Genetic diversity among fourteen different Fusarium species using RAPD marker

Abstract

We report genetic diversity of total fourteen different Fusarium species by RAPD-PCR analysis using 25 random primers. The genus Fusarium is food borne pathogen responsible for T-2 toxin production which affects human and animal health. In the present study, total 14-different species of Fusarium were analyzed on the basis of genetic diversity using RAPD method. A dendrogram was developed by UPGMA method. RAPD analysis was carried out by using 25 different universal primers each of them consisted of 10 bases. Genetic similarity coefficients between pair wise varied from 0.00 to 0.9 based on an unweighted paired group method of arithmetic average (UPGMA) cluster analysis. RAPD-PCR technique can be used as an important tool for the genetic differentiation Fusarium species.

Short Communication: Genetic diversity among Fusarium isolates from cereals in Iran assessed using RAPD marker

“…Thirteen different Fusarium isolates were subjected to RAPD-PCR (Table 1). Extracted DNA was amplified by 4 selective primers (Bonde et al 2013) (Table2). PCR conditions were: an initial denaturation step at 94°C for 2 min, 35 cycles of 94°C for 30 s, 40°C for 60 s, 72°C for 2 min and a final extension at 72°C for 5 min.…”

“…Genetic polymorphism between isolates of F. solani was studied by Gupta et al (2009) in India by RAPD. Smith et al (2001), Arif et al (2011), Niessen (2007), Gupta et al (2009), and Bonde et al (2013) reported genetic variation among Fusarium isolates by RAPD marker.…”

“…Fusarium isolates in RAPD analyses Primers for RAPD analysis(Bonde et al 2013) Identified Fusarium species by morphological characters…”

Molecular detection and genetic characterisation of fumonisin producingfusariumisolates from rice cultivars

“…Fusarium mycotoxin such as fumonisin has adverse health effects on animals and human hence RAPD-PCR based genetic polymorphism assay was carried out among the 28 Fusarium isolates which were isolated from popular rice varieties of India. Bonde et al (2013) obtained 180 polymorphic RAPD loci (amplified fragments were ranged 1 kb to 3.0 kb) in 14 mycotoxic Fusarium species with 25 primers and suggested RAPD marker to be a powerful tool to analyze the genetic variation among the Fusarium species. Similarly Sharma et al (2006) and Belabid et al (2004) amplified 134 and 8 polymorphic markers with 10 and 6 primers respectively.…”

“…Many workers have extensively studied PCR Based genetic variability or polymorphism. The Randomly Amplified Polymorphic DNA (RAPD) assay in which DNA segments are amplified by arbitrary primers, has been used for genetic characterisation of Fusarium species and other mycotoxigenic fungi (Nelson et al, 1997;Mostafa et al, 2002;Belabid et al, 2004;Sharma et al, 2006;Lourenço et al, 2007;Gupta et al, 2009;Mishra et al, 2010;Mohammadian et al, 2011;Gupta, 2012;Bahmani et al, 2012;Bonde et al, 2013;Ebadi et al, 2013). RAPD-genetic characterisation of Fusarium species from animal feed have already been carried out based on fumonisin production.…”