Genetic diversity among pearl millet maintainers using microsatellite markers

Abstract

Kapila, R. K., Yadav, R. S., Plaha, K. N., Rai, K. N., Yadav, O. P., Hash, C. T., Howarth, C. J. (2008). Genetic diversity among pearl millet maintainers using microsatellite markers. Plant Breeding, 127, (1), pp. 33-37 Sponsorship: DFID, BBSRC IMPF: 01.28 RONO: 1340 4212;1320 3012;1700 6445; 03130Genetic diversity among 70 maintainers and two pollinators of sub-Saharan and Indian origin was studied for simple sequence repeat (SSR) loci using 34 primer pairs. A total of 213 alleles were detected with an average of 6.26 alleles per locus. Polymorphic information content (PIC) ranged from 0.05 to 0.96 with a mean of 0.58 for the SSR loci. Mean PIC across the linkage groups and number of alleles in dinucleotide motifs varied significantly. The lowest PIC (0.239) for linkage group 6 indicated comparatively conserved nature of this linkage group. Genetic similarity estimates ranged from 0.05 to 0.73 with an average value of 0.29. This indicated sufficient diversity among the maintainer and pollinator lines. The 72 lines fell in five clusters, and the clustering pattern corroborated with their pedigree and characteristic traits. Pollinator ICMR 356 was more diverse from the maintainer lines analysed, and can be a potential parent for pearl millet hybrid development.Peer reviewe

EST-SSR Based Genetic Diversity and Population Structure among Korean Landraces of Foxtail Millet (Setaria italica L.)

“…This variation could have happened due to regional barriers, heterosis, and/or man made selection (Upadhyaya et al, 2008). Present research also exhibited a higher PIC (0.518) than that reported earlier in foxtail millet (0.381) and rice (0.42) (Jin et al, 2010;Lin et al, 2012), but lower than that reported in maize (0.72), pearl millet (0.58), persimmon (0.67) and perilla (0.59) (Pejic et al, 1998;Kapila et al, 2007;Seo et al, 2013;Song et al, 2012). Here, we have used a combination of di-and trinucleotides that might be the cause of variation, because a higher number of dinucleotide repeats instead of trinucleotides or higher numbers increase the genetic variability (Yang et al, 2010).…”

Development of a molecular linkage map of pearl millet integrating DArT and SSR markers

“…Such genetic differences in pearl millet have been studied previously by morphological and isozyme analysis (Tostain et al 1987;Tostain and Marchais, 1989;Tostain, 1992). Subsequently, RAPDs (Chowdari et al 1998), RFLPs (Bhattacharjee et al 2002), and SSRs (Budak et al 2003;Kapila et al 2008) and combinations of SSRs with other PCR-compatible markers (Thudi et al 2010) have been used to estimate pearl millet genetic diversity. The power of DArT fingerprinting method lies in its ability to compare different genomes at a large number of loci in a single assay, at an average cost per marker locus that is very low compared with PCR-compatible markers.…”

Molecular characterization of pearl millet [Pennisetum glaucum (L.) R. Br] inbreds using microsatellite markers

“…In previous studies, Stich et al (2010) observed more number of alleles per locus, while analyzing diversity in germplasm lines. Similarly, Kapila et al (2008) found an average of 6.26 alleles per locus, in a set of maintainer lines. In this study, lower number of alleles per locus observed, could be explained that we have used agarose gel, which has lower resolution power in comparison to polyacrylamide gel used in these studies.…”