellites have been widely used (Sefc et al 2009;Cipriani et al 2010;Laucou et al 2011).…”