Genetic Dependence of IgE Antibody Production in Mice Infected with the Nematode <i>Nippostrongylus brasiliensis</i>

Abstract

We investigated the IgE-antibody response in 36 inbred mouse strains during infection with Nippostrongylus brasiliensis. With regard to the N. brasiliensis-specific IgE-antibody activity responder and nonresponder mice were obtained. Mice with the H-2-f haplotype (A.CA, B10.M, A.TFR 5) are high specific responded. It is suggested that one Ir gene for the N. brasiliensis-induced IgE-antibody response is localized within the K to J region of the mouse major histocompatibility complex. To obtain a medium IgE-antibody response it appears that a complementation between two Ir genes is necessary. An additional genetic control beyond the H-2 complex cannot be excluded. As to the total nonspecific serum IgE levels the parasitic infection leads to a 20- to 40-fold increase in high and low specific responder strains as well. Pretreatment of high responder mice (A.TFR 5, B10.M) with sera that were obtained from normal or complete Freund’s adjuvant treated low (B10.G) and nonresponder (A.TFR 1) mice led to significant inhibition or enhancement of the N. brasiliensis-specific IgE-antibody response.

Immunological Alterations Inducible by Mercury Compounds

“…In all three strains, the serum IgM concentrations remained un changed. The HgCL doses used in the repeat adminis tration regime of the present study are clearly below toxic concentrations [22], The IgG and IgM levels detected before the onset of HgCL treatment and in control mice are in the range also reported by others [13,25,26], and the same is true for the IgE levels [27,28] although two re cent publications [26,29] reported considerably lower IgE concentrations. While IgM and IgG levels were very similar in the three mouse strains studied by us, normal IgE levels in strains A.SW and DBA/2 con sistently differed by a factor of 4.…”

“…Nevertheless, even the 8-to 30-fold in crease in total IgE and the 7-to 30-fold increase in to tal IgG detected in HgCL-treated A.SW mice repre sent very significant deviations from normal when compared with other murine models of IgE induction or polyclonal B cell stimulation. Thus, in mice in fected with the nematode Nippostrongylus brasiliensis total serum IgE increases to 20-to 30-fold [27], respec tively up to 100-fold [26] above normal. However, during the chronic graft-versus-host reaction in mice, a powerful model of T cell-dependent B cell activa tion and systemic autoimmune disease, total serum IgG increases only 2-fold [35,36].…”

Generation of Cell-Derived Factors in vitro Which Modulate IgE Synthesis

“…Suppression of IgE syn- (table I). In a first series of experiments, the HMWCS from A.b.-reinfected Bald/c cells, which had been stimu lated with monoclonal IgE in vitro, was injected into A.CA mice -high responders with regard to the A.¿.-specific IgE response [9], At 12-hour intervals mice received 4 injections with 0.5 mg each of the HMW fraction. Treatment of mice was performed on days-1 and 0; on days 0 and 28 the mice were infected with 750 larvae of N.b.…”

Nematode infection induces TH2 cell-associated immune responses in LEC mutant rats with helper T cell immunodeficiency

“…Specific IgE was undetectable in LEC rats and very low in LEA rats. The reason for the lack of specific IgE in both strains is not clear, but it has been found that induction of nematode-specific IgE varies significantly among animal strains and some strains show no detectable specific IgE despite significant production of non-specific antibodies of this isotype (Pfeiffer, König & Bohn 1983, Uchikawa et al 1996.…”