Genetic distinctiveness of safflower cultivars of India and Mexico as revealed by SSR markers

Kadirvel, P.; Ravi, D.; Mukta, N.; Montoya-Coronado, M.C.L.; Ghuge, S.B.; Singh, Jogendra; Singh, Vrijendra; Shinde, Suhas; Deshmukh, S. N.; Yadav, Preeti; Varaprasad, K. S.

doi:10.1017/s1479262116000186

Cited by 8 publications

(4 citation statements)

References 45 publications

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“…Molecular markers are considered important tools for the identification of cultivars, for allowing polymorphism analysis directly in the DNA [ 8 , 9 ]. In this respect, microsatellite or SSR markers have being extremely useful for cultivar distinction, particularly in the case of species with a narrow genetic base, where DNA fingerprinting may be highly efficient.…”

Section: Introductionmentioning

confidence: 99%

Distinctness of Brazilian common bean cultivars with carioca and black grain by means of morphoagronomic and molecular descriptors

et al. 2017

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Common bean (Phaseolus vulgaris L.) is one of the most important legumes for human consumption and is a staple food in the diet of the population of some countries of Latin America, Africa and Asia. The distinction between cultivars is based predominantly on morphological descriptors, which proved inefficient for the differentiation of some cultivars. This study had the objective of describing, distinguishing and evaluating the agronomic potential of 39 common bean cultivars of the carioca and black grain groups registered for cultivation in Brazil, based on 49 morphoagronomic descriptors and microsatellite (simple sequence repeat -SSR) markers. The morphoagronomic traits of each commercial group were characterized in four environments. Thirty-seven SSR markers were used for the molecular description. The morphological data, analyzed by the Shanonon-Weaver index, detected low variability among cultivars for qualitative data. On the other hand, the estimates of variance analysis, relative importance of the traits and hierarchical grouping analysis applied to the quantitative variables showed that the descriptors related to plant morphology were the most important for the carioca group, and those related to seed morphology were determining for the black group. The genetic parameters estimated for SSR markers by hierarchical and Bayesian cluster analysis identified 116 alleles, with 33 and 30 polymorphic loci and 24 and 22 private alleles for the carioca and black groups, respectively. The combined use of morphoagronomic and molecular descriptors improves the distinguishability of cultivars, contributing in a more efficient way to breeding and to the protection of cultivars.

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Section: Introductionmentioning

confidence: 99%

Distinctness of Brazilian common bean cultivars with carioca and black grain by means of morphoagronomic and molecular descriptors

et al. 2017

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“…For example, Kiran et al (2017) used 44 SSR markers for genetic diversity evaluation, linkage disequilibrium and population structure analysis among 148 accessions of safflower. Moreover, assessment of genetic distinctiveness among Indian and Mexican cultivars of safflower (Kadirvel et al, 2017), and association mapping of agronomic traits (Ambreen et al, 2018) were reported.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of simple sequence repeats in cDNA sequences of safflower (Carthamus tinctorius) reveals the importance of SSR-containing genes for cell biology and dynamic response to environmental cues

Ahmadi¹,

Ahmadikhah

2022

Front. Plant Sci.

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Safflower (Carthamus tinctorius) is a diploid crop plant belonging to the family Asteraceae and is well known as one of important oilseed crops due to edible oil containing unsaturated fatty acids. In recent years it is gaining increased attention for food, pharmaceutical and industrial uses, and hence the updating its breeding methods is necessary. Genic simple sequence repeats (SSRs) in addition of being desire molecular markers, are supposed to influence gene function and the respective phenotype. This study aimed to identify SSRs in cDNA sequences and further analysis of the functional features of the SSR-containing genes to elucidate their role in biological and cellular processes. We identified 1,841 SSR regions in 1,667 cDNA sequences. Among all types of repeats, trinucleotide repeats were the most abundant (35.7%), followed by hexanucleotide (29.6%) and dinucleotide repeats (22.0%). Thirty five SSR primer pairs were validated by PCR reaction, detected a high rate of polymorphism (>57%) among safflower accessions, physically mapped on safflower genome and could clearly discriminate the cultivated accessions from wild relatives. The cDNA-derived SSR markers are suitable for evaluation of genetic diversity, linkage and association mapping studies and genome-based breeding programmes. Occurrence of SSR repeats in biologically-important classes of proteins such as kinases, transferases and transcription factors was inferred from functional analyses, which along with variability of their repeat copies, can endow the cell and whole organism the flexibility of facing with continuously changing environment, and indicate a structure-based evolution mechanism of the genome which acts as an up-to-dating tool for the cell and whole origanism, which is realized in GO terms such as involvement of most SSR-containing genes in biological, cellular and metabolic processes, especially in response to stimulus, response to stress, interaction to other organisms and defense responses.

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“…These maps have used only a small number of marker loci and high‐density SSR linkage maps that are not yet available. Polymorphism in the set of reported SSR markers has been found to be low in safflower (Kadirvel et al., 2017; Usha Kiran et al., 2017), which is a limitation for QTL mapping in a specific mapping population. Recently, next‐generation sequencing (NGS) technologies and bioinformatics tools have become very helpful in extracting more marker loci across genomes (Bowers et al., 2016; Xia et al., 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Polymorphism in the set of reported SSR markers has been found to be low in safflower (Kadirvel et al, 2017;Usha Kiran et al, 2017), which is a limitation for QTL mapping in a specific mapping population.…”

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Genetic mapping reveals a major QTL associated with tolerance to the aphid, Uroleucon compositae (Theobald) in safflower (Carthamus tinctorius)

et al. 2021

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Safflower, an important oilseed crop species under the Asteraceae family, is grown for edible oil and various other industrial uses, including the preparation of natural dyes, cosmetics, traditional medicines, etc. Globally, more than 20 countries covering about one million ha are involved in the production of about 0.7 million tons of safflower. Kazakhstan, Russia, India, the United States, and Mexico are the major producers of safflower (FAOSTAT, 2017). In India, safflower is cultivated in about 50,000 ha predominantly under rain-fed situations during the winter season. Productivity of safflower (~700 kg/ha) in India is lower than in other countries. In India, safflower productivity is mainly affected by disease (wilt caused by Fusarium oxysporum f. sp. carthami and leaf blight caused by Alternaria carthami), insect pest [aphid, Uroleucon compositae (Theobald)] and moisture stress (Nimbkar, 2008).Uroleucon compositae (Hemiptera: Aphididae) are small, shiny black aphids, which suck phloem sap from various parts of safflower including stem, leaves, and capitula. They colonize on the slender stem during the elongation stage (40-45 days after sowing) and proliferate by viviparous mode of reproduction (Bhumannavar & Thontadarya, 1983). Damage symptoms manifest as yellowing

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Genetic distinctiveness of safflower cultivars of India and Mexico as revealed by SSR markers

Cited by 8 publications

References 45 publications

Distinctness of Brazilian common bean cultivars with carioca and black grain by means of morphoagronomic and molecular descriptors

Distinctness of Brazilian common bean cultivars with carioca and black grain by means of morphoagronomic and molecular descriptors

Occurrence of simple sequence repeats in cDNA sequences of safflower (Carthamus tinctorius) reveals the importance of SSR-containing genes for cell biology and dynamic response to environmental cues

Genetic mapping reveals a major QTL associated with tolerance to the aphid, Uroleucon compositae (Theobald) in safflower (Carthamus tinctorius)

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