Genetic differences in wild strains of Lentinula edodes collected from a single fallen tree

Abstract

Genetic differences among 18 Lentinula edodes strains isolated from a fallen trunk of Quercus mongolica var. grosseserrata were characterized by mating tests and restriction fragment length polymorphism (RFLP) analyses of mitochondrial DNA (mtDNA). These strains could be divided into six genets of different mating types. Because the mtDNA of the 18 strains showed four different RFLP genotypes, these strains seemed to have originated from at least 4 distinct parental strains. Strains belonging to the same genet were collected from fruiting bodies located not more than about 1 m apart on the fallen tree. Implications of these findings regarding the degree of genetic variation and territory sizes of individual genets of wood-decaying basidiomycetes such as L. edodes are discussed.

Genetic variability among <i>Pholiota aurivella</i> isolates from a small natural population

“…(Smith et al 1992). Basidiospore dispersal is probably the main means of spread of nonpathogenic wood-decaying basidiomycetes, and small single genet territories within a single fallen tree have been reported for P. ostreatus (Kay and Vilgalys 1992), L. edodes (Chiu et al 1999;Fukuda and Mori 2003), and F. velutipes (Fukuda et al 2000). From the results of these studies, we can estimate that the individual genets of these wood-decaying basidiomycetes do not extend much more than about 1 m in diameter.…”

“…Sing. (Nishizawa et al 2003), and genetic differences between wild strains growing on the same substrates have been studied within the context of the size of their inhabited territory in some wood-decaying basidiomycetes such as P. ostreatus (Kay and Vilgalys 1992), L. edodes (Chiu et al 1999;Fukuda and Mori 2003), and F. velutipes (Fukuda et al 2000). However, offspring derived from extensively cultivated strains may have increased in natural populations, which may be refl ected in the genetic variability of the natural populations.…”

Genetic variability among Pholiota aurivella isolates from a small natural populatio

“…(Smith et al 1992). Basidiospore dispersal is probably the main means of spread of nonpathogenic wood-decaying basidiomycetes, and small single genet territories within a single fallen tree have been reported for P. ostreatus (Kay and Vilgalys 1992), L. edodes (Chiu et al 1999;Fukuda and Mori 2003), and F. velutipes (Fukuda et al 2000). From the results of these studies, we can estimate that the individual genets of these wood-decaying basidiomycetes do not extend much more than about 1 m in diameter.…”

“…Sing. (Nishizawa et al 2003), and genetic differences between wild strains growing on the same substrates have been studied within the context of the size of their inhabited territory in some wood-decaying basidiomycetes such as P. ostreatus (Kay and Vilgalys 1992), L. edodes (Chiu et al 1999;Fukuda and Mori 2003), and F. velutipes (Fukuda et al 2000). However, offspring derived from extensively cultivated strains may have increased in natural populations, which may be refl ected in the genetic variability of the natural populations.…”