Genetic differentiation of trout (Salmo SPP) populations in Serbia ascertained using RFLP technique on PCR amplified control region of mitochondrial DNA

Abstract

Genetic variability of trout populations from 13 localities situated in all three sea basins (Black, Aegean and Adriatic) of Serbia was ascertained using the restriction endonuclease Alu I, i.e., by RFLP (Restriction Fagment Length Polymorphism) technique on PCR (Polymerase Chain Reaction) amplified control region of the mtDNA. Restriction endonuclease Alu I cut the control region of the mtDNA at two characteristic profiles featured by populations of trout from the the Black Sea basin and those from basins of Aegean and Adriatic Seas ("southern" populations), respectively. This revealed a strong correlation between the geographic situation and the genetic differentiation of trout populations

Risks to stocks of native trout of the genus Salmo (Actinopterygii: Salmoniformes: Salmonidae) of Serbia and management for their recovery

“…Macedonian trout of the domesticated Adcs1 haplotype was stocked both into the Lisina Reservoir and into the lower section of the Jerma River (Marić et al 2006a) for fi shery purposes. It spread upstream into headwaters and easily naturalized there.…”

“…Species native to and introduced from streams of the Balkans area (e.g., of Da1, Da2, and Da22 haplotypes of brown trout; Adcs11 and Ad-Prz of Adriatic trout; Adcs1Macedonian trout; and Ohrid trout of unknown haplotype) were regarded as translocated. The proportion of trout holding alien haplotypes for each stream (Table 1) was calculated from records of genotyping (Marić et al 2006a, Tošić et al 2014 and from records for the Jerma River as provided in the presently reported study. Eleven trout individuals from the Jerma River sampled in 2013 by fl y fi shing were additionally analysed for their mtDNA haplotype using the methodology for DNA extraction and D-loop amplifi cation described in Tošić et al (2014).…”

“…Also, Macedonian trout was reportedly stocked into the Jerma River about 15 years ago (Marić et al 2006a), Lake Ohrid trout, Salmo letnica (Karaman, 1924), was heavily and repeatedly stocked from a hatchery into the Vlasina Lake in Southern Serbia in 1950s and 1960s for commercial fi shing purposes (Janković and Raspopović 1960).…”

“…Brown trout in streams of Serbia were found to belong to three of those lineages: Da, Ad, and At, each having several variants (haplotypes). In addition to the widespread native (Da1, Adcs1, and Adcs11) and introduced (Da2, Atcs1) mtDNA haplotypes, several new, native haplotypes were discovered in all three drainage areas of Southern Serbia (Marić et al 2006a, Tošić et al 2014. Some of them (Da-Vr, Da-Dž, and Ad-Bož) were found to be intermediate between the Danubian (Da) and Adriatic (Ad) lineages (after Bernatchez et al 1992).…”

“…Some of them (Da-Vr, Da-Dž, and Ad-Bož) were found to be intermediate between the Danubian (Da) and Adriatic (Ad) lineages (after Bernatchez et al 1992). Also, new and/or drainage-specifi c, native haplotypes of more advanced origin (Da-Vl, Ad-Ti, Ad-Prz, and Ad-Pe) of wild brown trout were recorded in south-western Serbia (Vlasina River), southern Kosovo (Tisova and Erenik rivers), and in the Metochia (Pećka and Prizrenska Bistrica rivers) (Marić et al 2006a). Likewise, the tentatively vicariant Da-s6 (Genbank accession code U18202) and Da22 haplotypes, which were so far known only from the Caspian Sea drainage area of Iran (Bernatchez 2001) and upper Danube River basin in Austria (Duftner et al 2003), respectively, were reported for the Studenačka and Rosomačka rivers in south-eastern Serbia.…”