Genetic diversity among Fusarium species associated with sorghum stalk rot in Southern Ethiopia

Chala, Alemayehu

doi:10.5897/ajb2018.16725

Cited by 3 publications

(1 citation statement)

References 39 publications

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“…In sorghum, Fusarium species are known as phytopathogens and producers of mycotoxins [4,17,51]. Fusarium species that cause root rot, stalk rot, ear rots and those which cause grain mold are of major concern, as they significantly reduce yield in fields and contaminate stored grain through the production of mycotoxins, which could pose health risks to humans and animals [52,53]. The diversity and identity of Fusarium species infecting sorghum is still limited, especially in Africa [52].…”

Section: Discussionmentioning

confidence: 99%

Baseline Data of the Fungal Phytobiome of Three Sorghum (Sorghum bicolor) Cultivars in South Africa using Targeted Environmental Sequencing

Pambuka

Kinge

Ghosh

et al. 2021

JoF

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Plant-associated fungi, or the mycobiome, inhabit plant surfaces above ground, reside in plant tissues as endophytes, or are rhizosphere in the narrow zone of soil surrounding plant roots. Studies have characterized mycobiomes of various plant species, but little is known about the sorghum mycobiome, especially in Africa, despite sorghum being one of the most important indigenous and commercial cereals in Africa. In this study, the mycobiome associated with above- and below-ground tissues of three commercial sorghum cultivars, as well as from rhizosphere and surrounding bulk soil samples, were sequenced using targeted sequencing with the Illumina MiSeq platform. Relative abundance differences between fungal communities were found between above-ground and below-ground niches, with most differences mostly in the dominant MOTUs, such as Davidiellaceae sp. (Cladosporium), Didymellaceae sp. 1 (Phoma), Fusarium, Cryptococcus and Mucor. Above-ground communities also appeared to be more diverse than below-ground communities, and plants harboured the most diversity. A considerable number of MOTUs were shared between the cultivars although, especially for NS5511, their abundances often differed. Several of the detected fungal groups include species that are plant pathogens of sorghum, such as Fusarium, and, at low levels, Alternaria and the Ustilaginomycetes. Findings from this study illustrate the usefulness of targeted sequencing of the ITS rDNA gene region (ITS2) to survey and monitor sorghum fungal communities and those from associated soils. This knowledge may provide tools for disease management and crop production and improvement.

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Section: Discussionmentioning

confidence: 99%

Baseline Data of the Fungal Phytobiome of Three Sorghum (Sorghum bicolor) Cultivars in South Africa using Targeted Environmental Sequencing

Pambuka

Kinge

Ghosh

et al. 2021

JoF

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Diversity and pathogenicity of Fusarium spp. isolated from cultivated sorghum stems and roots in eastern Australia

Gunasinghe,

Vaghefi,

Shivas

et al. 2024

Plant Pathology

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Stalk and root rots of cultivated sorghum (Sorghum bicolor) are caused by several Fusarium species worldwide. This study evaluated Fusarium diversity, pathogenicity and population structure amongst 212 isolates obtained from 169 sorghum plants from commercial field crops in eastern Australia. Sequences of translation elongation factor‐1α (tef‐1α), RNA polymerase II largest subunit (rpb1), RNA polymerase II second largest subunit (rpb2) and calmodulin (cmdA) were used to construct multilocus phylogenies that enabled the identification of 16 Fusarium species in Fusarium chlamydosporum species complex (FCSC), Fusarium fujikuroi species complex (FFSC), Fusarium incarnatum‐equiseti species complex and Fusarium oxysporum species complex (FOSC). The majority of isolates (n = 171) belonged to FFSC. The pathogenicity of 17 selected isolates was determined by artificial inoculation of sorghum seedlings and completing Koch's postulates. Isolates of species in FFSC were significantly (p < 0.05) more aggressive as root pathogens in sorghum seedlings than isolates of other species tested and widely distributed across all sampling sites. Amongst the 35 isolates of FOSC, 26 belonged to Fusarium cili, which is only known as an endophyte from healthy roots of Rosa roxburghii in China. Fusarium sporodochiale (in FCSC) and Fusarium contaminatum (in FOSC) are reported as sorghum seedling root rot pathogens for the first time.

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Phylogenetically Diverse Fusarium Species Associated with Sorghum (Sorghum Bicolor L. Moench) and Finger Millet (Eleusine Coracana L. Garten) Grains from Ethiopia

Chala

Degefu²,

Brurberg

2019

Diversity

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Fusarium is one of the most diverse fungal genera affecting several crops around the world. This study describes the phylogeny of Fusarium species associated with grains of sorghum and finger millet from different parts of Ethiopia. Forty-two sorghum and 34 finger millet grain samples were mycologically analysed. All of the sorghum and more than 40% of the finger millet grain samples were contaminated by the Fusarium species. The Fusarium load was higher in sorghum grains than that in finger millet grains. In addition, 67 test isolates were phylogenetically analysed using EF-1α and β-tubulin gene primers. Results revealed the presence of eight phylogenetic placements within the genus Fusarium, where 22 of the isolates showed a close phylogenetic relation to the F. incarnatum–equiseti species complex. Nevertheless, they possess a distinct shape of apical cells of macroconidia, justifying the presence of new species within the Fusarium genus. The new species was the most dominant, represented by 33% of the test isolates. The current work can be seen as an important addition to the knowledge of the biodiversity of fungal species that exists within the Fusarium genus. It also reports a previously unknown Fusarium species that needs to be investigated further for toxin production potential.

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Genetic diversity among Fusarium species associated with sorghum stalk rot in Southern Ethiopia

Cited by 3 publications

References 39 publications

Baseline Data of the Fungal Phytobiome of Three Sorghum (Sorghum bicolor) Cultivars in South Africa using Targeted Environmental Sequencing

Baseline Data of the Fungal Phytobiome of Three Sorghum (Sorghum bicolor) Cultivars in South Africa using Targeted Environmental Sequencing

Diversity and pathogenicity of Fusarium spp. isolated from cultivated sorghum stems and roots in eastern Australia

Phylogenetically Diverse Fusarium Species Associated with Sorghum (Sorghum Bicolor L. Moench) and Finger Millet (Eleusine Coracana L. Garten) Grains from Ethiopia

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