Genetic Discrimination of Catharanthus roseus Cultivars by Pyrolysis Mass Spectrometry

Kim, Suk Weon; Kim, Jong Hyun; Liu, Jang Ryol

doi:10.1007/s12374-009-9059-1

Cited by 7 publications

(3 citation statements)

References 11 publications

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“…Among its many applications it can be mentioned that the Py-GC/MS has proven to be a very sensitive technique for identification and discrimination of paintings [13][14] and traces of vehicle tyres [15]. But it is not exclusive to art of forensic science, since it is widely used in the study and characterisation of all types of materials including polymers [16][17], various biomasses [18][19], food products [20][21][22] or algae [23], among others.…”

Section: Introductionmentioning

confidence: 99%

Thermogravimetric analysis and Py-GC/MS for discrimination of leather from different animal species and tanning processes

Bañón

García

Marcilla

2021

Journal of Analytical and Applied Pyrolysis

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Section: Introductionmentioning

confidence: 99%

Thermogravimetric analysis and Py-GC/MS for discrimination of leather from different animal species and tanning processes

Bañón

García

Marcilla

2021

Journal of Analytical and Applied Pyrolysis

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“…Efforts have been made to select for cultivars with various morphological traits or higher alkaloid contents [17]. Previous studies have characterized and differentiated the cultivars using approaches such as amplified fragment length polymorphisms (AFLP), random amplified polymorphic DNA (RAPD) [21], and chemotaxonomy [22]. However, since there are now over 100 cultivars of C .…”

Section: Introductionmentioning

confidence: 99%

The Complete Plastid Genome Sequence of Madagascar Periwinkle Catharanthus roseus (L.) G. Don: Plastid Genome Evolution, Molecular Marker Identification, and Phylogenetic Implications in Asterids

Chung

Chen

et al. 2013

PLoS ONE

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The Madagascar periwinkle ( Catharanthus roseus in the family Apocynaceae) is an important medicinal plant and is the source of several widely marketed chemotherapeutic drugs. It is also commonly grown for its ornamental values and, due to ease of infection and distinctiveness of symptoms, is often used as the host for studies on phytoplasmas, an important group of uncultivated plant pathogens. To gain insights into the characteristics of apocynaceous plastid genomes (plastomes), we used a reference-assisted approach to assemble the complete plastome of C . roseus , which could be applied to other C . roseus -related studies. The C . roseus plastome is the second completely sequenced plastome in the asterid order Gentianales. We performed comparative analyses with two other representative sequences in the same order, including the complete plastome of Coffea arabica (from the basal Gentianales family Rubiaceae) and the nearly complete plastome of Asclepias syriaca (Apocynaceae). The results demonstrated considerable variations in gene content and plastome organization within Apocynaceae, including the presence/absence of three essential genes (i.e., accD, clpP, and ycf1) and large size changes in non-coding regions (e.g., rps2-rpoC2 and IRb-ndhF). To find plastome markers of potential utility for Catharanthus breeding and phylogenetic analyses, we identified 41 C . roseus -specific simple sequence repeats. Furthermore, five intergenic regions with high divergence between C . roseus and three other euasterids I taxa were identified as candidate markers. To resolve the euasterids I interordinal relationships, 82 plastome genes were used for phylogenetic inference. With the addition of representatives from Apocynaceae and sampling of most other asterid orders, a sister relationship between Gentianales and Solanales is supported.

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“…PyMS has been widely applied to the discrimination of closely related microbial strains (Timmins et al 1998). Recently, PyMS is also used for the discrimination of plant calluses based on embryogenic capacity (Kim et al 2007) and taxonomic classification of higher plants (Kim et al 2004(Kim et al , 2007 and genetic discrimination of Catharanthus roseus cultivars (Kim et al 2009) as well.…”

Section: Introductionmentioning

confidence: 99%

A Rapid, Simple Method for the Determination of 6-Benzyladenine by Pyrolysis Mass Spectrometry

Kim

Liu

2009

J. Plant Biol.

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Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex material in a vacuum. PyMS was used for the quantitative determination of 6-benzyladenine (BA) supplemented to agar-solidified culture media (ASM) in this study. When subjected to PyMS, pure BA generated prominent fingerprint peaks. The peaks at m/z 68 and 123 were chosen for the quantitative measurement of BA because of the highest signal among those generated from pure BA and because of one of the highest masses among those with a prominent signal, respectively. To establish a standard curve for BA concentration in ASM, the combined peak intensity at m/z 68 and 123 was plotted against BA concentration ranged from as low as 0.44 μM after logarithmic transformation of both parameters. A linear regression line was yielded, which indicates BA concentration in ASM is directly proportional to the peak intensity, with R 2 =0.9052, significant at the 99% level. These results suggest that PyMS enables the quantitative determination of growth regulators and other related compounds in plant materials in a rapid, simple, sensitive, accurate manner.

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Genetic Discrimination of Catharanthus roseus Cultivars by Pyrolysis Mass Spectrometry

Abstract: Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex materials in a vacuum.

Cited by 7 publications

References 11 publications

Thermogravimetric analysis and Py-GC/MS for discrimination of leather from different animal species and tanning processes

Thermogravimetric analysis and Py-GC/MS for discrimination of leather from different animal species and tanning processes

The Complete Plastid Genome Sequence of Madagascar Periwinkle Catharanthus roseus (L.) G. Don: Plastid Genome Evolution, Molecular Marker Identification, and Phylogenetic Implications in Asterids

A Rapid, Simple Method for the Determination of 6-Benzyladenine by Pyrolysis Mass Spectrometry

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