Genetic control of innate immune responses against cytomegalovirus: MCMV meets its match

Webb, John R.; Lee, S H; Vidal, Silvia M.

doi:10.1038/sj.gene.6363876

Cited by 41 publications

(28 citation statements)

References 150 publications

(138 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the degree of protection against vvHCV.S challenge after HCV-LP immunization are rather disparate between the two strains. This disparity is most likely due to the different genetic backgrounds, for the two strains have been shown to exhibit divergent cytokine responses and susceptibility to various infections (32)(33)(34).…”

Section: Discussionmentioning

confidence: 99%

Immunization with hepatitis C virus-like particles protects mice from recombinant hepatitis C virus-vaccinia infection

Murata

Lechmann

Qiao

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

151

123

View full text Add to dashboard Cite

We have recently demonstrated that immunization with hepatitis C virus-like particles (HCV-LPs) generated in insect cells can elicit both humoral and cellular immune responses in BALB͞c mice. Here, we evaluate the immunogenicity of HCV-LPs in HLA2.1 transgenic (AAD) mice in comparison to DNA immunization. HCV-LP immunization elicited a significantly stronger humoral immune response than DNA immunization. HCV-LP-immunized mice also developed stronger HCV-specific cellular immune responses than DNA-immunized mice as determined by using quantitative enzyme-linked immunospot (ELISpot) assay and intracellular cytokine staining. In BALB͞c mice, immunization with HCV-LPs resulted in a >5 log10 reduction in vaccinia titer when challenged with a recombinant vaccinia expressing the HCV structural proteins (vvHCV.S), as compared to 1 log 10 decrease in DNA immunization. In HLA2.1 transgenic mice, a 1-2 log10 reduction resulted from HCV-LP immunization, whereas no reduction was seen from DNA immunization. Adoptive transfer of lymphocytes from HCV-LP-immunized mice to naive mice provided protection against vvHCV.S challenge, and this transferred immunity can be abrogated by either CD4 or CD8 depletion. Our results suggest that HCV-LPs can induce humoral and cellular immune responses that are protective in a surrogate HCV challenge model and that a strong cellular immunity provided by both CD4 and CD8 effector lymphocytes may be important for protection from HCV infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Immunization with hepatitis C virus-like particles protects mice from recombinant hepatitis C virus-vaccinia infection

Murata

Lechmann

Qiao

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

151

123

View full text Add to dashboard Cite

show abstract

“…Using different strains of mice a susceptibility locus for CMV infection, cmv1, was identified and later shown to encode the Ly49 family of proteins (19). There are at least 14 members and most are expressed on NK and NKT cells, where the majority act as NK inhibitory receptors for MHC I molecules.…”

Section: Innate Immunitymentioning

confidence: 99%

Of Mice and Not Men: Differences between Mouse and Human Immunology

Mestas

Hughes

2004

The Journal of Immunology

2,999

2,322

View full text Add to dashboard Cite

Mice are the experimental tool of choice for the majority of immunologists and the study of their immune responses has yielded tremendous insight into the workings of the human immune system. However, as 65 million years of evolution might suggest, there are significant differences. Here we outline known discrepancies in both innate and adaptive immunity, including: balance of leukocyte subsets, defensins, Toll receptors, inducible NO synthase, the NK inhibitory receptor families Ly49 and KIR, FcR, Ig subsets, the B cell (BLNK, Btk, and λ5) and T cell (ZAP70 and common γ-chain) signaling pathway components, Thy-1, γδ T cells, cytokines and cytokine receptors, Th1/Th2 differentiation, costimulatory molecule expression and function, Ag-presenting function of endothelial cells, and chemokine and chemokine receptor expression. We also provide examples, such as multiple sclerosis and delayed-type hypersensitivity, where complex multicomponent processes differ. Such differences should be taken into account when using mice as preclinical models of human disease.

show abstract

“…In an important paper on the genetic control of innate immune responses against MCMV that appeared in your last issue, Drs JR Webb, S-H Lee and SM Vidal provide an interesting historical perspective of the MCMV infection model from its inception to the discovery of the gene responsible for the phenotype . 1 We have indicated already in 1974, 2 that the susceptibility of different strains of mice to MCMV infection is under genetic control, but our paper has probably escaped the attention of the authors. In the mentioned paper, we have reported that MCMV isolated from wild mice proved to be non-infectious for albino Swiss mice and could not be propagated in cell cultures derived from Swiss embryos.…”

mentioning

confidence: 89%