Genetic diversity among toxigenic and nontoxigenicVibrio choleraeO1 isolated from the Western Hemisphere

Abstract

SUMMARYMultilocus enzyme electrophoresis was used to examine genetic relationships among and between toxigenic and non-toxigenic isolates of Vibrio cholerae 01 obtained from patients and the environment in the US Gulf Coast and surrounding areas. A total of 23 toxigenic and 23 non-toxigenic strains were examined. All the toxigenic and 7 of the non-toxigenic strains had the same alleles at 16 enzyme loci, whereas the balance of the nontoxigenic strains had 9 distinct combinations of alleles. This study suggests that all of the toxigenic strains belong to a single clone, and that while some of the non-toxigenic isolates were related, most were of diverse origin.

Biochemical Characterization of dTDP- d -Qui4N and dTDP- d -Qui4NAc Biosynthetic Pathways in Shigella dysenteriae Type 7 and Escherichia coli O7

“…Sequence analysis reveals that the two SATs share 53% identity (67% similarity), which is much higher than the levels they share with other reported SATs (Table S1 in the supplemental material). Based on the position of the amino receptor, SATs can be divided into three subgroups acting on scylloinosose (1, 13, 14), NDP-3-keto sugars (7,23,30,35), or NDP-4-keto sugars (2,5,12,27,34,36). This is also supported by the phylogenetic analysis of SATs (Fig.…”

Evolutionary Genetic Analysis of the Emergence of Epidemic Vibrio cholerae Isolates on the Basis of Comparative Nucleotide Sequence Analysis and Multilocus Virulence Gene Profiles

“…The evolutionary genetic relationships among V. cholerae strains have been examined by multilocus enzyme electrophoresis (2,11,19,20,54,58), single locus sequence analysis (7,37,38,59), and multilocus sequence analysis of housekeeping genes (9,36,40). These analyses have given conflicting results regarding the ancestry of O1 serogroup classical and El Tor biotype strains.…”

The Zymovars of Vibrio cholerae: Multilocus Enzyme Electrophoresis of Vibrio cholerae

“…The electrophoretic mobility of enzymes has been used to detect allelic variation of the respective genes in several microorganisms including Vibrio cholerae (Salles & Momen 1991, Chen et al 1991, Wachsmuth et al 1993, Beltran et al 1999.…”

“…Our previous estimated MGD was 0.326 using nearly half the strains of the present study. Chen et al (1991) 8 7 76 45 1 PD 0 0 2 0 9 25 62 35 5 P1 1 0 8 0 78 48 3 0 0 P2 2 0 119 0 15 2 0 0 0 LAP 7 0 27 0 98 5 0 1 0 ACO (aconitase hydratase); ADH (alanine dehydrogenase); IDH (isocitrate dehydrogenase); ME (malic enzyme); NSE (carboxilesterase); PGD (6-phosphogluconate dehydrogenase); MDH (malate dehydrogenase); PGM (phosphoglucomutase); GPI (glucose phosphate isomerase); G6P (glucose-6-phosphatedehydrogenase) ; PD (proline dipeptidase); P1 (peptidase leucyl-leucyl-leucine); P2 (peptidase leucyl glycil glycine) with the addition of the locus LAP: leucyl leucyl aminopeptidase (Pasteur et al 1990). …”