Genetic differentiation among geographic populations of Gonatocerus ashmeadi, the predominant egg parasitoid of the glassy-winged sharpshooter, Homalodisca coagulata

León, Jesse H. de; Jones, Walker A.

doi:10.1673/031.005.0201

Cited by 13 publications

(7 citation statements)

References 46 publications

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“…Other genetic studies on parasitoid Hymenoptera have considered the level of the population rather than the level of individual patches, and have produced varying results on the population substructuring and the level of inbreeding. de Leon & Jones (2005) found for Gonatocerus ashmeadi a pronounced genetic structure between samples from the American East-and West Coast (G ST = 0.38), while Kankare et al (2005) found differing results for Cotesia melitaearum and Hyposoter horticola. F ST for C. melitaearum was much higher than for H. horticola (0.378 vs. 0.063), and both species showed significant isolation by distance.…”

Section: Introductionmentioning

confidence: 94%

Genetic structure of natural Nasonia vitripennis populations: validating assumptions of sex‐ratio theory

et al. 2008

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The parasitic wasp Nasonia vitripennis has been used extensively in sex allocation research. Although laboratory experiments have largely confirmed predictions of local mate competition (LMC) theory, the underlying assumptions of LMC models have hardly been explored in nature. We genotyped over 3500 individuals from two distant locations (in the Netherlands and Germany) at four polymorphic microsatellite loci to validate key assumptions of LMC theory, in terms of both the original models and more recent extensions to them. We estimated the number of females contributing eggs to patches of hosts and the clutch sizes as well as sex ratios produced by individual foundresses. In addition, we evaluated the level of inbreeding and population differentiation. Foundress numbers ranged from 1 to 7 (average 3.0 ± 0.46 SE). Foundresses were randomly distributed across the patches and across hosts within patches, with few parasitizing more than one patch. Of the hosts, 40% were parasitized by more than one foundress. Clutch sizes of individual foundresses (average 9.99 ± 0.51 SE) varied considerably between hosts. The time period during which offspring continued to emerge from a patch or host correlated strongly with foundress number, indicating that sequential rather than simultaneous parasitism is the more common. Genetic differentiation at the regional level between Germany and the Netherlands, as estimated by Slatkin's private allele method (0.11) and Hedrick's corrected G′ LT (0.23), indicates significant substructuring between regions. The level of population inbreeding for the two localities (F IL = 0.168) fitted the expectation based on the average foundress number per patch.

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Section: Introductionmentioning

confidence: 94%

Genetic structure of natural Nasonia vitripennis populations: validating assumptions of sex‐ratio theory

et al. 2008

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“…Total genomic DNA was extracted according to standard methods (Sambrook & Russell, 2001) as previously described (de Leó n et al, 2004b(de Leó n et al, , 2006de Leó n & Jones, 2005). Briefly, individual wasps were homogenized on ice in 1.5 ml microfuge tubes in 60 ml of lysis buffer [10 mM Tris-HCl (pH 7.5), 1 mM EDTA (pH 7.5), 1% IGEPAL CA-630)] with two 20-s bursts with 10 min intervals on ice (Pellet Pestle Motor, Bel-Art Products, Pequannock, NJ).…”

Section: Genomic Dna Isolationmentioning

confidence: 99%

“…ISSR-PCR is a sensitive technique because it targets random simple sequence repeats or microsatellites within the entire genome, thus revealing highly polymorphic banding patterns or multiple loci (Zietkiewicz et al , 1994). Therefore, increased power to resolve genetic relationships comes with information from many loci within the nuclear DNA (Zietkiewicz et al , 1994; Karp & Edwards, 1997; Wolfe & Liston, 1998; de León & Jones, 2004, 2005; de León et al ., 2004a,b, 2006). Similarly, phylogenetics utilizing mitochondrial DNA, typically inherited as a single locus, is a widespread approach for delineating and identifying morphologically similar or cryptic species and identifying the geographic origins of invasive species (Rosen, 1977; Narang et al , 1993; Schauff & LaSalle, 1998; Unruh & Woolley, 1999; Avise, 2000; Roderick & Navajas, 2003; Brown, 2004; MacDonald & Loxdale, 2004; Roderick, 2004).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization ofGonatocerus tuberculifemur(Ogloblin) (Hymenoptera: Mymaridae), a prospectiveHomalodisca vitripennis(Germar) (Hemiptera: Cicadellidae) biological control candidate agent from South America: divergent clades

León

Logarzo

Triapitsyn

2007

Bull. Entomol. Res.

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We genetically characterized the prospective South American egg parasitoid candidate, Gonatocerus tuberculifemur, of the glassy-winged sharpshooter (GWSS), Homalodisca vitripennis, for a neoclassical biological control program in California. Two molecular methods, inter-simple sequence repeat-polymerase chain reaction DNA fingerprinting and a phylogeographic approach inferred from the mitochondrial cytochrome oxidase subunit I gene (COI), were utilized. Five geographic populations from South America were analyzed; in addition, a phylogenetic analysis was performed with several named and one unnamed Gonatocerus species using the COI gene. DNA fingerprinting demonstrated a fixed geographic banding pattern difference in the population from San Rafael, Mendoza Province, Argentina. The COI analysis uncovered haplotype or geographic structure in G. tuberculifemur. A neighbour-joining distance (NJ) and a single most parsimonious tree (MP) clustered the populations into two well-supported distinct clades with strong bootstrap values (97-99% and 92-99%, respectively) with populations from San Rafael clustering into clade 2 and the rest of the populations clustering into clade 1. No haplotype sharing was observed between individuals from the two clades. Phylogenetic analyses performed by NJ and MP methods with 15 Gonatocerus species confirmed species boundaries and again uncovered two distinct clades in G. tuberculifemur with strong bootstrap support (95-100% and 68-100%, respectively). However, the NJ tree supported the morphologically defined relationships better than the MP tree. The molecular evidence in the present study is suggestive of a species level divergence. Because G. tuberculifemur is under consideration as a potential biological control agent for GWSS in California, understanding cryptic variation in this species is critical.

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“…ISSR-based techniques enable the development of co-dominant microsatellite markers that may be used in population studies (Gupta et al, 1994;Bornet & Branchard, 2001;Wolfe, 2005). These techniques have allowed genetic distinctions to be drawn among Hymenoptera groups (Borba et al, 2005;León & Jones, 2005;Al-Otaibi, 2008) and among other Insecta groups (Souza et al, 2008;Luque et al, 2002;Dusinsky et al, 2006). However, at present, no known published studies in the literature have applied these techniques exclusively for the examination of ants.…”

mentioning

confidence: 99%

The genetic characterization of Myrmelachista spp. Roger (Hymenoptera: Formicidae) specimens collected in the Atlantic Forest of southeastern Brazil

Nakano¹,

Miranda²,

Feistosa³

et al. 2014

Sociobiology

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Arboreal ants of the genus Myrmelachista, which have ecologically important relationships with different vegetable species, are found exclusively in the Neotropical region. These ant species are difficult to identify, and their taxonomy remains controversial; moreover, little is known regarding their biology. The objective of the present work is to assess the genetic similarities and dissimilarities between and within Myrmelachista species, with the goal of expanding knowledge of the relationships among the taxa of this genus. Sample collection in selected regions of the dense ombrophile forest of southeastern Brazil yielded 256 nests, which were found in vegetation or among scattered twigs in the leaf litter; eight species were recorded. A total of 180 specimens were analyzed, producing 123 molecular characters. Data analyses revealed similarity relationships that allowed the examined species to be classified into the following groups: (1) Myrmelachista sp. 4, M. nodigera, M. ruszkii and M. gallicola; (2) M. catharinae and M. arthuri; (3) M. reticulata; and (4) Myrmelachista sp. 7. The study results also revealed the existence of two morphological variants of M. catharinae; M. arthuri was more closely related to one of these M. catharinae variants than to the other variant. The present work provides important information regarding genetic variation among Myrmelachista species that may contribute to interpreting the complex morphology of this genus.

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Genetic differentiation among geographic populations of Gonatocerus ashmeadi, the predominant egg parasitoid of the glassy-winged sharpshooter, Homalodisca coagulata

Cited by 13 publications

References 46 publications

Genetic structure of natural Nasonia vitripennis populations: validating assumptions of sex‐ratio theory

Genetic structure of natural Nasonia vitripennis populations: validating assumptions of sex‐ratio theory

Molecular characterization ofGonatocerus tuberculifemur(Ogloblin) (Hymenoptera: Mymaridae), a prospectiveHomalodisca vitripennis(Germar) (Hemiptera: Cicadellidae) biological control candidate agent from South America: divergent clades

The genetic characterization of Myrmelachista spp. Roger (Hymenoptera: Formicidae) specimens collected in the Atlantic Forest of southeastern Brazil

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