Genetic Differentiation between European and North American Populations of Phlebiopsis gigantea

Vainio, Eeva J.; Hantula, Jarkko

doi:10.2307/3761502

Cited by 29 publications

(22 citation statements)

References 35 publications

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“…The sequence composition based separation technique of PCR products of the same length utilising chemical denaturants (urea and formamide) has become a standard method for rapid and accurate screening of complex microbial communities in clinical microbiology, food safety, and environmental microbiology (Muyzer et al 1993). Specific primers have been designed for assessing microbial communities, functional groups, genus, or species of complex ecosystems (Muyzer et al 1993;Nakatsu et al 2000;Vainio and Hantula 2000;Kowalchuk et al 2006;Mühling et al 2008;Cheng et al 2009). The strength of the DGGE method applied to soil ecology is the rapid, simple, reproducible, efficient (contemporary processing of a large number of samples), and low-cost way to assess microbial populations in complex communities and to monitor their response to environmental changes (Agnelli et al 2004;Lynch et al 2004;Ascher et al 2009a).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the denaturing gradient gel electrophoresis-apparatus as a parameter influencing soil microbial community fingerprinting

Ascher

Ceccherini

Chroňáková

et al. 2010

World J Microbiol Biotechnol

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We compared two denaturing gradient gel electrophoresis (DGGE) systems-DCode (Biorad, Hercules, CA, USA) and PhorU (Ingeny, Leiden, NL), performing community level 16S and 18S rRNA gene fragment-PCR-DGGE with total DNA extracted from upland pasture soil used for outdoor cattle husbandry. The methodological evaluation of the DGGE apparatus as parameter influencing DGGE fingerprinting, based on cluster analysis of soil bacterial and fungal community fingerprints, was made in terms of the resulting information about microbial community structures and their response to different degrees of cattle impact. Although the comparative DGGE analysis with different DGGE systems provided similar clustering of microbial community structures in correlation with the degree of cattle impact, our results suggest the DGGE system to be a factor influencing DGGE analysis. To our knowledge this is the first attempt to investigate the hypothetical impact of the DGGE system due to different technical characteristics, recommending the use of one and the same DGGE apparatus throughout an experiment, if the monitoring of microbial community structures requires multiple gel-to-gel analysis.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the denaturing gradient gel electrophoresis-apparatus as a parameter influencing soil microbial community fingerprinting

Ascher

Ceccherini

Chroňáková

et al. 2010

World J Microbiol Biotechnol

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“…Past research indicates that European isolates of P. gigantea should not be transferred to North America due to differences in their genetic compositions (Vainio and Hantula 2000). However, new data tend to show that the European and Canadian strains of P. gigantea can be regarded as representing the same biological species (Grillo et al 2005), but this is not an issue since we have efficacious isolates of P. gigantea from Canada (Bussières et al 1996).…”

Section: Découvrir La Revuementioning

confidence: 80%

Efficacy of two Phlebiopsis gigantea formulations in preventing Heterobasidion irregulare colonization of red pine stumps in eastern Canada

Dumas

Laflamme

2013

phyto

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Heterobasidion irregulare is the scientific name for the North American fungal species that was previously known as H. annosum (P-type) and Fomes annosus. In eastern Canada, the pathogen is found mainly in red pine plantations in southern Ontario and Quebec, where it causes tree mortality. There is no registered control method currently available for this disease in Canada. Phlebiopsis gigantea is a saprophytic basidiomycete successfully used and registered as a biological control agent in several European countries. In order to register a control product in Canada, its efficacy must be demonstrated under field conditions. Trials were performed with two Canadian isolates of P. gigantea in four red pine plantations in Ontario. The mean diameters of treated stumps ranged from 29 to 35 cm. After 2 mo, all 238 stumps treated were free of disease, while 12% of the 120 untreated stumps were colonized by the pathogen. The two formulations without P. gigantea did not prevent the colonization of the stumps by either P. gigantea or H. irregulare. These results show that the two Canadian isolates of P. gigantea can prevent colonization of red pine stumps by H. irregulare and provide support for the registration of P. gigantea as a biocontrol agent in eastern Canada.

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“…Vainio and Hantula [30] recently suggested even that European isolates should not be introduced into North American forests to avoid migration novel genotypes. It seems to be a discussion argument because transfer based on "the best" strains provides genetic recombination, and so to some extent, determines the adaptation of the species to environmental changes.…”

Section: Discussionmentioning

confidence: 99%

“…Total DNA of each isolate was extracted from 100 mg homogenized mycelium using GenElute Plant Genomic DNA Miniprep Kit (Sigma-Aldrich). The PCR amplifications were carried out with the primer 5'-DHB(CGA) 5 3' [28] consistent with RAMS method and primers' structure as following: [27,30]. For each DNA sample, three independent reactions were run.…”

Section: Dna Extraction Amplification Polymerase Chain Reaction (Pcmentioning

confidence: 99%

“…Fungus strains were obtained from Rotstop and PG Suspension producers and represented P. gigantea mycelium growing on coniferous stumps in Scandinavia (Finland, Sweden) and Great Britain, respectively. It was assumed that the results of molecular analyses carried out with the use of random amplified microsatellite (RAMS) method [27][28][29][30][31] as well as comparisons of strain enzymatic, and wood decay activities would distinguish similarities between the strains tested and point towards the most vigorous ones, in view of treatment efficacy in practice. The results of some enzyme assays and Norway spruce wood decay assessments described below were extracted from the database described by Żółciak et al [17].…”

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confidence: 99%

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What Is Important in Selecting Phlebiopsis gigantea Strain for Commercial Use?

Sierota¹,

Sikora²,

Żółciak³

2015

JAST-B

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Phlebiopsis gigantea strains registered in the European Union as biocontrol agents against root rot in forests (four from Great Britain and two from Finland) were compared using Ward's method with reference to: (1) similarity of DNA-random amplified microsatellite (RAMS) ladders, (2) cellulase and peroxidase production and (3) acceleration of dry mass wood loss in Norway spruce. The activity of the enzymes was tested in the initial phase of wood decay (30 d after inoculation) and indicated as the most active isolates: VF10 and FC15 for cellulase and FC16 and VF10 for peroxidase production. The assessment of loss of wood six months after inoculation indicated isolate FC15 as the most active. P. gigantea isolates similar in terms of enzyme activity indicated different patterns of DNA microsatellite loci. At the same time, DNA-RAMS revealed similarities in isolates with different abilities to produce enzymes. However, some similarities and differences between isolates according to wood decay were found. No plain relationships between molecular characteristics and enzyme activity of the strains tested were observed. The results differentiated activity of tested isolates and suggested benefits of selecting P. gigantea strains for commercial use basing mainly on the assessment of wood loss activity.

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Genetic Differentiation between European and North American Populations of Phlebiopsis gigantea

Cited by 29 publications

References 35 publications

Evaluation of the denaturing gradient gel electrophoresis-apparatus as a parameter influencing soil microbial community fingerprinting

Evaluation of the denaturing gradient gel electrophoresis-apparatus as a parameter influencing soil microbial community fingerprinting

Efficacy of two Phlebiopsis gigantea formulations in preventing Heterobasidion irregulare colonization of red pine stumps in eastern Canada

What Is Important in Selecting Phlebiopsis gigantea Strain for Commercial Use?

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