Genetic differentiation of Allium sibiricum L. populations in Poland based on their morphological and molecular markers

Abstract

<em>Allium sibiricum</em> is one of the rarest plant species in the Sudetes and Carpathians. Inter simple sequence repeat DNA (ISSR) and morphological analyses were conducted to study the biogeographical relationships between geographically disjunctive populations of <em>A. sibiricum</em> in the Carpathians and Sudetes. The results clearly differentiated the Carpathian and Sudetes populations, but also showed a relatively high level of genetic similarity in specimens within certain groups of the Sudetes and Carpathian populations. The plants collected in the Karkonosze probably belong to a mountain form of <em>A. schoenoprasum</em> morphotype C which inhabits natural sites there. In contrast, the study found morphologically and genetically different plants inhabiting Pilsko Mt in the Carpathians. The plants from the Carpathians were present in scattered sites probably due to the activity of Vlach shepherds and the formation of large pastures. The species enlarged its local range due to this type of anthropopressure and likely hybridized with the cultivated <em>Allium</em> plants introduced by the shepherds. This may indicate that the populations on Pilsko Mt are of a partly anthropogenic character.

Intraspecific differentiation of Allium canadense var. canadense (Amaryllidaceae) across the North American Coastal Plain

“…Similar findings of high levels of genetic differentiation among natural populations were noted in a study of Allium munzii (Ownbey & Aase ex Traub) McNeal, a diploid sexually reproductive narrow endemic located in California, USA, in which 87.65% of variation occurred among populations compared to within populations, but high levels of haplotype and nucleotide diversity were only observed in two of the 11 populations studied (Mashayekhi & Columbus, 2015). In a study of Allium sibricum L., a diploid species that exhibits both sexual and vegetative reproduction, genetic differentiation was high among populations versus within populations as well, and genetic variation was high among plants from different regions (Urbaniak et al, 2019). However, studies of Allium mongolicum Regel, Allium altaicum Pall., and Allium cepa L. all showed greater variation within populations than among populations (Hu et al, 2022; Khapilina et al, 2021; Lyngkhoi et al, 2021).…”

Genetic diversity of Salix lapponum populations in Central Europe

“…PCR reactions were performed in 15-μl reaction tubes that contained a Dream Taq reaction buffer containing MgCl 2 , a 0.2 mM dNTP mix, 1u DreamTaq DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA), 0.5 mM ISSR primer, and 0.8 μl of total genomic DNA. The PCR cycle consisted of an initial denaturation at 95 °C for 6 min, according to a previous study (Urbaniak et al 2019), followed by 33 cycles at 95 °C for 30 seconds. The adequate annealing…”

Species diversity and phenetic relationship among accessions of api-api (Avicennia spp.) in Java based on morphological characters and ISSR markers

“…The use of cluster analysis employing the Euclidean distance matrix aims to group observational data into several groups with classification criteria based on the dissimilarity level. The closer to the 0 Euclidean distances, the higher the similarity (Urbaniak et al 2019). The results of the morphological cluster analysis of Avicennia spp.…”