Genetic differentiation among natural populations of Gastrodia elata (Orchidaceae) in Hubei and germplasm assessment of the cultivated populations

Wu, Huifang

doi:10.1360/biodiv.060053

Cited by 29 publications

(14 citation statements)

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“…The results from the Mantel test also supported the above observation as it showed no correlation between the genetic and geographic distances between different populations. The ultimate goal of conservation is to ensure the continuous survival of population and to maintain their evolutionary potential by preserving natural levels of genetic diversity within and between populations [9, 56, 57]. The natural M. baccifera resources in the selected sites have been exploited by locals for many years leading to loss of plant resources, habitat destruction, and fragmentation.…”

Section: Discussionmentioning

confidence: 99%

ISSR Marker Based Population Genetic Study ofMelocanna baccifera(Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India

et al. 2017

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Melocanna baccifera (Roxb.) Kurz is an economically important bamboo of North-East India experiencing population depletion in its natural habitats. Genetic variation studies were conducted in 7 populations sampled from 5 districts of Manipur using ISSR molecular markers. The investigation was carried out as a primary step towards developing effective conservation strategies for the protection of bamboo germplasm. ISSR marker analysis showed significant level of genetic variation within the populations as revealed by moderately high average values of Nei's genetic diversity (H 0.1639), Shannon's diversity index (I 0.2563), percentage of polymorphic bands (PPB 59.18), total genetic variation (Ht 0.1961), and genetic diversity within population (Hs 0.1639). The study also divulged a high genetic variation at species level with Shannon's diversity index (I), Nei's genetic diversity (H), and percentage of polymorphic band (PPB%) recorded at 0.3218, 0.1939, and 88.37, respectively. Genetic differentiation among the populations (Gst) was merely 19.42% leaving 80.58% of genetic variation exhibited within the populations. The low genetic diversity between populations was consistent with AMOVA. The low genetic differentiation among populations coupled with existence of significantly high genetic diversity at species level indicated the urgent necessity of preserving and protecting all the existing natural bamboo populations in the region.

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Section: Discussionmentioning

confidence: 99%

ISSR Marker Based Population Genetic Study ofMelocanna baccifera(Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India

et al. 2017

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“…Our findings were similar to several other long-lived cultivated plants in which genetic diversity is as high as in the wild relatives (He et al , 2007; Shi et al , 2008). Cultivated R. tanguticum had moderate levels of genetic diversity compared with cultivated populations of other endangered Chinese medicinal plants (Table 6) (Wu et al , 2006; Guo et al , 2007; Qiu et al , 2009; Song et al , 2010; Li et al , 2011; Yao et al , 2012), i.e. , there was no evidence that a “cultivation bottleneck or founder effect” affected the genetic diversity.…”

Section: Discussionmentioning

confidence: 99%

Genetic variation in cultivated Rheum tanguticum populations

et al. 2014

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To examine whether cultivation reduced genetic variation in the important Chinese medicinal plant Rheum tanguticum, the levels and distribution of genetic variation were investigated using ISSR markers. Fifty-eight R. tanguticum individuals from five cultivated populations were studied. Thirteen primers were used and a total of 320 DNA bands were scored. High levels of genetic diversity were detected in cultivated R. tanguticum (PPB = 82.19, H = 0.2498, HB = 0.3231, I = 0.3812) and could be explained by the outcrossing system, as well as long-lived and human-mediated seed exchanges. Analysis of molecular variance (AMOVA) showed that more genetic variation was found within populations (76.1%) than among them (23.9%). This was supported by the coefficient of gene differentiation (Gst = 0.2742) and Bayesian analysis (θB = 0.1963). The Mantel test revealed no significant correlation between genetic and geographic distances among populations (r = 0.1176, p = 0.3686). UPGMA showed that the five cultivated populations were separated into three clusters, which was in good accordance with the results provided by the Bayesian software STRUCTURE (K = 3). A short domestication history and no artificial selection may be an effective way of maintaining and conserving the gene pools of wild R. tanguticum.

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“…Although several studies have examined the effects of cultivation on the genetic structure of crop species and fruit tree populations in China (Cai 2006; Zhu et al. 2007), little is known about how the cultivation process has impacted the extent and distribution of genetic variation in populations of the Chinese medicinal plant species (Wu et al. 2006).…”

Section: Introductionmentioning

confidence: 99%

“…2006) and levels of genetic variation among wild and cultivated populations (Qiu et al. 2003; Wu et al. 2006).…”

Section: Introductionmentioning

confidence: 99%

Genetic variation among wild and cultivated populations of the Chinese medicinal plantCoptis chinensis(Ranunculaceae)

et al. 2008

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To examine if the cultivation process has reduced the genetic variation of modern cultivars of the traditional Chinese medicinal plant, Coptis chinensis, the levels and distribution of genetic variation was investigated using ISSR markers. A total of 214 C. chinensis individuals from seven wild and three cultivated populations were included in the study. Seven ISSR primers were used and a total of 91 DNA fragments were scored. The levels of genetic diversity in cultivated populations were similar as those in wild populations (mean PPL = 65.2% versus PPL = 52.4%, mean H = 0.159 versus H = 0.153 and mean I = 0.255 versus I = 0.237), suggesting that cultivation did not seriously influence genetic variation of present-day cultivated populations. Neighbour-joining cluster analysis showed that wild populations and cultivated populations were not separated into two groups. The coefficient of genetic differentiation between a cultivar and its wild progenitor was 0.066 (G(st)), which was in good accordance with the result by amova analysis (10.9% of total genetic variation resided on the two groups), indicating that cultivated populations were not genetically differentiated from wild progenitors. For the seven wild populations, a significant genetic differentiation among populations was found using amova analysis (45.9% of total genetic variation resided among populations). A number of causes, including genetic drift and inbreeding in the small and isolated wild populations, the relative limited gene flow between wild populations (N(m) = 0.590), and high gene flow between cultivars and their wild progenitors (N(m) = 7.116), might have led to the observed genetic profiles of C. chinensis.

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Genetic differentiation among natural populations of Gastrodia elata (Orchidaceae) in Hubei and germplasm assessment of the cultivated populations

Cited by 29 publications

References 0 publications

ISSR Marker Based Population Genetic Study ofMelocanna baccifera(Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India

ISSR Marker Based Population Genetic Study ofMelocanna baccifera(Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India

Genetic variation in cultivated Rheum tanguticum populations

Genetic variation among wild and cultivated populations of the Chinese medicinal plantCoptis chinensis(Ranunculaceae)

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