Genetic dissection of basal defence responsiveness in accessions of <i>Arabidopsis thaliana</i>

Ahmad, Shakoor; Hulten, Marieke van; Martin, J. L.; Pieterse, Corné M. J.; Wees, Saskia C.M. Van; Ton, Jurriaan

doi:10.1111/j.1365-3040.2011.02317.x

Cited by 46 publications

(31 citation statements)

References 92 publications

(194 reference statements)

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“…Consistent with this, we found that JA induces RGL3 expression to a substantially higher level in Can-0 and Sf-2 than in Col-0 and that the basal level of primary JA-responsive gene transcripts is also higher and is induced to higher levels by JA in Sf-2 than in Col-0 (see Supplemental Figure 8 online). Interestingly, the Sf-2 accession was recently reported to be naturally more resistant to various pathogens, including the necrotrophic fungus Plectosphaerella cucumerina (Ahmad et al, 2011). Results from our study raise the possibility that DELLA gene duplication events (that probably occurred after the divergence between dicots and monocots) increase the plasticity and the adaptation of plants to their surrounding growth conditions.…”

Section: Discussionmentioning

confidence: 53%

The Arabidopsis DELLA RGA-LIKE3 Is a Direct Target of MYC2 and Modulates Jasmonate Signaling Responses

et al. 2012

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Gibberellins (GAs) are plant hormones involved in the regulation of plant growth in response to endogenous and environmental signals. GA promotes growth by stimulating the degradation of nuclear growth-repressing DELLA proteins. In Arabidopsis thaliana, DELLAs consist of a small family of five proteins that display distinct but also overlapping functions in repressing GA responses. This study reveals that DELLA RGA-LIKE3 (RGL3) protein is essential to fully enhance the jasmonate (JA)-mediated responses. We show that JA rapidly induces RGL3 expression in a CORONATINE INSENSITIVE1 (COI1)-and JASMONATE INSENSITIVE1 (JIN1/MYC2)-dependent manner. In addition, we demonstrate that MYC2 binds directly to RGL3 promoter. Furthermore, we show that RGL3 (like the other DELLAs) interacts with JA ZIM-domain (JAZ) proteins, key repressors of JA signaling. These findings suggest that JA/MYC2-dependent accumulation of RGL3 represses JAZ activity, which in turn enhances the expression of JA-responsive genes. Accordingly, we show that induction of primary JA-responsive genes is reduced in the rgl3-5 mutant and enhanced in transgenic lines overexpressing RGL3. Hence, RGL3 positively regulates JA-mediated resistance to the necrotroph Botrytis cinerea and susceptibility to the hemibiotroph Pseudomonas syringae. We propose that JA-mediated induction of RGL3 expression is of adaptive significance and might represent a recent functional diversification of the DELLAs.

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Section: Discussionmentioning

confidence: 53%

The Arabidopsis DELLA RGA-LIKE3 Is a Direct Target of MYC2 and Modulates Jasmonate Signaling Responses

et al. 2012

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“…PAMPs are generally highly conserved among diverse microbial species (Boyd et al, 2013). Two RLK examples have been described which confer non-host resistance to Psph in A. thaliana , including FLS2 which enables detection of flagellin (Forsyth et al, 2010; Ahmad et al, 2011). In P. vulgaris , RLKs on Pv01 and Pv08 have been identified as candidates for race-specific resistance to anthracnose caused by Colletotrichum lindemuthianum (Burt et al, 2015; Zuiderveen et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Linkage and Association Mapping of Halo Blight Resistance in Common Bean to Race 6 of the Globally Important Bacterial Pathogen

Tock

Fourie²,

Walley

et al. 2017

Front. Plant Sci.

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Pseudomonas syringae pv. phaseolicola (Psph) Race 6 is a globally prevalent and broadly virulent bacterial pathogen with devastating impact causing halo blight of common bean (Phaseolus vulgaris L.). Common bean lines PI 150414 and CAL 143 are known sources of resistance against this pathogen. We constructed high-resolution linkage maps for three recombinant inbred populations to map resistance to Psph Race 6 derived from the two common bean lines. This was complemented with a genome-wide association study (GWAS) of Race 6 resistance in an Andean Diversity Panel of common bean. Race 6 resistance from PI 150414 maps to a single major-effect quantitative trait locus (QTL; HB4.2) on chromosome Pv04 and confers broad-spectrum resistance to eight other races of the pathogen. Resistance segregating in a Rojo × CAL 143 population maps to five chromosome arms and includes HB4.2. GWAS detected one QTL (HB5.1) on chromosome Pv05 for resistance to Race 6 with significant influence on seed yield. The same HB5.1 QTL, found in both Canadian Wonder × PI 150414 and Rojo × CAL 143 populations, was effective against Race 6 but lacks broad resistance. This study provides evidence for marker-assisted breeding for more durable halo blight control in common bean by combining alleles of race-nonspecific resistance (HB4.2 from PI 150414) and race-specific resistance (HB5.1 from cv. Rojo).

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“…We found IRM1 expression to be suppressed in Col-0 upon aphid infestation whereas in WS it was initially induced, but suppressed afterwards. Such differences may result from the genetic differences among the two A. thaliana ecotypes in the basal defence to aphids [35].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of IRM1 Enhances Resistance to Aphids in Arabidopsis thaliana

et al. 2013

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Aphids are insects that cause direct damage to crops by the removal of phloem sap, but more importantly they spread devastating viruses. Aphids use their sophisticated mouthpart (i.e. stylet) to feed from the phloem sieve elements of the host plant. To identify genes that affect host plant resistance to aphids, we previously screened an Arabidopsis thaliana activation tag mutant collection. In such mutants, tagged genes are overexpressed by a strong 35S enhancer adjacent to the natural promoter, resulting in a dominant gain-of-function phenotype. We previously identified several of these mutants on which the aphid Myzus persicae showed a reduced population development compared with wild type. In the present study we show that the gene responsible for the phenotype of one of the mutants is At5g65040 and named this gene Increased Resistance to Myzus persicae 1 (IRM1). Overexpression of the cloned IRM1 gene conferred a phenotype identical to that of the original mutant. Conversely, an IRM1 knockout mutant promoted aphid population development compared to the wild type. We performed Electrical Penetration Graph analysis to investigate how probing and feeding behaviour of aphids was affected on plants that either overexpressed IRM1 or contained a knockout mutation in this gene. The EPG results indicated that the aphids encounter resistance factors while reaching for the phloem on the overexpressing line. This resistance mechanism also affected other aphid species and is suggested to be of mechanical nature. Interestingly, genetic variation for IRM1 expression in response to aphid attack was observed. Upon aphid attack the expression of IRM1 was initially (after 6 hours) induced in ecotype Wassilewskija followed by suppression. In Columbia-0, IRM1 expression was already suppressed six hours after the start of the infestation. The resistance conferred by the overexpression of IRM1 in A. thaliana trades off with plant growth.

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Genetic dissection of basal defence responsiveness in accessions of Arabidopsis thaliana

Cited by 46 publications

References 92 publications

The Arabidopsis DELLA RGA-LIKE3 Is a Direct Target of MYC2 and Modulates Jasmonate Signaling Responses

The Arabidopsis DELLA RGA-LIKE3 Is a Direct Target of MYC2 and Modulates Jasmonate Signaling Responses

Genome-Wide Linkage and Association Mapping of Halo Blight Resistance in Common Bean to Race 6 of the Globally Important Bacterial Pathogen

Overexpression of IRM1 Enhances Resistance to Aphids in Arabidopsis thaliana

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