Genetic Diversity Among Durian (Durio Zibethinus Murr.) Populations From Nias Island, Indonesia Using Rapd Markers

Hannum, Saleha; Wahyuningsih, Hesti; Sinaga, Riyanto; Pasaribu, Nursahara; Hartanto, Adrian

doi:10.15666/aeer/1805_73377351

Cited by 6 publications

(5 citation statements)

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“…The percentage of fragment polymorphic in this study was higher than previously reported by Sheet et al (2018) in mulberry cultivars (59.62%), Kumar et al (2019) in papaya (58.36%), and El-Khayat & Aseel (2020) in four Egyptian Mandarin cultivars (53.81%). The level of polymorphism obtained in the present study is lower than in those previous studies reported by Sundari et al (2017) in durian (86.50%), Hannum et al (2020) in durian (100%), Fadillah et al (2022) in Rukam (93.42%). However, the degree of polymorphism obtained in the present study is almost similar to that obtained in an earlier study using the RAPD marker on the citrus cultivar (80.63%) (Shahzadi et al 2016), Prunus salisina L (81.60%) (Li et al 2022).…”

Section: Genetic Variationcontrasting

confidence: 82%

Molecular identification of mother trees of four matoa cultivars (Pometia pinnata Forst & Forst) from Pekanbaru City, Indonesia using RAPD markers

et al. 2023

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Abstract. Zulfahmi, Pertiwi SA, Rosmaina, Elfianis R, Gulnar Z, Zhaxybay T, Bekzat M, Zhaparkulova G. 2023. Molecular identification of mother trees of four matoa cultivars (Pometia pinnata Forst & Forst) from Pekanbaru City, Indonesia using RAPD markers. Biodiversitas 24: 1524-1530. Pekanbaru city has four matoa (Pometia pinnata Forst & Forst) cultivars, i.e. red matoa, yellow matoa, green matoa, and black matoa, but the knowledge of the genetic variation among matoa cultivars is not available. The objective of this study was to assess the genetic variation among matoa cultivars using Random amplified polymorphism DNA (RAPD) markers and to identify the specific marker to distinguish among matoa cultivars. Eighteen primers were initially screened and four matoa cultivars were analyzed with twelve primers using the RAPD marker. The results of this study found that the twelve selected RAPD primers generated 39 fragments, with fragment sizes ranging from 200 to 1500 bp. The percentage of fragment polymorphic was 80.41%, indicating the high genetic variation of the matoa cultivar. The high genetic variation of matoa in this study is caused by cross-pollinated among matoa cultivars in the field. Eleven primers can differentiate four matoa cultivars with specific bands. The obtaining unique bands can be utilized by breeders and farmers as a basis to select the parents for the genetic improvement of Matoa, cultivars or clones protection, detection of seedling purity in a nursery, and to verify the originality of the seedlings that will be cultivated.

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Section: Genetic Variationcontrasting

confidence: 82%

Molecular identification of mother trees of four matoa cultivars (Pometia pinnata Forst & Forst) from Pekanbaru City, Indonesia using RAPD markers

et al. 2023

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“…By leveraging this scientific breakthrough, the present study assessed the genetic base of durian plantations in Hainan, aiming to develop molecular markers for durian breeding efforts. Genetic research on durian has developed relatively slowly due to inadequate genetic information and effective molecular marker systems, with those reported so far based on morphological markers [17][18][19][20]. The present study used leaf samples of durian in the plantation sites in Hainan whose genetic base largely remain unknown, coupled with the same genotype names but differences in morphology, and vice versa (Table 1), to elucidate the genetic bases to ensure effective conservation and utilization in breeding programs.…”

Section: Discussionmentioning

confidence: 99%

“…In the past, attempts were made to characterize durian germplasm and its relatives via morphological markers. However, this is inefficient due to multigenic interaction and is heavily affected by environmental factors [17][18][19][20]. Mursyidin, et al [21] characterized 18 exotic Durio spp.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Diversity and Discovery of Molecular Markers in Durian (Durio zibethinus L.) in China

et al. 2022

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Durian (Durio zibethinus L.) is a crop of economic and health importance globally. Efforts are being made to revamp China’s only successful commercial-scale durian plantations in Hainan; however, their genetic base is unknown. Therefore, the present study was undertaken to assess the genetic base and population structure of 32 genotypes in durian plantation sites in Hainan, China, and develop simple sequence repeat (SSR) markers by whole genome sequencing through restriction site-associated DNA sequencing technology to facilitate germplasm conservation and breeding. The results from identity by state (IBS), phylogenetic tree, population structure, and principal component analysis grouped the 32 genotypes into two clusters/sub-populations. Based on IBS, genotypes in Cluster I are largely duplicated genotypes; however, results from the model-based population structure demonstrated that most of the genotypes in Sub-population II shared a common genetic background with those in Sub-population I/Cluster I. The results revealed that the core durian collection in the plantation sites in Hainan include D24, D101, MSW, JH, D163, HFH, and NLX-5. In addition, we developed a total of 79,178 SSR markers with varied lengths and amplicon sizes. The genetic diversity and population structure reported in this study will be useful for durian conservation and utilization. In addition, the discovered and developed SSR markers will lay the foundation for molecular breeding via marker-assisted selection, quantitative trait loci mapping, and candidate gene discovery and validation.

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“…Amplification was carried out in a SensoQuest thermocycler with a cycle programmed for 45 cycles of each of the following conditions: initial denaturation at a temperature of 94C for 2 minutes, followed by denaturation at 94C for 1 minute, annealing at 34C for 1 minute, elongation at 72C for 2 minutes 20 seconds, and the final condition was elongation at 72C for 10 minutes. OPA-02 TGCCGAGCTG [24] OPA-03 AGTCAGCCAC [25] OPA-07 GAAACGGGTG [25] OPA-08 GTGACGTAGG [26] OPA-10 GTGATCGCAG [25] OPA-13 CAGCACCCAC [25] OPA-16 AGCCAGCGAA [27] OPB-08 GTCCACACGG [28] OPB-10 CTGCTGGGAC [29] Electrophoresis…”

Section: Rapd Primer Selection and Dna Amplificationmentioning

confidence: 99%

Genetic Variation of Ceiba pentandra (L.) Gaertn. from Three Populations in West Sumatra, Indonesia Based on RAPD Markers

Hefzi,

Mansyurdin,

Maideliza

et al. 2023

IJPSAT

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Ceiba pentandra (L.) Gaertn. is one of the cotton-producing plants. However, there is a population decline in some areas, resulting in a decrease in genetic variation. This study aims to determine intrapopulation and interpopulation genetic variation and genetic differentiation of C. pentandra in three populations in West Sumatra. The research was conducted with the descriptive method using molecular data and sample collection with the survey method. Analysis of genetic variation using RAPD (Random Amplified Polymorphic DNA) markers on 15 individual plants collected from three populations, namely Solok, Pesisir Selatan, and Padang Pariaman. The results showed that primers OPA-01, OPA-02, and OPB-10 could detect polymorphism. The value of intrapopulation genetic variation was highest in the South Coastal population (H = 0.1857) and lowest in the Solok population (H = 0.1228). Interpopulation genetic variation (DST = 0.0326) was lower than intrapopulation genetic variation (Hs = 0.1607), with a low genetic differentiation value (GST = 0.1686) and a high gene flow value (Nm = 2.4660). Cluster analysis showed that the Pesisir Selatan and Solok populations had the farthest genetic distances (0.0580). UPGMA analysis shows that accessions do not cluster specifically based on their population.

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Genetic Diversity Among Durian (Durio Zibethinus Murr.) Populations From Nias Island, Indonesia Using Rapd Markers

Cited by 6 publications

References 0 publications

Molecular identification of mother trees of four matoa cultivars (Pometia pinnata Forst & Forst) from Pekanbaru City, Indonesia using RAPD markers

Molecular identification of mother trees of four matoa cultivars (Pometia pinnata Forst & Forst) from Pekanbaru City, Indonesia using RAPD markers

Assessment of Genetic Diversity and Discovery of Molecular Markers in Durian (Durio zibethinus L.) in China

Genetic Variation of Ceiba pentandra (L.) Gaertn. from Three Populations in West Sumatra, Indonesia Based on RAPD Markers

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