Type 1 cannabinoid (CB ) receptors are widely distributed in the brain. Their physiological roles depend on their distribution pattern, which differs remarkably among cell types. Hence, subcellular compartments with little but functionally relevant CB receptors can be overlooked, fostering an incomplete mapping. To overcome this, knockin mice with cell-type-specific rescue of CB receptors have emerged as excellent tools for investigating CB receptors' cell-type-specific localization and sufficient functional role with no bias. However, to know whether these rescue mice maintain endogenous CB receptor expression level, detailed anatomical studies are necessary. The subcellular distribution of hippocampal CB receptors of rescue mice that express the gene exclusively in dorsal telencephalic glutamatergic neurons (Glu-CB -RS) or GABAergic neurons (GABA-CB -RS) was studied by immunoelectron microscopy. Results were compared with conditional CB receptor knockout lines. As expected, CB immunoparticles appeared at presynaptic plasmalemma, making asymmetric and symmetric synapses. In the hippocampal CA1 stratum radiatum, the values of the CB receptor-immunopositive excitatory and inhibitory synapses were Glu-CB -RS, 21.89% (glutamatergic terminals); 2.38% (GABAergic terminals); GABA-CB -RS, 1.92% (glutamatergic terminals); 77.92% (GABAergic terminals). The proportion of CB receptor-immunopositive excitatory and inhibitory synapses in the inner one-third of the dentate molecular layer was Glu-CB -RS, 53.19% (glutamatergic terminals); 2.30% (GABAergic terminals); GABA-CB -RS, 3.19% (glutamatergic terminals); 85.07% (GABAergic terminals). Taken together, Glu-CB -RS and GABA-CB -RS mice show the usual CB receptor distribution and expression in hippocampal cell types with specific rescue of the receptor, thus being ideal for in-depth anatomical and functional investigations of the endocannabinoid system. J. Comp. Neurol. 525:302-318, 2017. © 2016 Wiley Periodicals, Inc.