Genetic diversity among maize varieties revealed by phenotypic descriptors and RAPD profiles

Handi, Suresh; Sasidharan, N.; Chakraborty, Sudeshna; Macwana, Sneha; Trivedi, Ruchi; Punwar, Bhupendra Singh; Vala, Ashish G

doi:10.4038/jas.v8i2.5744

Cited by 3 publications

(2 citation statements)

References 9 publications

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“…This is absolutely different from that obtained in rice (Oryza sativa) showing a 100% polymorphism when amplified using OPK-16 (Rajani et al, 2013). Slightly lower polymorphism (93.75%) with OPA-16 in maize (Zea mays) was found (Handi et al, 2013).…”

Section: Resultsmentioning

confidence: 95%

RAPD Profiles of Rhynchostylis gigantea (Lindl.) Ridl. Collected from Puspa Nirmala Orchids Banyumas, Central Java

Susanto

Romadhoni

Dwiati

2022

molekul

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Rhynchostylis gigantea (Lindl.) Ridl. is an orchid species spread over Southeast Asia countries. This species is very popular among ornamental plant collectors, especially due to its densely pack inflorescences. Hence, it is commercially found in many ornamental plant nursuries, such as Puspa Nirmala Orchids Banyumas, Central Java. Further development of the species should be supported by scientific data, particularly regarding the genetic variation. One of the molecular markers commonly used to study genetic variation is Random Amplified Polymorphic DNA (RAPD). This study aims to assess genetic variation of R. gigantea cultivars of Puspa Nirmala Orchids Banyumas collection by RAPD profiles. Genomic DNAs were extracted from leaf samples of eight R. gigantea individuals, while RAPD markers were amplified using five random primers (OPA-15, OPK-16, OPP-15, OPP-08 and OPO-08). Descriptive analysis was employed on the data obtained. It was revealed that all of the primers resulted in a 100% monomorphism. This indicates an extremely low genetic variation among R. gigantea population of Puspa Nirmala Orchids collection, which is probably due to the same origin from a selected hybrid of the same crosses.

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Section: Resultsmentioning

confidence: 95%

RAPD Profiles of Rhynchostylis gigantea (Lindl.) Ridl. Collected from Puspa Nirmala Orchids Banyumas, Central Java

Susanto

Romadhoni

Dwiati

2022

molekul

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“…The total number of bands for each primer was different, from 7 bands (OPC-07) to 11 (OPB-12 and OPA-15), while the average polymorphic percentage was 94% (Figure 3). The results of the study by Handi et al (2013) showed that the test results of 56 maize genotypes using 11 primers produced an average percentage of 97% polymorphic bands. Furthermore, the results of Berhitu et al (2019) showed that the results of testing Southwest Maluku local maize-Indonesia (var.…”

Section: Molecular Characterizationmentioning

confidence: 99%

Morphological and molecular characterization of maize lines tolerance to drought stress

Amzeri

Badami

SANTOSO³

et al. 2022

Biodiversitas

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Abstract. Amzeri A, Badami K, Santoso SB, Sukma KPW. 2022. Morphological and molecular characterization of maize lines tolerance to drought stress. Biodiversitas 23: 5844-5853. The early step in assembling varieties with early maturity, high productivity, and resistance to drought stress is to carry out morphological and molecular characterization among lines resistant to drought stress. The research aimed to characterize the morphology and molecular characteristics of maize lines resistant to drought stress to develop breeding and cultivation programs. The research used a randomized complete block design with twenty genotypes (maize lines) as treatment and three replicates. A total of 57 morphological characters and seven RAPD primers were used to assess the relationship based on morphological characters and RAPD markers. The results showed that the similarity coefficient based on the morphological characters was 0.69-0.97, while the similarity coefficient based on the RAPD marker was 0.64-0.95. The phenotypic diversity coefficient (PDC) was greater than the Genotypic Diversity Coefficient (GDC) in all observed quantitative characters. The broad sense heritability values of the tested maize lines ranged from 0.83 to 0.99. Production per hectare was significantly positively correlated with eight tested quantitative characters. G1 and G15 were the genotypes that can be used as parents to assemble hybrid maize varieties, while G10, G11, G16, and G17 could be used as maize for cultivation programs.

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Chauhan¹,

Jasrai²,

Pandya³

et al. 2015

JBPR

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The aim of this study was to evaluate the genetic diversity of Indian improved six major cereal crops like rice, wheat, maize, barley, pearl millet and sorghum varieties. The diversity of thirty-six varieties of six major cereal crops (rice, wheat, maize, barley, pearl millet and sorghum) was estimated by Random Amplified Polymorphic DNA (RAPD) technique using 20 primers. RAPD analysis detected a total of 168 DNA fragments of which 107 (63.69%) were polymorphic. The genetic similarity matrix (based on Dice index) ranged from 0.435 to 0.81. These indices were used to construct a dendrogram using average linkage between groups. Results illustrate the potential of RAPD markers showed considerable potential for estimating genetic diversity among our six major cereal crops cultivars. The study provides valuable information for cultivars fingerprinting at a molecular level and can be used to assist efficient selection in six major cereal crops breeding strategies aiming at achieving sustainability in six major cereal crops production in India.

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Genetic diversity among maize varieties revealed by phenotypic descriptors and RAPD profiles

Abstract: Fifty

Cited by 3 publications

References 9 publications

RAPD Profiles of Rhynchostylis gigantea (Lindl.) Ridl. Collected from Puspa Nirmala Orchids Banyumas, Central Java

RAPD Profiles of Rhynchostylis gigantea (Lindl.) Ridl. Collected from Puspa Nirmala Orchids Banyumas, Central Java

Morphological and molecular characterization of maize lines tolerance to drought stress

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