Genetic Differences of Three Pollicipes mitella Populations Identified by PCR Analysis

Abstract

Genomic DNAs were extracted from the turtle leg (Pollicipes mitella, 1798) population of Tongyeong, Yeosu and Manjaedo located in the southern sea of Korea. The turtle leg population from Tongyeong (0.929) exhibited higher bandsharing values than did turtle leg from Manjaedo (0.852). The higher fragment sizes (>1,200 bp) are much more observed in the Yeosu population. The number of unique loci to each population and number of shared loci by the three populations, generated by PCR using 7 primers in the turtle leg (P. mitella) population of Tongyeong, Yeosu and Manjaedo. Genetic distances among different individuals of the Tongyeong population of the turtle leg (lane 1-07), Yeosu population of the turtle leg (lane 08-14) and Manjaedo population of the turtle leg (lane 15-21), respectively, were generated using the CLASSIFICATION option in Systat version 10 according to the bandsharing values and similarity matrix. The dendrogram, obtained by the seven decamer primers, indicated three genetic clusters: cluster 1 (TONGYEONG 01-TONGYEONG 07), cluster 2 (YEOSU 08-YEOSU 14), and cluster 3 (MANJEDO 15-MANJEDO 21). Tongyeong population could be evidently discriminated with the other two Yeosu and Manjaedo populations among three populations. The longest genetic distance (0.305) was found to exist between individuals’ no. 02 of the Tongyeong population and no. 13 of the Yeosu population. It seems to the authors that this is a result of a high degree of inbreeding in narrow region for a long while.

Genetic Distances of Three Mollusk Species Investigated by PCR Analysis

“…PCR analysis was carried out on DNA samples extracted from a total of 21 individuals using seven decamer oligonucleotides primers. DNA extraction should be carried out according to the separation and extraction methods ( Song & Yoon, 2013 ). After several washings, lysis buffer I (155 mM NH 4 Cl; 10 mM KHCO 3 ; 1 mM EDTA) was added to the samples, and the mixture tubes were gently inverted.…”

Genetic Distances and Variations of Three Clupeid Species Determined by PCR Technique

“…Many researchers studied the sizes of DNA fragments in the PCR profiles of barramundi (Lates calcarifer) (Partis & Wells, 1996), four species of the Mullidae family (Mamuris et al, 1999), the eastern Pacific yellowfin tuna (Thunnus albacares) (Diaz-Jaimes & Uribe-Alcocer, 2003), five species of Korean catfish (Silurus asotus) (Yoon & Kim, 2004), venus clam (Gomphina aequilatera) (Kim et al, 2006) and the turtle leg (Pollicipes mitella) (Song & Yoon, 2013).…”

“…Three species of fish muscle was collected in sterile tubes, placed on ice immediately, and stored under refrigeration until needed. Genomic DNA was extracted and purified under the conditions described previously (Yoon & Kim, 2004;Song & Yoon, 2013). The DNA pellets were incubation-dried for 2 hrs, held at -40°C until analysis, and then dissolved in the TE buffer (10 mM Tris- …”

Genetic Distances between Two Echiuran Populations Discriminated by PCR

“…Detailed information on genetic markers specific to lines, breeds, species, and/or geographical populations has been used for the identification and discrimination of individuals and populations, as well as hybrid pedigrees, and for molecular genetic testing (Mamuris et al, 1999;Klinbunga et al, 2000a;Nozaki et al, 2000;Yoon & Park, 2002;Kim et al, 2004;Park et al, 2005;Yoon & Kim, 2010;Yoon et al, 2012;Song & Yoon, 2013;Oh & Yoon, 2014). Accordingly, adaptations of PCR methods to the mariculture and fishing industry have been used to estimate genetic distances (GDs) among some finfish species and/or invertebrate animals from different geographical locations (Callejas & Ochando, 1998;Tassanakajon et al, 1998;Klinbunga et al, 2000b;McCormack et al, 2000;Yoon & Park, 2002;Kim et al, 2004;Park et al, 2005;Song & Yoon, 2013;Oh & Yoon, 2014). In this study, to evaluate the GDs and dissimilarities between geographical echiuran populations, clustering analysis of two Urechis spp.…”

“…Several studies have reported the sizes of PCR fragments for black tiger shrimp (Penaeus monodon) (Tassanakajon et al, 1998), the brittle (McCormack et al, 2000), and lobster species (Park et al, 2005). Unambiguous data, resulting from variations in DNA polymorphisms, were found to be meaningful in the identification of individuals and/or populations (Tassanakajon et al, 1998;Mamuris et al, 1999;Yoon & Park, 2002;Chenyambuga et al, 2004;Kim et al, 2004;Yoon & Kim, 2010;Yoon et al, 2012;Song & Yoon, 2013).…”