Genetic Divergence among Populations of the Yellow-Bellied Slider Turtle (Pseudemys scripta) Separated by Aquatic and Terrestrial Habitats

Scribner, Kim T.; Evans, Julianne; Morreale, Stephen J.; Smith, Michael H.; Gibbons, J. Whitfield

doi:10.2307/1444951

Cited by 36 publications

(19 citation statements)

References 17 publications

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“…Glyptemys insculpta is a long-lived species with a reported lifespan of [50 years (Oliver 1955). Limited but continuous exchange of individuals over many generations would result in genetic homogeneity as has been suggested for other freshwater turtles (Kiester et al 1982;Scribner et al 1986). Dispersal at the level suggested by Nm estimates calculated in the present study would account for the absence of significant genetic divergence among aggregations.…”

Section: Resultssupporting

confidence: 59%

Genetic diversity and population genetic structure of the Wood Turtle (Glyptemys insculpta) at Delaware Water Gap National Recreation Area, USA

2008

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Glyptemys insculpta is considered to be one of the most endangered freshwater turtles in North America. Here microsatellite markers were employed to investigate the genetic variation and population structure of G. insculpta at Delaware Water Gap National Recreation Area (USA). Seven microsatellites revealed high allelic variation with 13-30 alleles per locus. Observed and expected heterozygosities per locus ranged from 0.875-0.925 to 0.888-0.952, respectively. Pairwise estimates of population structure (h) ranged from 0.000-0.013 to h estimated over all loci and aggregations was not significantly different from zero. Gene flow (Nm) was high and ranged from 19 migrants per generation to infinity in pairwise comparisons. No significant relationship between geographic distance and genetic distance was detected. These data indicate that G. insculpta at DEWA represent a single, genetically diverse management unit for conservation.

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Section: Resultssupporting

confidence: 59%

Genetic diversity and population genetic structure of the Wood Turtle (Glyptemys insculpta) at Delaware Water Gap National Recreation Area, USA

2008

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“…Unfortunately, until relatively recently very little information existed on turtle and tortoise allozymes. Recent exceptions include Geochelone elephantopus (Marlow and Patton, 1981) and Pseudemys scripta (Scribner et al, 1984(Scribner et al, , 1986. Methods are now available that allow enzymes expressed in blood to be easily obtained, resolved, and identified.…”

Section: Landmentioning

confidence: 99%

Allozyme Variation in Mojave Populations of the Desert Tortoise, Gopherus agassizi

Rainboth¹,

Buth²,

Turner³

1989

Copeia

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“…3). Scribner et al [14] found that populations that live close to each other have higher genetic identities by the effect of gene flow. The obvious pertinence between geographic isolation and genetic distance shows that the genetic differentiations between populations are mainly because of the geographic proximity.…”

mentioning

confidence: 99%

Microsatellite Based Genetic Diversity and Differentiation of Common Carp, Cyprinus carpio in Rajasthan (India)

Singh

Sharma

Jain

et al. 2015

Natl. Acad. Sci. Lett.

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Five microsatellite loci (MFW1, MFW3, MFW4, MFW5 and MFW7) were used to study the genetic diversity and characterization of different strains (Maharashtra, Local, Koi and Amour) of common carp (Cyprinus carpio L.). The average number of alleles across all loci was highest in Koi (11.4) followed by Maharashtra (11.0) and Local strains (9.0). The amour had lowest (6.2) mean number of alleles. The mean observed heterozygosity (Ho) ranged between 0.45 and 0.62, while expected heterozygosity (He) 0.32-0.68. Polymorphic information content mean values varied from 0.58 to 0.74. The Nm and the FST values indicated a low level of gene flow and high level of differentiation. The highest genetic distance was observed between the Amour and the Local strains, while the lowest was between Maharashtra and Local strains. The FST value ranged between 0.028 and 0.207.The common carp, Cyprinus carpio is a freshwater Cyprinid originated from Eurasia. This is an exotic spp. in Indian waters and its gene pool is expected to change due to isolation and adaptation process. To monitor the genetic changes, different types of genetic markers especially microsatellite are used. In the past decade, microsatellites have become a preferred tool for breeding applications and genetic fingerprinting due to their high information content. Microsatellite markers allow for fingerprinting animals for traits reflecting genotypic differences irrespective of phenotype displayed and provide a molecular means of tracking heritable elements. This guidance is important in the selection of brood stock since it improves the accuracy and quality of the breeding prediction for future generations. The aim of the study was to obtain insight into the relationships within and among different strains of common carp. The strains of common carp (Maharashtra, Local, Koi and Amour) maintained at Aquaculture Research and Seed Unit, Directorate of Research, MPUAT, Udaipur were used for present study. The caudal fin (approximately 100 mg) samples from 80 (20 from each strain) common carp were collected. The fin samples were kept in Eppendorf micro-tubes with absolute ethanol. Genomic DNA was extracted from tissue using a protinase K, phenol: chloroform protocol [1].Polymerase chain reaction (PCR) was done in a C1000 TM Real Time PCR (Bio-Rad) and reactions carried out in 15 ll reaction volume containing 10.88 ll of mili Q water, 1.5 ll of 1 9 PCR buffer, 0.6 ll of dNTP, 0.4 ll of reverse primer (0.3 pmol), 0.4 ll of forward (M-13 labeled) primer (0.3 pmol), 0.2 ll of tailed primer (0.3 pmol) 0.2 ll Taq and 1.0 ll of template. The amplification conditions recommended by Crooijmans et al. [2] were followed with some modification especially in annealing temperature. The PCR cycles were: pre-amplification denaturation at 94°C for 3 min, then 30 s at 94°C, 30 s at the respective annealing temperature (56°C

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Genetic Divergence among Populations of the Yellow-Bellied Slider Turtle (Pseudemys scripta) Separated by Aquatic and Terrestrial Habitats

Cited by 36 publications

References 17 publications

Genetic diversity and population genetic structure of the Wood Turtle (Glyptemys insculpta) at Delaware Water Gap National Recreation Area, USA

Genetic diversity and population genetic structure of the Wood Turtle (Glyptemys insculpta) at Delaware Water Gap National Recreation Area, USA

Allozyme Variation in Mojave Populations of the Desert Tortoise, Gopherus agassizi

Microsatellite Based Genetic Diversity and Differentiation of Common Carp, Cyprinus carpio in Rajasthan (India)

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