Genetic Control of Seed Shattering in Rice by the APETALA2 Transcription Factor <i>SHATTERING ABORTION1</i>

Zhou, Yan; Lu, Dan; Li, Canyang; Luo, Jun; Zhu, Ben-Zhan; Zhu, Jingjie; Shangguan, Yingying; Wang, Zixuan; Su, Tao; Zhou, Bo; Han, Bin

doi:10.1105/tpc.111.094383

Cited by 198 publications

(242 citation statements)

References 46 publications

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“…POS1 shared a similar expression pattern with the Arabidopsis AP2 (ref. 28), and its putative orthologues in Antirrhinum majus 29 , Zea mays 30 and Oryza sativa 31 . However, POS1 did not ARTICLE specify floral organ identity but controlled the floral organ size, berry size and seed size in tomatillo.…”

Section: Article Nature Communications | Doi: 101038/ncomms5271mentioning

confidence: 99%

Regulatory change at Physalis Organ Size 1 correlates to natural variation in tomatillo reproductive organ size

Wang

et al. 2014

Nat Commun

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The genetic basis of size variation in the reproductive organs of tomatillo (Physalis philadelphica) is unknown. Here we report that the expression levels of the gene Physalis Organ Size 1 (POS1) are positively associated with size variation in P. philadelphica reproductive organs such flowers, berries and seeds. POS1 knockdown results in smaller flowers and berries with smaller cells as compared with their wild-type counterparts. Conversely, POS1 overexpression promotes organ size without increasing the cell number. The first introns of the POS1 alleles from the large, intermediate and small tomatillo groups contain one, two and three 37-bp repeats, respectively. Furthermore, our results show that copy variation of repeats in the first intron of POS1 alleles results in differential expression of this gene. Thus, co-variation in tomatillo reproductive organ sizes can be attributed to the novel regulatory variation in POS1.

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Section: Article Nature Communications | Doi: 101038/ncomms5271mentioning

confidence: 99%

Regulatory change at Physalis Organ Size 1 correlates to natural variation in tomatillo reproductive organ size

Wang

et al. 2014

Nat Commun

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“…Seed dispersal is affected by the development of the abscission zone (AZ) and lignification (Lewis et al, 2006;Estornell et al, 2013;Dong et al, 2014;Dong and Wang, 2015). Differentiated AZ cells are smaller and isodiametrically compacted when compared with the surrounding cells (Zhou et al, 2012). In rice (Oryza sativa), the AZ develops between the sterile lemma and rudimentary glume when panicles are 5 to 30 mm long (Ji et al, 2010).…”

mentioning

confidence: 99%

“…Expression of the former is detected in young spikelets, especially within the AZ, lamina joint, and intercalary meristem (IM) region. SH5 induces two transcription factor genes, SHAT1 and Sh4, that are essential for appropriate AZ development (Li et al, 2006;Zhou et al, 2012;Yoon et al, 2014). Although SH5 is unable to induce the formation of a proper AZ, the gene causes grain shattering by repressing lignin deposition in the pedicel region (Yoon et al, 2014).…”

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confidence: 99%

KNOX Protein OSH15 Induces Grain Shattering by Repressing Lignin Biosynthesis Genes

et al. 2017

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Seed shattering is an agronomically important trait. Two major domestication factors are responsible for this: qSH1 and SH5. Whereas qSH1 functions in cell differentiation in the abscission zone (AZ), a major role of SH5 is the repression of lignin deposition. We have determined that a KNOX protein, OSH15, also controls seed shattering. Knockdown mutations of OSH15 showed reduced seed-shattering phenotypes. Coimmunoprecipitation experiments revealed that OSH15 interacts with SH5 and qSH1, two proteins in the BELL homeobox family. In transgenic plants carrying the OSH15 promoter-GUS reporter construct, the reporter gene was preferentially expressed in the AZ during young spikelet development. The RNA in situ hybridization experiment also showed that OSH15 messenger RNAs were abundant in the AZ during spikelet development. Analyses of osh15 SH5-D double mutants showed that SH5 could not increase the degree of seed shattering when OSH15 was absent, indicating that SH5 functions together with OSH15. In addition to the seedshattering phenotype, osh15 mutants displayed dwarfism and accumulated a higher amount of lignin in internodes due to increased expression of the genes involved in lignin biosynthesis. Knockout mutations of CAD2, which encodes an enzyme for the last step in the monolignol biosynthesis pathway, caused an easy seed-shattering phenotype by reducing lignin deposition in the AZ. This indicated that the lignin level is an important determinant of seed shattering in rice (Oryza sativa). Chromatin immunoprecipitation assays demonstrated that both OSH15 and SH5 interact directly with CAD2 chromatin. We conclude that OSH15 and SH5 form a dimer that enhances seed shattering by directly inhibiting lignin biosynthesis genes.

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“…SHATTERING ABORTION1 (SHAT1) is an AP2-like transcription factor responsible for abscission zone differentiation in rice [73]. SHAT1 expression is promoted by Sh4, and qSH1 is upregulated by SHAT1 and Sh4 to maintain the abscission zone development.…”

Section: A Shattering In Cereal Cropsmentioning

confidence: 99%

“…SHAT1 expression is promoted by Sh4, and qSH1 is upregulated by SHAT1 and Sh4 to maintain the abscission zone development. Collectively, SH4, SHAT1, and qSH1 are involved in a positive feedback mechanism that maintains the abscission zone development and differentiation [73].…”

Section: A Shattering In Cereal Cropsmentioning

confidence: 99%

Pod Shattering: A Homologous Series of Variation Underlying Domestication and an Avenue for Crop Improvement

Ogutcen¹,

Pandey²,

Khan³

et al. 2018

Preprint

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In wild habitats, fruit dehiscence is a critical strategy for seed dispersal; however, in cultivated crops it is one of the major sources of yield loss. Therefore, indehiscence of fruits, pods, etc., was likely to be one of the first traits strongly selected in crop domestication. Even with the historical selection against dehiscence in early domesticates, it is a trait still targeted in many breeding programs, particularly in minor or underutilized crops. Here, we review of this trait in pulse (grain legume) crops, which are of growing importance as a source of protein in human and livestock diets, and which have received less attention iii) the molecular pathways underlying this important trait, iv) an overview of the extent of crop losses due to shattering, and the effects of environmental factors on shattering, and, v) efforts to reduce shattering in crops. While our focus is mainly pulse crops, we also included comparisons to crucifers and cereals because there is extensive research on shattering in these taxa.

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Genetic Control of Seed Shattering in Rice by the APETALA2 Transcription Factor SHATTERING ABORTION1

Cited by 198 publications

References 46 publications

Regulatory change at Physalis Organ Size 1 correlates to natural variation in tomatillo reproductive organ size

Regulatory change at Physalis Organ Size 1 correlates to natural variation in tomatillo reproductive organ size

KNOX Protein OSH15 Induces Grain Shattering by Repressing Lignin Biosynthesis Genes

Pod Shattering: A Homologous Series of Variation Underlying Domestication and an Avenue for Crop Improvement

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