Genetic diversity among nine species of Lippia (Verbenaceae) based on RAPD Markers

Viccini, Lyderson Facio; Costa, Deliane Cristina; Machado, M. A.; Campos, Ana Lúcia

doi:10.1007/s00606-003-0079-x

Cited by 29 publications

(20 citation statements)

References 12 publications

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“…The amount of genetic variability can also be related to the survival time of one population, since high genetic diversity normally increases the chance of adaptation and consequently influences how long it can survive (Neel and Cummings, 2003). Although it is possible to observe small populations with restricted distribution and high variability (Viccini et al, 2004;Hmeljevski et al, 2011), in general, rare and endemic species show a lower level of genetic diversity compared to widely distributed ones (Gitzendanner and Soltis, 2000).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Vriesea cacuminis (Bromeliaceae): an endangered and endemic Brazilian species

Ribeiro

Pinheiro²,

Domingues³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Data about the genetic structure can help to understand the evolutionary process of natural populations as well as to drive strategies of conservation. Vriesea cacuminis, an endemic Brazilian Bromeliad, has been found in 2 areas of Minas Gerais State. One is a legal preservation unit (Ibitipoca State Park) and the other an unprotected area (Serra Negra). The 2 areas belong to the Mantiqueira Mountain Range Complex; both are characterized by steep relief with high altitudes and by heterogenic vegetation formed by a mosaic of rocky fields and forest fragments. According to International Union for Conservation of Nature criteria, V. cacuminis is designated as "vulnerable". We examined the genetic variability and population structure of 70 individuals (3 populations) of V. cacuminis, using 16 ISSR markers. Although V. cacuminis is considered a rare species, the estimated genetic diversity was found to be relatively high (Shannon index = 0.33; percentage : 1934-1943 (2013) Genetic variability of Vriesea cacuminis of polymorphic bands = 87%). The populations were found not to be structured (AMOVA test, Φ ST = 0.16), probably due to the cross-breeding. Based on Bayesian analysis, this species includes one cluster containing the populations from Ibitipoca State Park and another cluster including the population from Serra Negra. This information will help determine strategies to maintain the genetic variability of these populations.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity of Vriesea cacuminis (Bromeliaceae): an endangered and endemic Brazilian species

Ribeiro

Pinheiro²,

Domingues³

et al. 2013

Genet. Mol. Res.

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“…The most common uses of the technique for scientific and commercial purposes include the discrimination among species for post-harvest purposes (HOSOKAWA et al, 2000), identification of crop cultivars to settle lawsuits (CONGIU et al, 2000), marker assisted selection establishment of phylogenetic position of natural populations (SGORBATI et al, 2004;TSUJI & OHNISHI, 2001;VICCINI et al, 2004), certification of lack of polymorphism between in vitro and conventionally propagated plant stands (HAO et al, 2004;LAIA et al, 2000;SALVI et al, 2002), and determination of genetic variation in wild and cultivated populations (BIRMETA et al, 2004;GUSTAFSON et al, 1999;SONNANTE et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of elephantgrass accessions through RAPD markers

Passos¹,

Machado

Vidigal

et al. 2005

Ciênc. agrotec.

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Elephantgrass pastures are limited by yield variations and reductions in forage quality and availability, thus making the search for genotypes with reduced seasonality a major concern. In order to verify the extent of genetic variability among contrasting cultivars, ten elephantgrass accessions were analyzed through DNA amplification by RAPD technique. A total of 160 DNA bands were generated with the use of 44 random primers and 23% of these bands were monomorphic for all accessions. Gel-obtained binary data (1 for presence and 0 for absence) were used for generating a genetic distance matrix, which was utilized in a UPGMA grouping analysis. Elephantgrass cultivars Cameroon and Vruckwona were the accessions mostly divergent from the others, with an average genetic distance of 0.34. The accessions with the lowest average genetic distances from the others were Pioneiro and CNPGL 27-5, both with a distance of 0.25. Overall, genetic distances ranged from 0.06 to 0.43, indicating little genetic variability for the set of accessions, despite the contrasting morphology of the studied genotypes.Index terms: molecular markers, elephantgrass, RAPD, DNA. RESUMOO cultivo do capim-elefante é limitado por variações na produção de biomassa e por reduções na qualidade e disponibilidade de forragem, tornando prioritária a busca de genótipos com menor sazonalidade de produção. Para verificar a extensão da variabilidade genética entre cultivares contrastantes, 10 acessos foram analisados via amplificação do DNA pela técnica RAPD. Foram utilizados 44 primers aleatórios, que produziram um total de 160 bandas de DNA, sendo que 23% das bandas analisadas foram monomórficas para todos os acessos. Os dados binários, originados dos géis (1-presença, 0-ausência), foram utilizados para gerar uma matriz de distâncias genéticas, que foi utilizada para uma análise de agrupamento segundo o método da média das distâncias (UPGMA). Os acessos mais divergentes dos demais foram Cameroon e Vruckwona, com distâncias genéticas médias de 0,34. Os acessos com menores distâncias genéticas médias dos demais foram Pioneiro e CNPGL 27-5, ambos com uma distância genética média de 0,25. As distâncias genéticas, considerando todas as análises, variaram de 0,06 a 0,43, indicando uma variabilidade genética pouco acentuada, embora os acessos estudados sejam bastante contrastantes em relação à morfologia e fisiologia. Termos para indexação:Marcadores moleculares, Capim-Elefante, RAPD, DNA.

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“…A set of 18 RAPD markers provided 490 fragments and permitted the analysis of interand intra-species variability in nine species of the genus Lippia (Viccini et al, 2004). Those authors highlighted L. sidoides because it shows the highest intra-species variability among the studied species.…”

Section: Discussionmentioning

confidence: 99%

“…High genetic variability was observed with 75% dissimilarity between the LGRA108 and LGRA110 accessions (Pinto et al, 2011). Species of the genus Lippia typically show high genetic variability, but the mechanisms that enable species of this genus to maintain high intra-species variability are not clear, although the fact that some species avoid endogamy is interesting (Viccini et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of Lippia sidoides Cham. and L. gracilis Schauer germplasm

Santos¹,

Pinheiro²,

Zucchi³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The conservation of plants in germplasm banks ensures the characterization and availability of these resources for future generations. The present study used DNA markers to obtain genetic information about germplasm collections of Lippia sidoides and L. gracilis, which are maintained in an Active Germplasm Bank (AGB). Genetic variability of samples in the AGB was assessed using 12 combinations of amplified fragment length polymorphism (AFLP) primers (EcoRI/MseI). Twenty simple sequence repeat primers designed for L. alba were tested to determine their transferability in L. sidoides and L. gracilis. The AFLP markers generated 789 markers. The assessed loci exhibited a moderate Shannon diversity index (I = 0.42) in both species, suggesting that the conserved accessions possess an intermediate level of genetic diversity. Twelve microsatellite loci amplified satisfactorily, and nine loci were polymorphic in each species. A total of 23, 22, and 36 alleles, with an average of 2.5, 2.4, and 3.27 alleles per locus were identified for L. sidoides and L. gracilis accessions in the AGB, and Lippia sp sampled plants, respectively. Analyses of genetic structure permitted the identification of three different groups using both sets of markers, of which two were representative of L. sidoides. The information generated in this study may help to create, expand, and maintain collections of these species and may assist in genetic-breeding programs.

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Genetic diversity among nine species of Lippia (Verbenaceae) based on RAPD Markers

Cited by 29 publications

References 12 publications

Genetic diversity of Vriesea cacuminis (Bromeliaceae): an endangered and endemic Brazilian species

Genetic diversity of Vriesea cacuminis (Bromeliaceae): an endangered and endemic Brazilian species

Molecular characterization of elephantgrass accessions through RAPD markers

Genetic diversity of Lippia sidoides Cham. and L. gracilis Schauer germplasm

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