Genetic Determinants for Enzymatic Digestion of Lignocellulosic Biomass Are Independent of Those for Lignin Abundance in a Maize Recombinant Inbred Population

Penning, Bryan W.; Sykes, Robert; Babcock, Nicholas C.; Dugard, Christopher K.; Held, Michael; Klimek, John F.; Shreve, Jacob; Fowler, Matthew; Ziebell, Angela; Davis, Mark F.; Decker, Stephen R.; Turner, Geoffrey B.; Mosier, Nathan S.; Springer, Nathan M.; Thimmapuram, Jyothi; Weil, Clifford F.; McCann, Maureen C.; Carpita, Nicholas C.

doi:10.1104/pp.114.242446

Cited by 51 publications

(54 citation statements)

References 83 publications

(106 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides selecting or breeding for natural variation in biomass composition (Wegrzyn et al, 2010;Penning et al, 2014a), it is also possible to genetically modify biomass composition (Bartley and Ronald, 2009). Most simply, genetic engineering of bioenergy plants can be achieved by modifying the plant's genome to (1) express genes from other organisms, (2) increase expression of native genes, or (3) reduce expression of native genes.…”

Section: Resultsmentioning

confidence: 99%

Relationships between Biomass Composition and Liquid Products Formed via Pyrolysis

et al. 2015

View full text Add to dashboard Cite

Thermal conversion of biomass is a rapid, low-cost way to produce a dense liquid product, known as bio-oil, that can be refined to transportation fuels. However, utilization of bio-oil is challenging due to its chemical complexity, acidity, and instability -all results of the intricate nature of biomass. A clear understanding of how biomass properties impact yield and composition of thermal products will provide guidance to optimize both biomass and conditions for thermal conversion. To aid elucidation of these associations, we first describe biomass polymers, including phenolics, polysaccharides, acetyl groups, and inorganic ions, and the chemical interactions among them. We then discuss evidence for three roles (i.e., models) for biomass components in the formation of liquid pyrolysis products: (1) as direct sources, (2) as catalysts, and (3) as indirect factors whereby chemical interactions among components and/or cell wall structural features impact thermal conversion products. We highlight associations that might be utilized to optimize biomass content prior to pyrolysis, though a more detailed characterization is required to understand indirect effects. In combination with high-throughput biomass characterization techniques, this knowledge will enable identification of biomass particularly suited for biofuel production and can also guide genetic engineering of bioenergy crops to improve biomass features.

show abstract

Section: Resultsmentioning

confidence: 99%

Relationships between Biomass Composition and Liquid Products Formed via Pyrolysis

et al. 2015

View full text Add to dashboard Cite

show abstract

“…However, because many QTL analyses performed using linkage mapping (e.g. involving RIL or DH populations) result in large QTL confidence intervals (Messmer et al, 2009;Hao et al, 2010;Li et al, 2010;Almeida et al, 2013;Penning et al, 2014;Park et al, 2015) and the respective individual linkage maps may vary greatly (Semagn et al, 2013), relatively wide chromosomal segments have to be considered and such co-locations have thus to be regarded as tentative. Nevertheless, Peiffer et al (2014) showed that the strongest GWAS associations were often located near the most robust family-nested QTL.…”

Section: Only a Subset Of The Detected Loci Has Been Identified In Prmentioning

confidence: 99%

Genetic variation of growth dynamics in maize (Zea mays L.) revealed through automated non‐invasive phenotyping

et al. 2017

View full text Add to dashboard Cite

Hitherto, most quantitative trait loci of maize growth and biomass yield have been identified for a single time point, usually the final harvest stage. Through this approach cumulative effects are detected, without considering genetic factors causing phase-specific differences in growth rates. To assess the genetics of growth dynamics, we employed automated non-invasive phenotyping to monitor the plant sizes of 252 diverse maize inbred lines at 11 different developmental time points; 50 k SNP array genotype data were used for genome-wide association mapping and genomic selection. The heritability of biomass was estimated to be over 71%, and the average prediction accuracy amounted to 0.39. Using the individual time point data, 12 main effect marker-trait associations (MTAs) and six pairs of epistatic interactions were detected that displayed different patterns of expression at various developmental time points. A subset of them also showed significant effects on relative growth rates in different intervals. The detected MTAs jointly explained up to 12% of the total phenotypic variation, decreasing with developmental progression. Using non-parametric functional mapping and multivariate mapping approaches, four additional marker loci affecting growth dynamics were detected. Our results demonstrate that plant biomass accumulation is a complex trait governed by many small effect loci, most of which act at certain restricted developmental phases. This highlights the need for investigation of stage-specific growth affecting genes to elucidate important processes operating at different developmental phases.

show abstract

“…However, it has also been shown that alterations in cell-wall components other than lignin can affect biomass digestibility. For example, Penning et al have recently shown that quantitative trait loci (QTLs) for lignin abundance are independent of those for saccharification in a maize recombinant inbred population (14). In particular, altering cellulose production, deposition, or crystallinity can affect saccharification (15)(16)(17) because cellulose typically constitutes around one third of the total mass of plants and the insoluble crystalline cellulose fibers are hard to digest (18).…”

mentioning

confidence: 99%

Range of cell-wall alterations enhance saccharification in Brachypodium distachyon mutants

Marriott

Sibout

Lapierre

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Lignocellulosic plant biomass is an attractive feedstock for the production of sustainable biofuels, but the commercialization of such products is hampered by the high costs of processing this material into fermentable sugars (saccharification). One approach to lowering these costs is to produce crops with cell walls that are more susceptible to hydrolysis to reduce preprocessing and enzyme inputs. To deepen our understanding of the molecular genetic basis of lignocellulose recalcitrance, we have screened a mutagenized population of the model grass Brachypodium distachyon for improved saccharification with an industrial polysaccharide-degrading enzyme mixture. From an initial screen of 2,400 M 2 plants, we selected 12 lines that showed heritable improvements in saccharification, mostly with no significant reduction in plant size or stem strength. Characterization of these putative mutants revealed a variety of alterations in cell-wall components. We have mapped the underlying genetic lesions responsible for increased saccharification using a deep sequencing approach, and here we report the mapping of one of the causal mutations to a narrow region in chromosome 2. The most likely candidate gene in this region encodes a GT61 glycosyltransferase, which has been implicated in arabinoxylan substitution. Our work shows that forward genetic screening provides a powerful route to identify factors that impact on lignocellulose digestibility, with implications for improving feedstock for cellulosic biofuel production.lignocellulosic biofuel | matrix polysaccharides | lignin | feruloylation C oncerns over greenhouse-gas emissions and the sustainability of liquid transportation fuel supplies have led to a rapid expansion of global biofuel production in recent years. Current biofuel production is dominated by bioethanol produced by fermentation of starch or sucrose from food crops and by biodiesel produced by transesterification of plant or animal oils. Although the production of such "first-generation" biofuels can be efficient, there is widespread concern that further expansion will exacerbate anticipated problems with global food security through direct competition for resources. In addition, these crops often require high inputs and consequently have a relatively high carbon footprint (1). A promising alternative to first-generation biofuel is the use of nonfood lignocellulosic plant biomass, available as agricultural waste from food crops or produced from low input, nonfood plants such as perennial grasses (2).Lignocellulosic biomass is principally composed of plant secondary cell walls and comprises ∼70% polysaccharides, which can be converted into simple sugars for fermentation (3). The main challenge in producing bioethanol from lignocellulosic biomass is that these polysaccharides occur as part of a complex and indigestible macromolecular material that contains high amounts of lignin. The saccharification (conversion into simple sugars) of lignocellulose therefore requires energy demanding pretreatments and high enz...

show abstract

Genetic Determinants for Enzymatic Digestion of Lignocellulosic Biomass Are Independent of Those for Lignin Abundance in a Maize Recombinant Inbred Population

Cited by 51 publications

References 83 publications

Relationships between Biomass Composition and Liquid Products Formed via Pyrolysis

Relationships between Biomass Composition and Liquid Products Formed via Pyrolysis

Genetic variation of growth dynamics in maize (Zea mays L.) revealed through automated non‐invasive phenotyping

Range of cell-wall alterations enhance saccharification in Brachypodium distachyon mutants

Contact Info

Product

Resources

About