Genetic Dissection of Heat Stress Tolerance in Faba Bean (Vicia faba L.) Using GWAS

Abstract

Heat waves are expected to become more frequent and intense, which will impact faba bean cultivation globally. Conventional breeding methods are effective but take considerable time to achieve breeding goals, and, therefore, the identification of molecular markers associated with key genes controlling heat tolerance can facilitate and accelerate efficient variety development. We phenotyped 134 accessions in six open field experiments during summer seasons at Terbol, Lebanon, at Hudeiba, Sudan, and at Central Ferry, WA, USA from 2015 to 2018. These accessions were genotyped using genotyping by sequencing (GBS), and 10,794 high quality single nucleotide polymorphisms (SNPs) were discovered. These accessions were clustered in one diverse large group, although several discrete groups may exist surrounding it. Fifteen lines belonging to different botanical groups were identified as tolerant to heat. SNPs associated with heat tolerance using single-trait (ST) and multi-trait (MT) genome-wide association studies (GWASs) showed 9 and 11 significant associations, respectively. Through the annotation of the discovered significant SNPs, we found that SNPs from transcription factor helix–loop–helix bHLH143-like S-adenosylmethionine carrier, putative pentatricopeptide repeat-containing protein At5g08310, protein NLP8-like, and photosystem II reaction center PSB28 proteins are associated with heat tolerance.

Genetic analysis of global faba bean diversity, agronomic traits and selection signatures

“…Few studies have been performed to identify QTLs of agronomically important traits in faba bean (Khazaei et al 2021 ). Although a couple of recent studies have performed GWAS on unrelated and diverse faba bean germplasm (Maalouf et al 2022 ; Abou-Khater et al 2022 ), most of the published studies have relied on biparental populations, limiting the amount of genetic variation studied as compared to a MAGIC population. Here, we use GWAS to identify 238 significant marker-trait associations linked to 12 agronomic important traits.…”

Agronomic Performance of Broomrape Resistant and Susceptible Faba Bean Accession

“…Still, even for the early sowings performed in our trials, damage due to high temperatures at the reproductive stage was noticed in the negative association of grain yield with maximum temperatures. Some sources of tolerance to heat have recently [52] been identified, together with associated genetic markers to make selections in early generations. Whilst waiting for heat-tolerant cultivars to be released, escaping the heat-effects by early sowing is the only method available for farmers, which also helps to avoid terminal drought [2,13].…”

Advancement in Genomics and Molecular Marker Technologies for Breeding of Faba Bean with Low Vicine-convicine Content: A Review

“…Though initially several genetic maps were developed with the help of morphological characteristics, isozymes, seed protein genes and random amplified polymorphic DNA (RAPD) markers, later faba bean genetic studies and breeding have been enriched due to development of expressed sequence tags (ESTs), microsatellites or simple sequence repeats (SSRs), EST-SSRs, single nucleotide polymorphisms (SNPs) and Kompetitive Allele Specific PCR (KASP) markers (Maalouf et al, 2022;Khazaei et al, 2021;Zanotto et al, 2020;Abuzayed, 2019;Khazaei et al, 2017;Kaur et al, 2014a;). Several mapping populations were developed for flowering, yield-related traits and plant architecture (Avila et al, 2017;Cruz-Izquierdo et al, 2012), biochemical and morphological traits (Ramsay et al, 1995), seed weight (Vaz Patto et al, 1999), drought adaptationrelated, morphological traits and vicine-convicine (Khazaei et al, 2015(Khazaei et al, , 2014a(Khazaei et al, , 2014b, rust resistance (Ijaz, 2018), rust, broomrape and ascochyta blight resistance (Román et al, 2004).…”