Genetic dissection of the powdery mildew resistance in wheat breeding line LS5082 using BSR-Seq

Wu, Liru; Zhu, Tong; He, Huagang; Cao, Xinyou; Li, Haosheng; Xu, Hongxing; Jia, Mengshu; Zhang, Lipei; Song, Jiancheng; Mirzaghaderi, Ghader; Liu, Cheng; Ma, Pengtao

doi:10.1016/j.cj.2021.12.008

Cited by 17 publications

(11 citation statements)

References 58 publications

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“…The strong LD makes it challenging to discover the causal genes of the association, which could be a single or multiple resistance genes. Indeed, many candidate Pm genes have been described in this genomic interval, including Pm51 (57), Pm63 (60), PmKN0816 (62), PmLS5082 (63), PmQ (64) and pmYN99102 (66). Accessions that likely contained the causal gene were predominantly from Iran and the USA.…”

Section: Resultsmentioning

confidence: 99%

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A diverse panel of 755 bread wheat accessions harbors untapped genetic diversity in landraces and reveals novel genetic regions conferring powdery mildew resistance

Leber

Heuberger

Widrig

et al. 2023

Preprint

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Wheat breeding for disease resistance relies on the availability and use of diverse genetic resources. More than 800,000 wheat accessions are globally conserved in gene banks, but they are mostly uncharacterized for the presence of resistance genes and their potential for agriculture. Based on the selective reduction of previously assembled collections for allele mining, we assembled a trait-customized panel of 755 geographically diverse bread wheat accessions with a focus on landraces, called the LandracePLUS panel. Population structure analysis of this panel based on the TaBW35K SNP array revealed an increased genetic diversity compared to high-quality sequenced wheat accessions and 632 landraces of an earlier study. The additional diversity mostly originated from Turkish, Iranian and Pakistani landraces. We characterized the LandracePLUS panel for resistance to ten diverse isolates of the fungal pathogen powdery mildew. Performing genome-wide association studies and dividing the panel further by a targeted sub-setting approach for accessions with unique resistance patterns and distinct geographical origin, we detected several known and already cloned genes, including thePm2agene. In addition, we identified 17 putatively novel powdery mildew resistance loci that represent useful sources for resistance breeding and for research on the mildew-wheat pathosystem. Our study shows the value of assembling trait-customized collections and utilizing a diverse range of pathogen races, to detect novel loci that can be integrated in breeding programs. It further highlights the importance of integrating landraces of different geographical origin into future diversity studies.

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Section: Resultsmentioning

confidence: 99%

“…This introgression is potentially present in the LandracePLUS panel. Many candidate Pm genes have already been described in this genomic interval, including Pm51 (Zhan et al 2014), Pm63 (Tan et al 2019), PmKN0816 (Wang et al 2021), PmLS5082 (Wu et al 2022), PmQ (Li et al 2020b), pmYN99102 (Mu et al 2022) and PmCG15-009 (Zhang et al 2023).…”

Section: Resultsmentioning

confidence: 99%

A diverse panel of 755 bread wheat accessions harbors untapped genetic diversity in landraces and reveals novel genetic regions conferring powdery mildew resistance

Leber

Heuberger

Widrig

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Due to the ease of selection and phenotypic evaluation, most disease resistance studies have focused on ASR genes, which are known as qualitative or race-specific resistance genes ( Wu et al., 2022 ). However, overuse of single race-specific resistance genes can lead to the rapid evolution of new virulent Bgt races and consequent massive economic losses ( Singh et al., 2016 ).…”

Section: Resultsmentioning

confidence: 99%

Identification of the major QTL QPm.cas-7D for adult plant resistance to wheat powdery mildew

Liu

Han²,

Gu³

et al. 2022

Front. Plant Sci.

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Developing effective and durable host plant resistance is crucial for controlling powdery mildew, a devastating disease caused by Blumeria graminis f. sp. tritici (Bgt). In the present study, we dissected the genetic basis of the adult plant resistance to powdery mildew using a recombinant inbred line (RIL) composed of 176 F9 RILs population derived from a cross between PuBing 3228 (P3228) and susceptible cultivar Gao 8901. P3228 exhibits stable adult-plant resistance to powdery mildew in the field over consecutive years. We identified two QTLs on chromosomes 7DS (QPm.cas-7D) and 1AL (QPm.cas-1A) contributed by P3228, and one QTL on 3DS (QPm.cas-3D) contributed by Gao 8901, which could explain 65.44%, 3.45%, and 2.18% of the phenotypic variances, respectively. By analyzing the annotated genes in the 1.168 Mb physical interval of the major QTL QPm.cas-7D, we locked a previously cloned adult-plant resistance gene Pm38 that was most probably the candidate gene of QPm.cas-7D. Sequence alignment analysis revealed that the candidate gene of QPm.cas-7D in P3228 was identical to the reported Pm38 sequence. Two haplotypes QPm-7D-R and QPm-7D-S were identified in the whole Pm38 genomic regions between P3228 and Gao 8901. To apply QPm.cas-7D in wheat breeding, we developed a kompetitive allele-specific PCR (KASP) marker Kasp5249 that is closely linked with these haplotypes. It is worth mentioning that the QPm-7D-R haplotype significantly decreased TKW and underwent negative selection for higher yields in China wheat breeding. In this study, we identified a major QTL QPm.cas-7D and revealed the relationship between its resistance and yield, which could be beneficial for further applications in wheat disease resistance and high-yield breeding.

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“…Over 60 loci for Pm resistance have been described in wheat and its relatives ( Hewitt et al., 2021 ). To date, 12 PM resistance genes have been cloned and are listed in Table 2 ( Wu et al., 2022 ). Most cloned R genes are NBS-LRR type, and the rest are kinases.…”

Section: Powdery Mildewmentioning

confidence: 99%

Important wheat diseases in the US and their management in the 21st century

Singh

Chhabra²,

Raza³

et al. 2023

Front. Plant Sci.

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Wheat is a crop of historical significance, as it marks the turning point of human civilization 10,000 years ago with its domestication. Due to the rapid increase in population, wheat production needs to be increased by 50% by 2050 and this growth will be mainly based on yield increases, as there is strong competition for scarce productive arable land from other sectors. This increasing demand can be further achieved using sustainable approaches including integrated disease pest management, adaption to warmer climates, less use of water resources and increased frequency of abiotic stress tolerances. Out of 200 diseases of wheat, 50 cause economic losses and are widely distributed. Each year, about 20% of wheat is lost due to diseases. Some major wheat diseases are rusts, smut, tan spot, spot blotch, fusarium head blight, common root rot, septoria blotch, powdery mildew, blast, and several viral, nematode, and bacterial diseases. These diseases badly impact the yield and cause mortality of the plants. This review focuses on important diseases of the wheat present in the United States, with comprehensive information of causal organism, economic damage, symptoms and host range, favorable conditions, and disease management strategies. Furthermore, major genetic and breeding efforts to control and manage these diseases are discussed. A detailed description of all the QTLs, genes reported and cloned for these diseases are provided in this review. This study will be of utmost importance to wheat breeding programs throughout the world to breed for resistance under changing environmental conditions.

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Genetic dissection of the powdery mildew resistance in wheat breeding line LS5082 using BSR-Seq

Cited by 17 publications

References 58 publications

A diverse panel of 755 bread wheat accessions harbors untapped genetic diversity in landraces and reveals novel genetic regions conferring powdery mildew resistance

A diverse panel of 755 bread wheat accessions harbors untapped genetic diversity in landraces and reveals novel genetic regions conferring powdery mildew resistance

Identification of the major QTL QPm.cas-7D for adult plant resistance to wheat powdery mildew

Important wheat diseases in the US and their management in the 21st century

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