Genetic detection of colorectal cancer cells in circulation and lymph nodes

Nakamori, Shoji; Kameyama, Masao; Furukawa, Hiroshi; Takeda, Osamu; Sugai, Sachiko; Imaoka, Shingi; Nakamura, Yusuke

doi:10.1007/bf02062017

Cited by 50 publications

(25 citation statements)

References 17 publications

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“…Five studies were excluded as they were reviews or systematic reviews, which did not report on prognostic outcomes (Tsavellas et al, 2001;Sleijfer et al, 2007;Riethdorf et al, 2008;Sergeant et al, 2008;Tsouma et al, 2008). Ten studies were excluded as they did not report on prognostic outcomes (Denis et al, 1997;Nakamori et al, 1997;Douard et al, 2001;Guadagni et al, 2001;Patel et al, 2002;Silva et al, 2002;Yokoyama and Yamaue, 2002;Schuster et al, 2004;Yeh et al, 2006;Lagoudianakis et al, 2009). The outcomes measured in the 14 studies included in this review were too heterogeneous with regards to the methodology of data collection to allow quantitative analysis.…”

Section: Resultsmentioning

confidence: 99%

Prognostic significance of circulating tumour cells following surgical resection of colorectal cancers: a systematic review

et al. 2010

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Background: The role of adjuvant chemotherapy after resection of colorectal cancers (CRCs) is well understood for patients with stage-I or stage-III disease. Its efficacy for those with stage-II disease remains much less clear. Many investigators have sought to identify prognostic markers that might clarify which patients have the highest risk of recurrence and would, therefore, be most likely to benefit from chemotherapy. This systematic review examines evidence for the use of peripherally sampled, circulating tumour cells (CTCs) as such a prognostic marker. Methods: A comprehensive literature search was used to identify studies reporting on the significance of CTCs in the postoperative blood of CRC patients. Results: Fourteen studies satisfied the inclusion criteria. Six of the nine studies that took blood samples 24 h or more postoperatively found detection of postoperative CTCs to be an independent predictor of cancer recurrence. Conclusion: The presence of CTCs in peripheral blood at least 24 h after resection of CRCs is an independent prognostic marker of recurrence. Further studies are needed to clarify the optimal time point for blood sampling and determine the benefit of chemotherapy in CTC-positive patients with stage-II disease.

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Section: Resultsmentioning

confidence: 99%

Prognostic significance of circulating tumour cells following surgical resection of colorectal cancers: a systematic review

et al. 2010

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“…We speculate that changes in expression of CK19 in basal cells are involved in malignant transformation and cancer proliferation. It has been suggested that, clinically, the detection of blood or urinary CK19 in breast cancer 5) , lung cancer 14) , stomach cancer 24) , or colon cancer 11) is useful in the early diagnosis of cancer metastasis. The present results also suggest CK14 and 19 as a potential marker of malignant change in the oral cavity.…”

Section: Discussionmentioning

confidence: 99%

“…These proteins are classified into two subfamilies: type I acidic (CK9-20); and type II, neutral or basic (CK1-8) 3,4,8) . Cytokeratin 19 is characterized by its expression and localization, and has been utilized as an indicator gene in peripheral blood cancer cells in the diagnosis of breast 5) , lung 14) , stomach 24) , and colorectal cancer 11) . In addition, an attempt was made to evaluate squamous epithelial carcinomas based on localization of CK 10,18) .…”

Section: Introductionmentioning

confidence: 99%

Expression of Cytokeratin 14 and 19 in Process of Oral Carcinogenesis

Yoshida

Sato

Tonogi

et al. 2015

Bull. Tokyo Dent. Coll.

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Cytokeratins (CK) are abundant in keratinized cells, particularly CK14 and CK19, which are expressed in stratified squamous epithelial cells. In this study, expression of CK14 and 19 was examined in human epithelial and dysplastic tissues. Surgical specimens from patients with clinically diagnosed oral leukoplakia or early cancer were stained with hematoxylin and eosin and classified into normal, low grade dysplasia (LGD), high grade dysplasia (HGD), or squamous cell carcinoma (SCC). The sections were examined by immunostaining and reverse transcription-polymerase chain reaction (RT-PCR) for CK14 and CK19. Expression and the results of RT-PCR for CK14 showed a decrease in the order of LGD, HGD, and SCC, whereas those of CK19 showed an increase in that order. These results suggest that decreased expression of CK14 and increased expression of CK19 serve as indicators of potential for malignant transformation.

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“…Thus, various methods have been proposed to detect micrometastasis in the dissected lymph nodes. 18,19 Molecular biological techniques, such as immunohistochemical staining and expression of mRNA against cancer-related antigen, have shown that an RT-PCR assay could be one of the most sensitive and accurate methods of detecting cancer cells. [3][4][5][19][20][21] In the present investigation, we used CEA mRNA to detect the spread of epithelial cancer cells.…”

Section: Discussionmentioning

confidence: 99%