Genetic diversity among a complex of cereal cyst nematodes inferred from RFLP analysis of the ribosomal internal transcribed spacer region

Abstract

This study examined the restriction polymorphism (RFLP) of the nuclear ribosomal DNA in Heterodera avenae, H. filipjevi, H. mani, H. latipons, and the taxonomically unclear Gotland strain in order to establish a molecular characterization and phylogenetic relationships in the complex of cereal cyst nematodes (CCN). The internal transcribed spacer (ITS) and 5.8S rDNA were amplified by PCR from a single female or a cyst of 27 different geographic isolates of the CCN complex and one population of H. schachtii, used as outgroup. The amplified product was 1.2 kb long and 14 of 15 enzymes produced restriction fragments for each isolate. Relationships between populations were determined from UPGMA analysis based on distance values calculated from RFLP data. Digestions with TaqI clearly differentiated H. avenae, H. latipons, and a group composed of H. filipjevi and the Gotland strain. Six endonucleases (HaeIII, HinfI, ItaI, PstI, TaqI, and Tru9I) produced the same restriction pattern with H. filipjevi and the Gotland strain, and both were clearly separated from H. avenae with PstI. Restriction sites have revealed a mixture of the species H. latipons and H. avenae, and possible infraspecific variation in H. avenae. The inferred phylogenetic relationships of species in the CCN complex are in agreement with their morphological characterization.

Morphological and molecular identification of cereal cyst nematodes from the eastern Mediterranean region of Turkey

“…In this study, intraspecific polymorphism based on sequence analysis was shown among populations of H. avenae, as in previous studies (Bekal et al, 1997;Subbotin et al, 1999;Handoo, 2002;Rivoal et al, 2003). The H. avenae populations in this study consisted of 2 subgroups.…”

“…The results of our study did not show any intraspecific polymorphism between H. filipjevi populations. Furthermore, Bekal et al (1997) did not detect any genetic variation among the different populations of this species. The H. latipons populations in this study consisted of 2 subgroups.…”

Age structure of populations of the cyst nematode Heterodera Filipjevi (Heteroderidae: Nematoda) on different wheat and barley varieties

“…All three economically important species were found in Bulgaria but the latest record concerns H. filipjevi (Bekal et al, 1997). In further studies the species appeared to be much more common in cereal productive areas of the country than H. avenae and H. latipons -it was foundin 21 localities (regions of Vidin, Pleven, Velico Tarnovo, Shumen, Varna, Burgas, Sliven, Haskovo, Stara Zagora, Plovdiv and Pazardjik) of all 31 where cereal cysts nematodes were found (Ilieva and Iliev, 2013).…”

Behavior of cereal’s varieties in the presence of <i>Heterodera avenae</i> woll (heteroderidae, tylenchida) cyst nematode in Tiaret Area (Algeria)

“…A propos des travaux effectués sur l'analyse de l'ADN, les techniques moléculaires ont montré une variabilité génétique au sein des populations d'Heterodera avenae [31,14] ; [32][33][34]. En Egypte [35] a étudié l'effet des amendements organiques sur la dynamique de population de H. zeae.…”