Genetic differentiation and phenetic relatedness among seven species of the Macrozamia plurinervia complex (Zamiaceae)

Sharma, Ish; Jones, Davey L.; Forster, Paul I.

doi:10.1016/j.bse.2003.07.002

Cited by 25 publications

(25 citation statements)

References 37 publications

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“…For this reason, the expected heterozygosity exceeded the average count of heterozygote genotypes in five out of six accessions, with an even higher excess (t test, P<0.0) in the whole collection (H T = 0.064; Het = 0.039). Both the polymorphic index (P = 20.05) and the heterozygosity estimated in annatto (H T = 0.064) were lower than reported for other woody species: P = 27.60 and H T = 0.131 (Hamrick & Loveless, 1986); P = 46.1 (Agarwal et al, 2001); H T = 0.242 (Lee et al, 2002); P = 49.0 and H T = 0.22 (Guo et al, 2003); P = 36.58 and H T = 0.11 (Sharma et al, 2004).…”

Section: Resultsmentioning

confidence: 79%

Isozymic variability in a Brazilian collection of annatto (Bixa orellana L.)

Carvalho

Robinson

Alfenas

2005

Pesq. agropec. bras.

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-The objectives of this work were to optimize the isozyme electrophoresis technique for Bixa orellana, and use isozyme markers for a preliminary survey on the genetic variability in Brazilian annatto germplasm accessions. Collection consisted of seed samples from sixty open pollinated trees, representing two Northern and four Southern geographic provenances. The extraction, electrophoresis, and interpretation of annatto isozymes are described. Three out of the twenty-one identified isozyme loci were polymorphic in the collection. The percentage of polymorphic loci (P = 21.05) and the expected heterozygosity in annatto (H T = 0.064) were low, compared to other tropical woody species. A UPGMA phenogram, constructed with Nei's genetic distances, clearly separated the germplasm provenant from North and Central Brazil. Variability was significantly higher among the accessions from Maranhão. A sharp genetic differentiation was detected between accessions from Maranhão and Pará States, despite their geographical proximity. The distinctive isozyme polymorphism, observed in the accessions from Maranhão, together with reports on local morphological heterogeneity in annatto fruit shape, color, and pubescence, calls for more detailed genetic and taxonomic investigation.Index terms: germplasm, lipstick plant, genetic variability, allozyme, phenogram. Variabilidade isoenzimática em uma coleção brasileira de urucum (Bixa orellana L.)Resumo -Os objetivos deste trabalho foram: otimizar a técnica de eletroforese de isoenzimas para Bixa orellana, e usar marcadores isoenzimáticos para um estudo preliminar da variabilidade genética, presente em uma coleção de germoplasma de urucum. Foi estudada uma coleção de amostras de sementes oriundas de 60 indivíduos de polinização aberta, que representam duas procedências do Norte e quatro do Sul do Brasil. São descritas a extração, a eletroforese e a interpretação de isoenzimas de urucum. Três, dos vinte e um locos isoenzimáticos identificados, foram polimórficos na coleção examinada. A porcentagem de locos polimórficos (P = 21,05) e a heterozigosidade esperada em urucum (H T = 0,064) foram baixas, em comparação com outras espécies arbóreas tropicais. O dendrograma UPGMA, construído com base nas distâncias genéticas de Nei, diferenciou claramente as procedências de urucum do Norte, daquelas oriundas do Brasil Central. A variabilidade foi significativamente superior na procedência Maranhão. Uma pequena diferenciação genética foi detectada entre os acessos do Maranhão e do Pará, apesar da proximidade geográfica. O polimorfismo isoenzimático diferenciado, observado no germoplasma procedente do Maranhão, somado a relatos de heterogeneidade morfológica local com relação à forma, à cor e à pubescência dos frutos de urucum, sugerem uma investigação genética e taxonômica mais detalhada dessa espécie.Termos para indexação: germoplasma, variabilidade genética, urucum, aloenzima, dendrograma.

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Section: Resultsmentioning

confidence: 79%

Isozymic variability in a Brazilian collection of annatto (Bixa orellana L.)

Carvalho

Robinson

Alfenas

2005

Pesq. agropec. bras.

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“…The genetic differentiation between species for the Ceratozamia norstogii complex (Fst= 0.04) was lower than the one reported for most of the Macrozamia complexes, for example M. pauli-guilelmi complex (0.47) (Sharma et al, 1998), M. plurinervia complex (0.588) (Sharma et al, 2004) and in general, for genus Ceratozamia (0.757) (De Castro, Vázquez-Torres, & De Luca, 2006) or cycads (0.2) (Pinares et al, 2009). However, its value was similar to the one reported for several species of Mexican cycads, such as Zamia purpurea (0.025), Z. variegata (0.085) (Pinares et al, 2009), Dioon merolae (0.07), D. caputoi (0.06) (Pinares et al, 2009), D. edule (González-Astorga et al, 2003) and to gymnosperms in general (0.068) (Hamrick & Godt, 1989).…”

Section: Discussionmentioning

confidence: 71%

“…The diversity within species (Hs) for the Ceratozamia norstogii complex (0.22) was higher than the one reported in most of Macrozamia complexes, for example M. heteromera (0.149), M. plurinervia (0.145) and M. pauli-guilelmi complex (0.12), but the diversity among species (Hb) (0.011) was lower than that for M. communis (0.1), M. plurinervia complex (0.207) (Sharma et al, 2004) and M. pauli-guilelmi complex (0.109) (Sharma et al, 1998); nevertheless, it was similar to the M. heteromera group (0.016) reports (Sharma et al, 1999).…”

Section: Discussionmentioning

confidence: 79%

“…The total genetic diversity (Ht) for the complex (0.23) was lower than that found for the Macrozamia plurinervia complex (0.352; Sharma et al, 2004); Encephalartos barteri (0.297; Ekué, Gailing, Hölscher, Sinsin, & Finkeldey, 2008) and Cycas simplicipinna (0.878; Feng, Wang, & Gong, 2014), but higher than Macrozamia communis (0.19;Ellstrand, Ornduff, & Clegg, 1990);M. heteromera (0.165;Sharma et al, 1999) and Cycas guizhouensis (0.108; Long-Qian, Xue-Jun, Xun, Gang, & SiXiang, 2004), and similar to the Macrozamia pauli-guilelmi complex (0.229; Sharma, Jones, Forster, & Young, 1998).…”

Section: Discussionmentioning

confidence: 95%

“…Isoenzymes may have different allelic forms (allozymes), mostly selective neutrals and co-dominants, which could be used to quantify allelic frequencies and different measures of genetic variation. Some analyses have been done on a wide range of cycad species, for instance; Zamia pumila (Walters & Deckers-Walters, 1991), Macrozamia pauli-guilielmi complex (Sharma, Jones, Forster, & Young, 1998), the M. heteromera complex (Sharma, Jones, Forster, & Young, 1999), the M. plurinervia complex (Sharma, Jones, & Forster, 2004), nine Encephalartos species (Van Der Bank et al, 2001), Cycas seemannii (Keppel, Lee, & Hodgskiss, 2002) and five species of Dioon have been analyzed (González-Astorga, Vovides, Ferrer, & Iglesias, 2003;González-Astorga, Vovides, Cruz-Angón, Octavio-Aguilar, & Iglesias 2005; Cabrera-Toledo, González-Astorga et al, 2008;Cabrera-Toledo et al, 2010). Genetic variation and species relationships have been studied to a lesser extent with RAPD markers, which are dominants and highly polymorphic, examples are Encephalartos (Chaiprasongsuk et al, 2004), with AFLP, RFLP and RAPDs in Cycas (Mekanawakul & Juntawong, 2004;Sangin, Thongpan, Lindstrom, Sangduan, & Mingmuang, 2004).…”

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confidence: 99%

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Variación genética en especies de cícadas estrechamente relacionadas en Ceratozamia (Zamiaceae: Cycadales) mediante el uso de RAPDs

Pérez‐Farrera

Vovides

González

et al. 2016

RBT

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The Ceratozamia norstogii complex from Southern Mexico is made up of four closely related taxa and occurs in similar habitats (Quercus forest). All have linear-lanceolate leaflets with great similarity between them, especially in juvenile stages, but differentiate with age. There has been debate regarding delimitation of species due to character loss in herbarium specimens. The aim of this study was to determine the genetic variation, and to measure genetic similarity between the four taxa. We studied populations in Cintalapa (Chiapas) for C. alvarezii and C. norstogii; the Sierra Atravesada (Oaxaca) for C. chimalapensis, and Villa Flores (Chiapas) for C. mirandae. One population for each taxon was sampled (only one population is known for C. alvarezii) 11-15 randomly chosen adult individuals were sampled. Twenty-eight primers were tested of which five were polymorphic using the RAPD’S technique. The data were analyzed using Bayesian methods. Results revealed low genetic diversity, and a differentiation was found between species, suggesting a recent divergence. A previous morphological and anatomical study on the complex has found the taxa to be distinct. However, the results of this study have shown that the C. norstogii species complex is in a divergence process, probably through genetic drift and founder effects.

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Relationships and Hybridization among Smilax china and Its Affinities: Evidence from Allozyme Data

Wang

Chen

et al. 2008

Biochem Genet

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Smilax china L. is a widespread species in China with different ploidy levels. It is morphologically similar to S. davidiana, S. trinervula, and S. glauco-china. In this study, the chromosome number and the variation in allozyme patterns of eight enzyme systems with 25 alleles in 11 populations of S. china and three affinitive species were investigated. The allozyme data, together with morphological and cytological data, suggest that S. glauco-china is not closely related to the other taxa investigated. The diploid species S. davidiana and S. trinervula are involved as ancestor species and share great introgressions with S. china. In S. china, populations from Guilin and Guiyang are allotetraploid; their diploid progenitors probably are diploid populations of S. china and S. trinervula. The results suggest this species arose from multiple origins.

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Genetic differentiation and phenetic relatedness among seven species of the Macrozamia plurinervia complex (Zamiaceae)

Cited by 25 publications

References 37 publications

Isozymic variability in a Brazilian collection of annatto (Bixa orellana L.)

Isozymic variability in a Brazilian collection of annatto (Bixa orellana L.)

Variación genética en especies de cícadas estrechamente relacionadas en Ceratozamia (Zamiaceae: Cycadales) mediante el uso de RAPDs

Relationships and Hybridization among Smilax china and Its Affinities: Evidence from Allozyme Data

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