Genetic Dissection of Histidine Biosynthesis in Arabidopsis

Muralla, Rosanna; Sweeney, Colleen; Stepansky, Asya; Leustek, Thomas; Meinke, David W.

doi:10.1104/pp.107.096511

Cited by 75 publications

(92 citation statements)

References 53 publications

(56 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As would be expected, His is required throughout plant growth and development (Muralla et al, 2007). The most common phenotype in null Arabidopsis mutants defective in one of the five non-redundant HISN genes is seed abortion at the preglobular stage of embryo development, which can be rescued by supplying heterozygous plants with exogenous His (Muralla et al, 2007;Petersen et al, 2010).…”

Section: Introductionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 97%

“…The most common phenotype in null Arabidopsis mutants defective in one of the five non-redundant HISN genes is seed abortion at the preglobular stage of embryo development, which can be rescued by supplying heterozygous plants with exogenous His (Muralla et al, 2007;Petersen et al, 2010). These data indicate that endogenous His concentrations in the surrounding heterozygous maternal tis- sues are insufficient to support embryonic development of null hisn mutants (Muralla et al, 2007). Homozygous null mutants obtained by His feeding of heterozygotes are able to germinate and grow, provided that His is available in the growth medium, however mature plants display a range of phenotypes including reduced apical dominance and fertility (Muralla et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Histidine Biosynthesis

Ingle

2011

The Arabidopsis Book

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Histidine Biosynthesis

Ingle

2011

The Arabidopsis Book

View full text Add to dashboard Cite

“…Embryos were observed using differential interference contrast microscopy (BX-60, Olympus, Tokyo) on samples mounted with Hoyerʼs solution (Muralla et al 2007) to make them clear.…”

Section: Methodsmentioning

confidence: 99%

T-DNA Insertion in the <i>CLS</i> Gene for Cardiolipin Synthase Affects Development of <i>Arabidopsis thaliana</i>

Katayama

Wada

2012

cytologia

View full text Add to dashboard Cite

Summary Cardiolipin (CL) is widely distributed in various prokaryotes and eukaryotes as a membrane phospholipid. In eukaryotes, it is localized in the inner membrane and at the contact sites between the inner and outer membranes of mitochondria. CL is suggested to be involved in many mitochondrial functions. We previously identified the CLS gene for CL synthase in Arabidopsis thaliana and showed it to be a useful tool for understanding the physiological roles of CL in higher plants. In this study, we isolated homozygous cls mutants (cls-1 and cls-2) of A. thaliana, in which the CLS gene was disrupted by T-DNA insertion, by observing the development of embryos obtained after self-fertilization of heterozygous cls mutants. Both embryogenesis and growth of the homozygous cls mutants were substantially retarded relative to wild type, and additional phenotypes were observed, namely slow root growth, an abnormal veining pattern in cotyledons, and a low yield of seed production. The delayed embryogenesis and growth of homozygous cls mutants were recovered by introduction of CLS into the mutants. These findings demonstrate that CLS plays important roles in development of A. thaliana, presumably due to the biosynthesis of CL in the mitochondria. Biological membranes required for compartmentalization of cells and organelles are mainly composed of lipids and proteins. Lipids provide a hydrophobic environment in the membranes and form a bilayer that is a fundamental structure of the membranes. The lipid compositions of various biological membranes, such as the mitochondrial and plasma membranes, are different, and there are many lipid classes in a cell. Cardiolipin (CL) is widely distributed in various prokaryotes and mitochondria of eukaryotes as a membrane phospholipid. In eukaryotes, it comprises approximately 10% of the mitochondrial membrane lipids and mainly exists in the inner membrane and the contact sites between the outer and inner membranes of the mitochondria (Frentzen and Griebau 1994). Key wordsAs shown in Fig. 1, CL is synthesized from phosphatidic acid (PA), which is synthesized from glycerol 3-phosphate by a 2-step acylation. PA is converted into CDP-diacylglycerol (CDP-DAG) by CDP-DAG synthase, which transfers the CMP moiety from CTP to PA. The synthesized CDP-DAG reacts with glycerol 3-phosphate to produce phosphatidylglycerophosphate (PGP) and CMP in a reaction catalyzed by PGP synthase. Then, the resulting PGP is converted into phosphatidylglycerol (PG) by dephosphorylation catalyzed by PGP phosphatase. In the final step in the biosynthesis of CL, CL synthase (CLS) transfers a phosphatidyl group from CDP-DAG to PG to produce

show abstract

“…It is not entirely clear from our analysis which tissue of the developing seed is primarily impaired by the lack of Arg and causes growth arrest, but the embryo is most likely affected. Different amino acid biosynthesis mutants showing embryo lethal phenotypes have been described, including mutants in His (Muralla et al, 2007), Lys (Song et al, 2004;Hudson et al, 2006), and Pro biosynthesis genes (Székely et al, 2008). Meinke (1991) and Bryant et al (2011) also pointed out that embryonic development is often interrupted in mutants defective in essential housekeeping genes, including those of amino acid biosynthesis.…”

Section: Tup5 Is Required For Gametogenesis and Seed Developmentmentioning

confidence: 99%

The ArabidopsisTUMOR PRONE5Gene Encodes an Acetylornithine Aminotransferase Required for Arginine Biosynthesis and Root Meristem Maintenance in Blue Light

et al. 2013

View full text Add to dashboard Cite

Arginine is an essential amino acid necessary for protein synthesis and is also a nitrogen storage compound. The genes encoding the enzymes of arginine biosynthesis in plants are not well characterized and have mainly been predicted from homologies to bacterial and fungal genes. We report the cloning and characterization of the TUMOR PRONE5 (TUP5) gene of Arabidopsis (Arabidopsis thaliana) encoding an acetylornithine aminotransferase (ACOAT), catalyzing the fourth step of arginine biosynthesis. The free arginine content was strongly reduced in the chemically induced recessive mutant tup5-1, root growth was restored by supplementation with arginine and its metabolic precursors, and a yeast (Saccharomyces cerevisiae) ACOAT mutant was complemented by TUP5. Two null alleles of TUP5 caused a reduced viability of gametes and embryo lethality, possibly caused by insufficient Arg supply from maternal tissue. TUP5 expression is positively regulated by light, and a TUP5-green fluorescent protein was localized in chloroplasts. tup5-1 has a unique light-dependent short root phenotype. Roots of light-grown tup5-1 seedlings switch from indeterminate growth to determinate growth with arresting cell production and an exhausted root apical meristem. The inhibitory activity was specific for blue light, and the inhibiting light was perceived by the root. Thus, tup5-1 reveals a novel role of amino acids and blue light in regulating root meristem function.

show abstract

Genetic Dissection of Histidine Biosynthesis in Arabidopsis

Cited by 75 publications

References 53 publications

Histidine Biosynthesis

Histidine Biosynthesis

T-DNA Insertion in the <i>CLS</i> Gene for Cardiolipin Synthase Affects Development of <i>Arabidopsis thaliana</i>

The ArabidopsisTUMOR PRONE5Gene Encodes an Acetylornithine Aminotransferase Required for Arginine Biosynthesis and Root Meristem Maintenance in Blue Light

Contact Info

Product

Resources

About