Genetic data for PowerPlex 21™ autosomal and PowerPlex 23 Y-STR™ loci from population of the state of Uttar Pradesh, India

Srivastava, Ankit; Kumawat, R. K.; Dixit, Shivani; Kaitholia, Kamlesh; Shrivastava, Divya; Yadav, V. K.; Nigam, Kriti; Sharma, Harsh; Trivedi, Veena Ben; Chaubey, Gyaneshwer; Shrivastava, Pankaj

doi:10.1007/s00414-018-01993-z

Cited by 24 publications

(5 citation statements)

References 10 publications

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“…DNA phenotyping is now attracting the attention of the professionals, scientists (Butler 2015a, b;Shrivastava et al 2016). In India, recently several population studies based on STR markers have been published (Srivastava et al 2019a;Srivastava etal. 2019b;Shrivastava et al 2020).…”

Section: Development Of the Dna Technologymentioning

confidence: 99%

Impact of DNA evidence in criminal justice system: Indian legislative perspectives

Srivastava

Harshey

Das

et al. 2022

Egypt J Forensic Sci

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Background The scientific and technological advances along with the changing socio-economical standards of society have posed new challenges to the criminal justice system. With the changes in society and technology, there is also an increase in crime rate. Forensics provides the scientific proofs beyond the shadow of reasonable doubt and thus significantly contributes in criminal as well as civil investigations and legal matters. Main body of the abstract Discovery of DNA has opened new avenues and the advancement of DNA technology and its introduction into the court of law has provided extensive aid in the resolution of civil and criminal disputes. In India, the DNA technology was first introduced in a paternity dispute in 1989. However, the need for legislation and guidelines to support grounds for the use of DNA profiling for forensic purposes in India has been recognized for some time now. In July 2019, the DNA Technology (Use and Application) Bill 2019 was introduced in the Indian parliament. Short conclusion Herein, this article is focused on the current aspects of DNA based evidence in the Indian Criminal Justice system along with the associated issues, highlighting the need for specific DNA based legislation.

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Section: Development Of the Dna Technologymentioning

confidence: 99%

Impact of DNA evidence in criminal justice system: Indian legislative perspectives

Srivastava

Harshey

Das

et al. 2022

Egypt J Forensic Sci

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“…The genetic dataset of present study was compared with the dataset of reported indigenous populations viz., Balmiki (Punjab) 29 , Sakaldwipi Brahmin (Jharkhand) 29 , Konkanastha_Brahmin (Maharashtra) 29 , Iyengar (Tamilnadu) 29 , Kurumans (Tamilnadu) 29 , Munda (Jharkhand) 29 , Chenchu (Andhra Pradesh) 30 , Lambadi (Andhra Pradesh) 30 , Naikpod_Gond (Andhra Pradesh) 30 , Yerukula (Andhra Pradesh) 30 , Munda (Chotanagpur) 31 , Santal (Chotanagpur) 31 , Oraon (Chotanagpur) 31 , Lodha (Bengal) 32 , Kora (Bengal) 32 , Maheli (Bengal) 32 , Central Indian Population 33 , Gond (MP) 34 , Gond_2 (MP) 35 , Gond_1 (MP) 29 , Oraon (Chhattishgarh) 36 , Population of Jharkhand 37 , Jat Sikh (Punjab) 38 , Baniya (Punjab) 38 , Khatri (Punjab) 38 , Population of Uttar Pradesh 39 , Kahar population (UP) 40 , Himachal Pradesh Population 18 , Gorkhas 41 , Tamil Population (Tamil Nadu) 42 , Population of Rajasthan 43 , Population of Odisha 44 and Bhuiyan Population (Jharkhand) 45 ;and global populations namely; Manchu Population of China 46 Medical College, Jhansi is part of Bundelkhamd University, Jhansi. One of the co-author of this manuscript is posted in the same university and we took the ethical clearance on our proposal from the same Institute which is part of Bundelkhamd University, Jhansi,UP,India.…”

Section: Genetic Data Of Reported Populations For Comparisonmentioning

confidence: 99%

Genomic Insight Into 21 Hypervariable Molecular Markers in The Population of Lower Himalayan Geographical Province Himachal Pradesh, India

Sharma

Srivast

Sahajpal

et al. 2021

Preprint

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A natural geographical barrier between Tibetan plateau and south Asian countries, the Himalayan Mountain range, serves as a natural barrier between these regions for genetic flow. This has lead to vast genomic divergence among the population residing in Indian Himalayan region..This study was designed with an aim to decipher the genomic diversity and molecular characterization of 21 hypervariable molecular markers in the population of geographical province of Himachal Pradesh in the lower Himalayan region. Randomly selected 401 unrelated individuals native of the lower Himalayan geographical region were included for this study. 21 hypervariable molecular markers included in the PowerPlex® 21 system were amplified and genotyped. A total of 246 alleles and 12.3 (SE 0.927) private alleles per locus were observed. Population differentiation analysis revealed that the studied population showed a genetic affinity with the population of North India, North-west India, Central India, and Uttar Pradesh rather than the population of East India, South India, East Asia, and West Asia. Heterozygosity at the studied loci was found in the range of 0.686 to 0.920. The unite discrimination power (PD) and exclusion power (PE) was found to be 1 and 0.999999998073765 respectively. The unite value of matching probability and typical paternity index was found to be 9.33x10-26 and 5.05x108 respectively for the studied population. All the tested loci were found in Hardy Weinberg Equilibrium (HWE) expectations. Overall the studied population exhibited a great extent of genomic diversity and had a greater genetic affinity with Indo-European speakers rather than Dravidian and Tibeto-Burman speakers.

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“…A statistical analysis showed that the majority of microhaplotypes sequenced were highly polymorphic and informative in the Gansu Han population. The CMPs for most commercial forensic STR kits range from 10 −17 to 10 −26 [25][26][27] . In this study, the CMP for 106 microhaplotypes was 5.23 × 10 −66 , which is tens of orders of magnitude lower than those of STR multiplex systems.…”

Section: Population Datamentioning

confidence: 99%

A 124-plex Microhaplotype Panel Based on Next-generation Sequencing Developed for Forensic Applications

Pang

Rao

Chen

et al. 2020

Sci Rep

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Microhaplotypes are an emerging type of forensic genetic marker that are expected to support multiple forensic applications. Here, we developed a 124-plex panel for microhaplotype genotyping based on next-generation sequencing (NGS). The panel yielded intralocus and interlocus balanced sequencing data with a high percentage of effective reads. A full genotype was determined with as little as 0.1 ng of input DNA. Parallel mixture experiments and in-depth comparative analyses were performed with capillary-electrophoresis-based short tandem repeat (STR) and NGS-based microhaplotype genotyping, and demonstrated that microhaplotypes are far superior to STRs for mixture deconvolution. DNA from Han Chinese individuals (n = 256) was sequenced with the 124-plex panel. In total, 514 alleles were observed, and the forensic genetic parameters were calculated. A comparison of the forensic parameters for the 20 microhaplotypes with the top A e values in the 124-plex panel and 20 commonly used forensic STRs showed that these microhaplotypes were as effective as STRs in identifying individuals. A linkage disequilibrium analysis showed that 106 of the 124 microhaplotypes were independently hereditary, and the combined match probability for these 106 microhaplotypes was 5.23 × 10 −66. We conclude that this 124-plex microhaplotype panel is a powerful tool for forensic applications. The microhaplotype is a powerful new type of forensic genetic marker 1,2. It is the combination of two or more closely linked single-nucleotide polymorphisms (SNPs) within DNA segments of 200 base pairs (bp), and offers multiple forensic applications 3-7. Short tandem repeat (STR) genotyping is currently the dominant technology in forensic DNA laboratories. Although it works well with single-sourced DNA samples, great challenges are encountered with DNA mixtures because stutters in the major donor DNA can be indistinguishable from alleles in the minor donor DNA 3,8. Stutters are unavoidable during the replication of repetitive DNA, and they severely interfere with mixture deconvolution. SNPs are not repetitive sequences, but are typically biallelic, which restricts their utility in the analysis of mixtures. Microhaplotypes have the advantages of both STRs and SNPs because they are multiallelic and do not produce stutters during amplification. Therefore, microhaplotypes are perfect genetic markers for mixture deconvolution. Although capillary electrophoresis (CE)-based genetic analyzers are widely used in forensic DNA laboratories, these machines are unsuitable for microhaplotype genotyping 8. Several methods have been used for microhaplotype detection. TaqMan assays have been used to type each SNP that constitutes a microhaplotype 8 , followed by a PHASE software analysis to determine the cis/trans relationships between individual SNP alleles. Single-strand conformational polymorphisms 9 and high-resolution melting curves 4 have also been used for microhaplotype genotyping. These methods are simple and inexpensive, but they can pose problems when multipl...

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Genetic data for PowerPlex 21™ autosomal and PowerPlex 23 Y-STR™ loci from population of the state of Uttar Pradesh, India

Cited by 24 publications

References 10 publications

Impact of DNA evidence in criminal justice system: Indian legislative perspectives

Impact of DNA evidence in criminal justice system: Indian legislative perspectives

Genomic Insight Into 21 Hypervariable Molecular Markers in The Population of Lower Himalayan Geographical Province Himachal Pradesh, India

A 124-plex Microhaplotype Panel Based on Next-generation Sequencing Developed for Forensic Applications

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