Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth

Schinner, Silvia; Engelhardt, Florian; Preuße, Matthias; Thöming, Janne G.; Tomasch, Jürgen; Häußler, Susanne

doi:10.1016/j.bioflm.2020.100023

Cited by 16 publications

(20 citation statements)

References 75 publications

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“…This may be observed in dental caries on the tooth surfaces [85,86], in skin and soft tissue infections [52], in infections of the middle ear [87], catheter-associated infections [19], pneumonia, and in the lungs of CF patients [88]. In the latter case, P. aeruginosa is able to survive and avoid clearance (withstanding the immune response and the subsequent administration of antimicrobials) in the respiratory and conductive zone of the lungs [89,90]. For example, alginate and other polysaccharides produced by the mucoid variants are effective in scavenging ROS, protecting bacterial cells [91].…”

Section: Biofilm Formationmentioning

confidence: 99%

“…It has been noted by several publications that the minimum inhibitory concentrations (MICs) of bacteria inside the biofilm may be 10-10,000 times higher, compared to planktonic cells [75][76][77][78][79]83,84,[88][89][90]. On one hand, the secreted extracellular matrix significantly hinders the diffusion of the antibiotic molecules to effectively reach the bacterial targets (pharmacokinetic barrier); in addition to this, bacteria residing in the deeper layers of the biofilm will adapt to a differentiated metabolic state [75][76][77][78][79]83,84,[88][89][90]. It must be noted that the inhibition of bacterial growth is mechanistically distinct from bacterial killing, and antimicrobials (even in effective doses) may not kill cells inside a biofilm.…”

Section: Biofilm Formationmentioning

confidence: 99%

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It’s Not Easy Being Green: A Narrative Review on the Microbiology, Virulence and Therapeutic Prospects of Multidrug-Resistant Pseudomonas aeruginosa

2021

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Pseudomonas aeruginosa is the most frequent cause of infection among non-fermenting Gram-negative bacteria, predominantly affecting immunocompromised patients, but its pathogenic role should not be disregarded in immunocompetent patients. These pathogens present a concerning therapeutic challenge to clinicians, both in community and in hospital settings, due to their increasing prevalence of resistance, and this may lead to prolonged therapy, sequelae, and excess mortality in the affected patient population. The resistance mechanisms of P. aeruginosa may be classified into intrinsic and acquired resistance mechanisms. These mechanisms lead to occurrence of resistant strains against important antibiotics—relevant in the treatment of P. aeruginosa infections—such as β-lactams, quinolones, aminoglycosides, and colistin. The occurrence of a specific resistotype of P. aeruginosa, namely the emergence of carbapenem-resistant but cephalosporin-susceptible (Car-R/Ceph-S) strains, has received substantial attention from clinical microbiologists and infection control specialists; nevertheless, the available literature on this topic is still scarce. The aim of this present review paper is to provide a concise summary on the adaptability, virulence, and antibiotic resistance of P. aeruginosa to a readership of basic scientists and clinicians.

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Section: Biofilm Formationmentioning

confidence: 99%

Section: Biofilm Formationmentioning

confidence: 99%

It’s Not Easy Being Green: A Narrative Review on the Microbiology, Virulence and Therapeutic Prospects of Multidrug-Resistant Pseudomonas aeruginosa

2021

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“…However, the links between increased stress survival and competitive advantage in culture have been explored in other situations, and the picture seems to be much more complex than initially perceived. Some studies support a strong link between the two (Bruggeman et al, 2020;Schinner et al, 2020;Schirrmacher, 2021). Other studies have failed to find a simple correlation and have attributed this to the specific system they were exploring (Bleibtreu et al, 2013).…”

Section: Review the Links Between Changes In Stress Survival And Changes In Competitive Fitnessmentioning

confidence: 99%

Endurance of extremely prolonged nutrient prevention across kingdoms of life

2021

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Numerous observations demonstrate that microorganisms can survive very long periods of nutrient deprivation and starvation. Moreover, it is evident that prolonged periods of starvation are a feature of many habitats, and many cells in all kingdoms of life are found in prolonged starvation conditions. Bacteria exhibit a range of responses to long-term starvation. These include genetic adaptations such as the long-term stationary phase and the growth advantage in stationary phase phenotypes characterized by mutations in stress-signaling genes and elevated mutation rates. Here, we suggest using the term "endurance of prolonged nutrient prevention" (EPNP phase), to describe this phase, which was also recently described in eukaryotes. Here, we review this literature and describe the current knowledge about the adaptations to very long-term starvation conditions in bacteria and eukaryotes, its conceptual and structural conservation across all kingdoms of life, and point out possible directions that merit further research.

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“…RNA extraction was carried out as described in the previous section. RNA libraries were prepared as described previously (Schinner et al, 2020 Sequencing reads were quality controlled, adapter clipped using fastq-mcf from the ea-utils package (Aronesty, 2013) and mapped to the reference genome UCBPP-PA14 (NC_008463.1)…”

Section: Rna Sequencingmentioning

confidence: 99%

Analysis of the organization and expression patterns of the convergent Pseudomonas aeruginosa lasR/rsaL gene pair uncovers mutual influence

Schinner

Preuße

Kesthely

et al. 2020

Molecular Microbiology

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Pseudomonas aeruginosa is a ubiquitous environmental bacterium, which has emerged as an opportunistic pathogen of major clinical relevance (Bassetti et al., 2018). Its extensive metabolic versatility facilitates survival not only in different aquatic and terrestrial habitats, but also in the human host. P. aeruginosa infections can become established at various host sites and progress into life threatening acute or chronic infections (Lorenz et al., 2019;Moradali et al., 2017;Turner et al., 2014). The pathogen is equipped with a large arsenal of virulence factors. Expression of many of the respective P. aeruginosa virulence genes is orchestrated by two hierarchical organized homoserine lactone (HSL)-dependent quorum-sensing (QS) systems, the las and the rhl system (

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Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth

Cited by 16 publications

References 75 publications

It’s Not Easy Being Green: A Narrative Review on the Microbiology, Virulence and Therapeutic Prospects of Multidrug-Resistant Pseudomonas aeruginosa

It’s Not Easy Being Green: A Narrative Review on the Microbiology, Virulence and Therapeutic Prospects of Multidrug-Resistant Pseudomonas aeruginosa

Endurance of extremely prolonged nutrient prevention across kingdoms of life

Analysis of the organization and expression patterns of the convergent Pseudomonas aeruginosa lasR/rsaL gene pair uncovers mutual influence

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