Genetic dissection of the shoot and root ionomes of Brassica napus grown with contrasting phosphate supplies

Abstract

Background and Aims Mineral elements have many essential and beneficial functions in plants. Phosphorus (P) deficiency can result in changes in the ionomes of plant organs. The aims of this study were to characterize the effects of P supply on the ionomes of shoots and roots, and to identify chromosomal quantitative trait loci (QTLs) for shoot and root ionomic traits, as well as those affecting the partitioning of mineral elements between shoot and root in Brassica napus grown with contrasting P supplies. Methods Shoot and root concentrations of 11 mineral elements (B, Ca, Cu, Fe, K, Mg, Mn, Na, P, S and Zn) were investigated by inductively coupled plasma optical emission spectrometry (ICP-OES) in a Brassica napus double haploid population grown at an optimal (OP) and a low phosphorus supply (LP) in an agar system. Shoot, root and plant contents, and the partitioning of mineral elements between shoot and root were calculated. Key Results The tissue concentrations of B, Ca, Cu, K, Mg, Mn, Na, P and Zn were reduced by P starvation, while the concentration of Fe was increased by P starvation in the BnaTNDH population. A total of 133 and 123 QTLs for shoot and root ionomic traits were identified at OP and LP, respectively. A major QTL cluster on chromosome C07 had a significant effect on shoot Mg and S concentrations at LP and was narrowed down to a 2.1 Mb region using an advanced backcross population. Conclusions The tissue concentration and partitioning of each mineral element was affected differently by P starvation. There was a significant difference in mineral element composition between shoots and roots. Identification of the genes underlying these QTLs will enhance our understanding of processes affecting the uptake and partitioning of mineral elements in Brassica napus.

Silicon modifies leaf nutriome and improves growth of oak seedlings exposed to phosphorus deficiency and Phytophthora plurivora infection

“…In fact, we can not explain the Simediated changes of the majority of the examined nutrients under the three stresses. For instance, leaf B (Figure 7A) was also found to increase in different species under P deficiency (Baxter et al, 2008;Wang et al, 2020;Meng et al, 2021), which was further confirmed by increased expression of B transporters (Wang et al, 2018). In our work, the addition of Si under individual stress conditions tended to restore B but also P accumulation levels closer to the control.…”

“…For instance, Baxter et al (2008) found that low P caused an increase of B, Fe, and Zn, and a decrease of Co and Cu leaf concentrations in Arabidopsis. Wang et al (2020) likewise reported that P starvation resulted in an increase of B, Fe, S, and Zn, and a concomitant decrease of Ca, Cu, K, and Mg in oilseed rape. On the contrary, Courbet et al (2021) observed a P-deficiency caused increase in the uptake of Si in wheat, and a decrease in the uptake of majority of the nutrients in both oilseed rape and wheat.…”

“…So far, the response of leaf ionomics to P deficiency has not been unequivocally established (except for decrease in P concentrations). In addition, very little is known on molecular determinants of alterations in ionome caused by P supply fluctuations (Wang et al, 2020). For instance, Baxter et al (2008) found that low P caused an increase of B, Fe, and Zn, and a decrease of Co and Cu leaf concentrations in Arabidopsis.…”

Genome‐wide association mapping identifies HvNIP2;2/HvLsi6 accounting for efficient boron transport in barley

“…Natural variation is the basis for crop improvement and the identification of key genes underlying complex agronomic traits (Jia et al, 2020; Jia, Giehl, et al, 2019; Kuhlmann et al, 2020; Schnurbusch et al, 2010; Sutton et al, 2007). Like observed in other plant species, such as rice (C. C. Wang, Tang, et al, 2020; Yang et al, 2018), wheat (Jamjod et al, 2004), and Brassica napus (W. Wang, Ding, et al, 2020), grain B concentrations in a panel of 135 field‐grown barley accession lines differed by up to threefold (Figure 1, Table S1). Grain B accumulation depends on multiple transport steps involving (1) B uptake by the root, (2) root‐to‐shoot translocation, (3) B allocation to various tissues, and (4) B loading into grains.…”

Advances in “Omics” Approaches for Improving Toxic Metals/Metalloids Tolerance in Plants

“…Besides, ZmHMA2/3 and ZmMOT1 were also proposed to cause genetic variation of Cd and Mo contents in maize shoots. Another ionome study targeting shoots and roots of Brassica species identified a total of 133 and 123 QTLs under optimal and low P (OP/LP) conditions ( Wang et al, 2020e ). These QTL clusters are predicted to reveal the uptake and transport mechanisms of mineral elements.…”