Genetic Damage in
            <i>Escherichia coli</i>
            K12 AB2480 by Broad-spectrum Near-Ultraviolet Radiation

Rb, Webb; Brown,

doi:10.1126/science.6760398

Cited by 10 publications

(2 citation statements)

References 19 publications

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“…Some contributions use mutagenicity and genetic approaches to identify near-UV (NUV, 320–400 nm) radiation effects on bacteria cells. Webb and Brown demonstrated that irradiation with either broad-spectrum NUV or monochromatic wavelengths in the NUV can cause specific damages to DNA in E. coli K12 AB248040. Eisenstark studied the DNA mutations due to NUV in E. coli .…”

Section: Discussionmentioning

confidence: 99%

Intracellular mechanisms of solar water disinfection

Castro-Alférez

Polo-López

Fernández‐Ibáñez³

2016

Sci Rep

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Solar water disinfection (SODIS) is a zero-cost intervention measure to disinfect drinking water in areas of poor access to improved water sources, used by more than 6 million people in the world. The bactericidal action of solar radiation in water has been widely proven, nevertheless the causes for this remain still unclear. Scientific literature points out that generation of reactive oxygen species (ROS) inside microorganisms promoted by solar light absorption is the main reason. For the first time, this work reports on the experimental measurement of accumulated intracellular ROS in E. coli during solar irradiation. For this experimental achievement, a modified protocol based on the fluorescent probe dichlorodihydrofluorescein diacetate (DCFH-DA), widely used for oxidative stress in eukaryotic cells, has been tested and validated for E. coli. Our results demonstrate that ROS and their accumulated oxidative damages at intracellular level are key in solar water disinfection.

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Section: Discussionmentioning

confidence: 99%

Intracellular mechanisms of solar water disinfection

Castro-Alférez

Polo-López

Fernández‐Ibáñez³

2016

Sci Rep

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“…Other lesions such as the (6–4) pyrimidine–pyrimidone photoproducts are even more proficient in stopping DNA synthesis (57). The distribution of photoproducts accumulating in cells upon UV irradiation is different for different UV regions and also varies between vegetative cells and spores (3,24,33,58–70). In vegetative cells dimers predominate in bacterial DNA after exposure to UVC.…”

Section: Uv Damage and Repairmentioning

confidence: 99%

Bacterial Inactivation by Solar Ultraviolet Radiation Compared with Sensitivity to 254 nm Radiation

Coohill

Sagripanti

2009

Photochem & Photobiology

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Our goal was to derive a quantitative factor that would allow us to predict the solar sensitivity of vegetative bacterial cells to natural solar radiation from the wealth of data collected for cells exposed to UVC (254 nm) radiation. We constructed a solar effectiveness spectrum for inactivation of vegetative bacterial cells by combining the available action spectra for vegetative cell killing in the solar range with the natural sunlight spectrum that reaches the ground. We then analyzed previous studies reporting the effects of solar radiation on vegetative bacterial cells and on bacterial spores. Although UVC-sensitive cells were also more sensitive to solar radiation, we found no absolute numerical correlation between the relative solar sensitivity of vegetative cells and their sensitivity to 254 nm radiation. The sensitivity of bacterial spores to solar exposure during both summer and winter correlated closely to their UVC sensitivity. The estimates presented here should make it possible to reasonably predict the time it would take for natural solar UV to kill bacterial spores or with a lesser degree of accuracy, vegetative bacterial cells after dispersion from an infected host or after an accidental or intentional release.

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Effects of sunlight and autochthonous microbiota onEscherichia coli survival in an estuarine environment

Rhodes

Kator

1990

Current Microbiology

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Genetic Damage in Escherichia coli K12 AB2480 by Broad-spectrum Near-Ultraviolet Radiation

Cited by 10 publications

References 19 publications

Intracellular mechanisms of solar water disinfection

Intracellular mechanisms of solar water disinfection

Bacterial Inactivation by Solar Ultraviolet Radiation Compared with Sensitivity to 254 nm Radiation

Effects of sunlight and autochthonous microbiota onEscherichia coli survival in an estuarine environment

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