Genetic dissection of maize seedling traits in an IBM Syn10 DH population under the combined stress of lead and cadmium

Hou, Fengxia; Zhou, Xun; Liu, Peng; Zou, Chaoying; Lübberstedt, Thomas; Pan, Guangtang; Ma, Langlang; Shen, Yaou

doi:10.1007/s00438-021-01800-2

Cited by 11 publications

(7 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among these 542 DEGs, 9 were related to ion, metal, or heavy metal transport. In addition, Zm00001d033564 , Zm00001d048731 , and Zm00001d034571 were the candidate genes of QTL mapping of root diameter, perimeter, and bushiness in maize seedling under Cd stress, respectively [ 48 ]. Furthermore, 12 DEGs were identified both in XNY22-CHS-8 vs. XNY22-YA-8 and XNY22-CHS-24 vs. XNY22-YA-24 comparison groups ( Figure 2 B), and seven DEGs were identified in XNY27-CHS-8 vs. XNY27-YA-8 and XNY27-CHS-24 vs. XNY27-YA-24 comparison groups ( Figure 2 C, Supplemental Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

“…Linkage mapping and GWAS have become effective strategies to reveal the genetic basis of complex agronomic traits. However, few studies have been performed on the genetic architecture of the traits related to Cd stress [ 48 , 49 , 50 , 51 ]. Meanwhile, several transcriptomics studies about maize roots response to Cd stress have been already published [ 52 , 53 , 54 , 55 , 56 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

WGCNA Analysis Revealed the Hub Genes Related to Soil Cadmium Stress in Maize Kernel (Zea mays L.)

Zhang

Luo

et al. 2022

Genes

View full text Add to dashboard Cite

Soil contamination by heavy metals has become a prevalent topic due to their widespread release from industry, agriculture, and other human activities. Great progress has been made in elucidating the uptake and translocation of cadmium (Cd) accumulation in rice. However, there is still little known about corresponding progress in maize. In the current study, we performed a comparative RNA-Seq-based approach to identify differentially expressed genes (DEGs) of maize immature kernel related to Cd stress. In total, 55, 92, 22, and 542 DEGs responsive to high cadmium concentration soil were identified between XNY22-CHS-8 vs. XNY22-YA-8, XNY22-CHS-24 vs. XNY22-YA-24, XNY27-CHS-8 vs. XNY27-YA-8, and XNY27-CHS-24 vs. XNY27-YA-24, respectively. The weighted gene co-expression network analysis (WGCNA) categorized the 9599 Cd stress-responsive hub genes into 37 different gene network modules. Combining the hub genes and DEGs, we obtained 71 candidate genes. Gene Ontology (GO) enrichment analysis of genes in the greenyellow module in XNY27-YA-24 and connectivity genes of these 71 candidate hub genes showed that the responses to metal ion, inorganic substance, abiotic stimulus, hydrogen peroxide, oxidative stress, stimulus, and other processes were enrichment. Moreover, five candidate genes that were responsive to Cd stress in maize kernel were detected. These results provided the putative key genes and pathways to response to Cd stress in maize kernel, and a useful dataset for unraveling the underlying mechanism of Cd accumulation in maize kernel.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

WGCNA Analysis Revealed the Hub Genes Related to Soil Cadmium Stress in Maize Kernel (Zea mays L.)

Zhang

Luo

et al. 2022

Genes

View full text Add to dashboard Cite

show abstract

“…showed that the root length and surface area were significantly inhibited under Pb stress in Elsholtzia argyi and Elsholtzia splendens ( Peng et al., 2005 ). Pb stress also inhibited lateral root formation by affecting root diameter and volume ( Hou et al., 2021 ). Currently, root dry weight has thereby been widely considered a reliable indicator for evaluating plant tolerance to abiotic stresses during early growth stages ( Pour-Aboughadareh et al., 2020 ; Ma et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…The significance threshold was set as p = 0.05 according to the previous study (Ma et al, 2022). Haploview software (http://www.broad.mit.edu/mpg/haploview) was used to calculate the LD between the markers (Hou et al, 2021). For each gene, haplotypes were identified according to these traitassociated SNPs and a t-test was used to analyze significance of BSH-LTC between contrasting haplotypes.…”

Section: Gene-based Association Analysismentioning

confidence: 99%

Association mapping uncovers maize ZmbZIP107 regulating root system architecture and lead absorption under lead stress

Hou

Zhang

et al. 2022

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Lead (Pb) is a highly toxic contaminant to living organisms and the environment. Excessive Pb in soils affects crop yield and quality, thus threatening human health via the food chain. Herein, we investigated Pb tolerance among a maize association panel using root bushiness (BSH) under Pb treatment as an indicator. Through a genome-wide association study of relative BSH, we identified four single nucleotide polymorphisms (SNPs) and 30 candidate genes associated with Pb tolerance in maize seedlings. Transcriptome analysis showed that four of the 30 genes were differentially responsive to Pb treatment between two maize lines with contrasting Pb tolerance. Among these, the ZmbZIP107 transcription factor was confirmed as the key gene controlling maize tolerance to Pb by using gene-based association studies. Two 5’ UTR_variants in ZmbZIP107 affected its expression level and Pb tolerance among different maize lines. ZmbZIP107 protein was specifically targeted to the nucleus and ZmbZIP107 mRNA showed the highest expression in maize seedling roots among different tissues. Heterologous expression of ZmbZIP107 enhanced rice tolerance to Pb stress and decreased Pb absorption in the roots. Our study provided the basis for revelation of the molecular mechanism underlying Pb tolerance and contributed to cultivation of Pb-tolerant varieties in maize.

show abstract

“…Nevertheless, the delivered multiple insect resistance physical map should contribute to the possible enhancement of combined insect resistance in maize. Hou et al [125] used an IBM Syn10 DH population [126] to detect the quantitative trait loci (QTL) for a combined lead and cadmium tolerance by linkage mapping in maize seedlings contributing to functional gene identification and molecular breeding for improving heavy metal tolerance.…”

Section: Natural Genetic Variationmentioning

confidence: 99%

Genetic Approaches to Enhance Multiple Stress Tolerance in Maize

Malenica

Dunić

Vukadinović

et al. 2021

Genes

View full text Add to dashboard Cite

The multiple-stress effects on plant physiology and gene expression are being intensively studied lately, primarily in model plants such as Arabidopsis, where the effects of six stressors have simultaneously been documented. In maize, double and triple stress responses are obtaining more attention, such as simultaneous drought and heat or heavy metal exposure, or drought in combination with insect and fungal infestation. To keep up with these challenges, maize natural variation and genetic engineering are exploited. On one hand, quantitative trait loci (QTL) associated with multiple-stress tolerance are being identified by molecular breeding and genome-wide association studies (GWAS), which then could be utilized for future breeding programs of more resilient maize varieties. On the other hand, transgenic approaches in maize have already resulted in the creation of many commercial double or triple stress resistant varieties, predominantly weed-tolerant/insect-resistant and, additionally, also drought-resistant varieties. It is expected that first generation gene-editing techniques, as well as recently developed base and prime editing applications, in combination with the routine haploid induction in maize, will pave the way to pyramiding more stress tolerant alleles in elite lines/varieties on time.

show abstract

Genetic dissection of maize seedling traits in an IBM Syn10 DH population under the combined stress of lead and cadmium

Cited by 11 publications

References 69 publications

WGCNA Analysis Revealed the Hub Genes Related to Soil Cadmium Stress in Maize Kernel (Zea mays L.)

WGCNA Analysis Revealed the Hub Genes Related to Soil Cadmium Stress in Maize Kernel (Zea mays L.)

Association mapping uncovers maize ZmbZIP107 regulating root system architecture and lead absorption under lead stress

Genetic Approaches to Enhance Multiple Stress Tolerance in Maize

Contact Info

Product

Resources

About