Genetic damage induced by lead chloride in different tissues of fresh water climbing perch Anabas testudineus (Bloch)

Ahmed, Md. Kawser; Parvin, Elora; Arif, Murtaza; Islam, Monirul; Akter, Mosammat Salma; Khan, Mohammad Rezwan

doi:10.1007/s10661-010-1869-6

Cited by 8 publications

(5 citation statements)

References 34 publications

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“…2). The current results are in agreement with the previous research revealing damage to DNA in terms of tail length in gills and liver due to heavy metals pollution overload in different fish species like B. barbus 55,56 , A. testudineus 57 , and mullet and sea bass 58 . It has been reported that when ROS production surpasses the fish defense system due to excessive overload of heavy metals/xenobiotics and comes in contact with DNA and form adducts, it ultimately leads to cellular lesions or DNA damage 6,59 .…”

Section: Genotoxicitysupporting

confidence: 93%

Labeo rohita, a bioindicator for water quality and associated biomarkers of heavy metal toxicity

et al. 2021

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This study investigated the effect of heavy metals on Labeo rohita inhabiting the Yamuna River, India. Levels of heavy metals measured in the water were as follows: Fe>Mn>Zn>Cu>Ni>Cr>Cd. Gill and liver tissue of exposed L. rohita showed a high metal pollution index, compared to reference fish collected from the Agra Canal. In the exposed fish, higher levels of creatinine and enzyme activity (alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase) were observed, while the A:G index declined. Additionally, higher TLC, lymphocytes, respiratory burst, and nitric oxide synthase activity indicated a heightened immune response. Levels of superoxide dismutase and lipid peroxidation were elevated, while catalase, glutathione S transferase, and glutathione was reduced. DNA of the exposed fish appeared deteriorated, with a greater mean tail length in comparison to the reference. Our results imply that Yamuna River water generates oxidative stress and DNA damage in L. rohita. As this river is a critical source of water and food to the native community, this could pose a threat to public health similar to that in the indicator organism.

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Section: Genotoxicitysupporting

confidence: 93%

Labeo rohita, a bioindicator for water quality and associated biomarkers of heavy metal toxicity

et al. 2021

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“…Besides zebrafish as the fish model that lives in a freshwater environment, several other predominantly freshwater as well as brackish water species are used for the assessment of different contaminants using the comet assay; they include brown trout (Salmo trutta fario) [85], marble trout (Salmo marmoratus) [124], trout (Salmo cenerinus) [124], rainbow trout (Oncorhynchus mykiss) [125,126], common carp (Cyprinus carpio) [76, 82,127], major carp (Catla catla) [128], mrigal carp (Cirrhinus mrigala) [128], gibel carp (Carassius auratus gibelio) [129], common barbell (Barbus barbus) [130], common bleak (Alburnus alburnus) [131,132], freshwater bream (Abramis brama) [133], silver bream (Abramis bjoerkna) [134], white-eye bream (Abramis sapa) [134], rohu (Labeo rohita) [135][136][137][138][139], orangefin labeo (Labeo calbasu) [86], brown bullhead (Ameiurus nebulosus) [76,140], chub (Leuciscus cephalus) [141][142][143], European chub (Squalius cephalus) [87,[144][145][146], pale chub (Zacco platypus) [147], climbing perch (Anabas testudineus) [148], small-scaled pacu (Piaractus mesopotamicus) [149,150], eastern mosquitofish (Gambusia holbrooki) [77], Nile tilapia (Oreochromis niloticus) [151][152][153]…”

Section: Accepted Manuscriptmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.

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“…Similarly to our data, maximal increase reached approximately a 2.7-fold increase as compared to controls and no differences were observed between the exposure time periods. More recently, in the freshwater fish Prochilodus lineatus treated with 5 mg/L lead nitrate for 6 to 96 h, Monteiro et al (2011) reported that while the comet assay showed a genotoxic effect in blood, gill, and liver cells only after 96 h, MNi frequency did not increase at any exposures; nonetheless, the frequency of other erythrocyte nuclear abnormalities showed a significant increase after 24 and 96 h. Likewise, by means of the comet assay, concentration-dependent DNA damage was shown in the gills, kidney, and liver of freshwater climbing perch Anabas testudineus exposed to 0.1-2 mg/L lead chloride for 96 h (Ahmed et al 2011b).…”

Section: Discussionmentioning

confidence: 87%

“…The MN test can be carried out in any proliferating active tissue. The blood, gill, liver, kidney, and fin cells have been mainly used (Al-Sabti and Metcalfe 1995;Cavas et al 2005;Udroiu 2006;Cavaş 2008;Ahmed et al 2011b). However, peripheral erythrocytes are the most commonly employed cells in the piscine MN test because it avoids the complex cellular dissociation required in other tissues, which may lead to mechanical stress and cell damage, as well as the killing of the animals (Bolognesi et al 2006;Cavaş 2008).…”

Section: Introductionmentioning

confidence: 99%

Early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in European seabass, Dicentrarchus labrax

Canalejo

Díaz-de-Alba

Granado-Castro

et al. 2015

Environ Sci Pollut Res

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Cu, Pb, and As, which are among the most abundant metals in the aquatic environment, are also among the most health-threatened by causing diverse cellular injuries. The aim of this study was to assess and compare the potential early induction of genotoxic effects after waterborne Cu, Pb, and As exposure in European seabass, Dicentrarchus labrax, a commercial widely cultured fish, using the micronucleus (MN) assay in peripheral blood erythrocytes. Fish were exposed under laboratory conditions to nominal solutions ranging 0-10 mg/L for 24 and 96 h. Furthermore, actual metal ion concentrations were measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES) or differential pulse anodic stripping voltammetry (DPASV) in water and four fish tissues differentially related to environmental exposition and metal accumulation, i.e. the gills, liver, muscle, and brain. Dose-dependent increases of micronuclei (MNi) frequency were observed after these very short exposures; based on measured metal concentrations in water, the genotoxic effect ordered as Cu > As > Pb. Significant genotoxic effect at 0.009 mg/L Cu, 0.57 mg/L Pb, and 0.01 mg/L As was seen. For Cu and Pb these are only slightly higher, but for As it is notably lower than the USEPA criteria of maximum concentration to prevent acute toxicity in aquatic organisms.Furthermore, genotoxicity was differentially related to metal accumulation. MNi frequency correlated positively with the content of Pb in all the organs, with the content of As in liver and gills and only with the content of Cu in the brain. In conclusion, our findings raised environmental concerns because these depicted a genotoxic potential of Cu, Pb, and As after a very short exposure to low but environmentally relevant concentrations, too close to regulatory thresholds. In addition, the MN test in D. labrax could be considered an early biomarker of genotoxicity induced by these metals in fish.

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Genetic damage induced by lead chloride in different tissues of fresh water climbing perch Anabas testudineus (Bloch)

Cited by 8 publications

References 34 publications

Labeo rohita, a bioindicator for water quality and associated biomarkers of heavy metal toxicity

Labeo rohita, a bioindicator for water quality and associated biomarkers of heavy metal toxicity

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in European seabass, Dicentrarchus labrax

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