Genetic control of resistance to enterotoxigenic Escherichia coli in infant mice

Duchet-Suchaux, Marion; Menanteau, Pierrette; Roux, Hervé Le; Elsen, Jean-Michel; Lechopier, Patrick

doi:10.1016/0882-4010(92)90075-y

Cited by 6 publications

(2 citation statements)

References 12 publications

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“…One explanation for this phenomenon may be that the mice used in our experiments were genetically heterogenous outbred animals, making some variation in susceptibility to ETEC likely. Studies of infant mice have shown that there is considerable phenotypic variation with respect to susceptibility to ETEC or its toxins among different inbred strains of mice (3,17,18), indicating that host genetic factors may play a significant role in the acquisition of ETEC infections.…”

Section: Discussionmentioning

confidence: 99%

Importance of Heat-Labile Enterotoxin in Colonization of the Adult Mouse Small Intestine by Human EnterotoxigenicEscherichia coliStrains

Allen¹,

Randolph²,

2006

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Enterotoxigenic Escherichia coli (ETEC) infections are a significant cause of diarrheal disease and infant mortality in developing countries. Studies of ETEC pathogenesis relevant to vaccine development have been greatly hampered by the lack of a suitable small-animal model of infection with human ETEC strains. Here, we demonstrate that adult immunocompetent outbred mice can be effectively colonized with the prototypical human ETEC H10407 strain (colonization factor antigen I; heat-labile and heat-stable enterotoxin positive) and that production of heat-labile holotoxin provides a significant advantage in colonization of the small intestine in this model.Enterotoxigenic Escherichia coli (ETEC) infections are a significant cause of diarrheal disease worldwide. Infections caused by this heterogeneous group of pathogens remain major causes of diarrheal morbidity and infant mortality in developing countries (11,33,61), are perennially associated with disease in travelers (4, 7, 43) and in soldiers deployed to developing countries (8, 32), and have recently been associated with large outbreaks in developed countries, including the United States (1, 13).In the presently accepted paradigm for ETEC pathogenesis, fimbrial (or fibrillar) colonization factors (CFs) (10,22,27) mediate colonization of the small intestine, where organisms elaborate heat-labile enterotoxin (LT) and/or heat-stable enterotoxin (ST) (21, 68). Vaccine development has largely focused on the CFs; however, development of a broadly protective ETEC vaccine has been hampered due to the considerable heterogeneity of the known CFs (6,52,58,62).While a number of other surface antigens of ETEC have been described (20,25,51), their utility as potential vaccine candidates has not been effectively explored, largely due to the lack of a suitable animal model for testing. Animal models that have been used in previous studies of ETEC include infant (3, 16-18, 28, 47) and adult (9) mice, rats (34, 35), and rabbits (19,46,51). All of these models have inherent difficulties in utilization or require anesthesia and/or significant surgical manipulation. Some of the models have not been thoroughly evaluated.We sought to develop a murine model of small-intestinal colonization with human ETEC isolates using adult immunocompetent mice. Here, we report our initial use of this model using the prototypical human ETEC H10407 strain. Furthermore, similar to recent studies of porcine ETEC infection of gnotobiotic piglets (2), we demonstrate that elaboration of the heat-labile toxin provides a distinct advantage to the organism in establishing early colonization of the small-intestinal mucosa.

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Section: Discussionmentioning

confidence: 99%

Importance of Heat-Labile Enterotoxin in Colonization of the Adult Mouse Small Intestine by Human EnterotoxigenicEscherichia coliStrains

Allen¹,

Randolph²,

2006

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“…Nevertheless, as stated by Gofhnet et al (1994) Hanset (1991) and Boujenane et al (1991). The subpopulations may often be considered as independent samples (eg, Bradford and Famula, 1984;Duchet-Suchaux et al, 1992;Loisel et al, 1994). The The statistical analysis of the data obtained from these populations was clearly described by Elston and Stewart (1973) and Stewart and Elston (1973).…”

Section: Introductionmentioning

confidence: 99%

Optimal design for the detection of a major gene segregation in crosses between 2 pure lines

Elsen¹,

Roy²

1995

Genet Sel Evol

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Interactions between the Enteric Pathogen and the Host

Mouricout

1997

Advances in Experimental Medicine and Biology

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Genetic control of resistance to enterotoxigenic Escherichia coli in infant mice

Cited by 6 publications

References 12 publications

Importance of Heat-Labile Enterotoxin in Colonization of the Adult Mouse Small Intestine by Human EnterotoxigenicEscherichia coliStrains

Importance of Heat-Labile Enterotoxin in Colonization of the Adult Mouse Small Intestine by Human EnterotoxigenicEscherichia coliStrains

Optimal design for the detection of a major gene segregation in crosses between 2 pure lines

Interactions between the Enteric Pathogen and the Host

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