Genetic delineation of sibling species of the pest fruit fly Bactocera (Diptera: Tephritidae) using microsatellites

Abstract

Using a large set of microsatellites, the genetic relationships between three closely related Australian fruit fly species, Bactrocera tryoni (Froggatt), B. neohumeralis (Hardy) and B. aquilonis(May) were investigated. Bactrocera tryoni and B. neohumeralis are sympatric, while B. aquilonisis allopatric to both. The sympatric species, B. tryoni and B. neohumeralis, were found to be genetically distinct. It is likely that despite differences in mating time between these two species, some gene flow still occurs. In contrast, the sibling species B. tryoni and B. aquilonis were found to be closely related, despite allopatry. The level of genetic divergence was similar to that found within eastern Australian populations of B. tryoni. Consideration of all available genetic data suggests that this similarity is not due to recent (i.e. within the last 30 years) displacement of B. aquilonis by B. tryoni from the B. aquilonis region (north-western Australia). Instead the data suggests that, at least in the areas sampled, asymmetrical hybridization may have occurred over a longer timescale.

Genetic relations between outbreaks of the Queensland fruit fly, Bactrocera tryoni (Froggatt) (Diptera: Tephritidae), in Adelaide in 2000 and 2002

“…The microsatellites used are detailed in Kinnear et al . (1998) and Wang et al (2003). We tested for clustering among the outbreak flies using both correspondence analysis (CA) and the Structure software (Pritchard et al .…”

DNA microsatellite analysis of naturally occurring colour intermediates between Bactrocera tryoni (Froggatt) and Bactrocera neohumeralis (Hardy) (Diptera: Tephritidae)

“…With microsatellite markers having been isolated for these species (Kinnear et al 1998;Wang et al 2003), we revisited the question of the origin of these wild intermediates. An earlier study has shown that the two species can be differentiated using microsatellites, but that they are closely related (F ST ∼ 0.1; Wang et al 2003).…”

GENETIC DIVERSITY OF NATURAL POPULATIONS OF Bactrocera occipitalis (Bezzi) AND B. philippinensis Drew and Hancock (Diptera: Tephritidae) IN SELECTED MANGO PRODUCING AREAS IN THE PHILIPPINES USING MICROSATELLITES

“…This means that on the average, the populations were 49 % deficient of heterozygotes. HWE deviations as a result of heterozygote deficiency may be attributed to null alleles, inbreeding, finite sample size, a chance of sampling effect or the combination of subpopulations (Aketarawong et al, 2006;Wang et al, 2003;Dai et al, 2004;Augustinos et al, 2005). Some samples failed to yield PCR products at loci Bd39, Bd43B, and Bd76 but they were successfully amplified at other loci, suggesting the existence of null alleles at these loci.…”